scholarly journals The purification and characterization of a fatty acid binding protein specific to pig (Sus domesticus) adipose tissue

1990 ◽  
Vol 267 (2) ◽  
pp. 373-378 ◽  
Author(s):  
M K Armstrong ◽  
D A Bernlohr ◽  
J Storch ◽  
S D Clarke
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid ◽  
Blot Analysis ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Polyacrylamide Gel Electrophoresis ◽  
Gel Filtration ◽  
Apparent Dissociation Constant ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

Western-blot analysis using antiserum to 3T3-L1-cell fatty acid binding protein (FABP) revealed that pig adipose tissue contains a 15 kDa protein immunologically similar to the murine protein. This 15 kDa protein was purified from pig adipose tissue by sequential application of Sephadex G-50 gel filtration, cation exchange and covalent chromatography on Thiol-Sepharose-4B. The purity of the pig protein was established by two-dimensional polyacrylamide-gel electrophoresis. Isoelectric focusing indicated that the pig adipose FABP (a-FABP) exists with two charge isoforms (pI 5.1 and 5.2), both of which persist after delipidation. The N-terminus of the purified pig a-FABP was blocked; however, cleavage with CNBr allowed recovery of a 12-amino-acid peptide which was identical with the murine a-FABP sequence (residues 36-48) at 10 of 12 positions. The pig a-FABP bound 12-(9-anthroyloxy)oleic acid saturably and stoichiometrically, with an apparent dissociation constant of 1.0 microM. Northern-blot analysis using the cDNA for the murine 3T3-L1 FABP revealed that the pig a-FABP was expressed exclusively in adipose tissue.

scholarly journals Studies on fatty acid-binding proteins. The diurnal variation shown by rat liver fatty acid-binding protein

1987 ◽  
Vol 242 (3) ◽  
pp. 913-917 ◽  
Author(s):  
T C I Wilkinson ◽  
D C Wilton
Keyword(s):  
Fatty Acid ◽  
Rat Liver ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Polyacrylamide Gel Electrophoresis ◽  
Small Variation ◽  
Fatty Acid Binding Proteins ◽  
Peroxisome Proliferator ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

The concentration of fatty acid-binding protein in rat liver was examined by SDS/polyacrylamide-gel electrophoresis, by Western blotting and by quantifying the fluorescence enhancement achieved on the binding of the fluorescent probe 11-(dansylamino)undecanoic acid. A 2-3-fold increase in the concentration of this protein produced by treatment of rats with the peroxisome proliferator tiadenol was readily detected; however, only a small variation in the concentration of the protein due to a diurnal rhythm was observed. This result contradicts the 7-10-fold variation previously reported for this protein [Hargis, Olson, Clarke & Dempsey (1986) J. Biol. Chem. 261, 1988-1991].

Measuring committed preadipocytes in human adipose tissue from severely obese patients by using adipocyte fatty acid binding protein

2004 ◽  
Vol 287 (5) ◽  
pp. R1132-R1140 ◽  
Author(s):  
Yourka D. Tchoukalova ◽  
Michael G. Sarr ◽  
Michael D. Jensen
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid ◽  
Lipid Droplets ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Human Adipose Tissue ◽  
Cell Number ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Severely Obese

To understand the significance of the reported depot differences in preadipocyte dynamics, we developed a procedure to identify committed preadipocytes in the stromovascular fraction of fresh human adipose tissue. We documented that adipocyte fatty acid binding protein (aP2) is expressed in human preadipocyte clones capable of replication, indicating that can be used as a marker of committed preadipocytes. Because aP2 expression can be induced in macrophages, stromovascular cells were also stained for the macrophage marker CD68. We found aP2+CD68− cells (designated as committed preadipocytes) that did not have lipid droplets (true preadipocytes) and that did have lipid droplets <6.5 μm in diameter (very immature adipocytes). Adipose tissue from subcutaneous, omental, and mesenteric depots was obtained from nine patients undergoing bariatric surgery for measurement of stromovascular cell number, the number of committed preadipocytes (aP2+CD68−), aP2+ macrophages (aP2+CD68+), and aP2− macrophages (aP2−CD68+). The number of committed preadipocytes did not differ significantly between depots but varied >20-fold among individuals. Total cell number, stromovascular cell number, and the number of aP2− macrophages was less ( P < 0.05) in subcutaneous than in omental fat (means ± SE, in millions: subcutaneous, 2.3 ± 0.3, 1.4 ± 0.3, and 0.17 ± 0.08; and omental, 4.8 ± 0.7, 3.8 ± 0.5, and 0.34 ± 0.06); mesenteric depot was intermediate. These data indicate that the cellular composition of adipose tissue varies between depots and between individuals. The ability to quantify committed preadipocytes in fresh adipose tissue should facilitate study of adipose tissue biology.

scholarly journals Association of the Intestinal Fatty Acid-Binding Protein Ala54Thr Polymorphism and Abdominal Adipose Tissue in African-American and Caucasian Women

2005 ◽  
Vol 90 (2) ◽  
pp. 1196-1201 ◽  
Author(s):  
Cristina Lara-Castro ◽  
Gary R. Hunter ◽  
Jennifer C. Lovejoy ◽  
Barbara A. Gower ◽  
José R. Fernández
Keyword(s):  
Body Composition ◽  
Adipose Tissue ◽  
African American ◽  
Fatty Acid ◽  
Ethnic Group ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Fatty Acid Binding ◽  
Abdominal Adipose Tissue ◽  
Acid Binding Protein

Genetic variants in the intestinal fatty acid-binding protein-2 (FABP2) gene have been associated with body composition phenotypes. We examined the association between the Ala54Thr variant in the FABP2 gene and levels of visceral (VAT) and sc (SAAT) abdominal fat in a group of 223 premenopausal African-American (n = 103) and Caucasian (n = 120) women. Subjects were genotyped for the marker. In addition, body composition was assessed by dual energy x-ray absorptiometry, and VAT was determined from a single computed tomography scan. The frequency of the Thr mutant allele did not differ significantly by ethnic group. After adjusting for total body fat, total abdominal adipose tissue (TAT) and SAAT were significantly lower in carriers of either one or two copies of the mutant Thr allele (P &lt; 0.01). There was no association between total fat mass or VAT and the FABP2 polymorphism. Separate analyses by ethnic group showed that the association between the polymorphism and TAT and SAAT was observed in Caucasian (P &lt; 0.01), but not in African-American (not significant), women. We conclude that women carriers of the FABP2 Thr allele have lower TAT and SAAT than noncarriers of the mutation. This association is present in Caucasian, but not in African-American, women.

Presence of fatty-acid-binding protein 4 expression in human epicardial adipose tissue in metabolic syndrome

2008 ◽  
Vol 17 (6) ◽  
pp. 392-398 ◽  
Author(s):  
Burcak Vural ◽  
Fatmahan Atalar ◽  
Cavlan Ciftci ◽  
Ayse Demirkan ◽  
Belgin Susleyici-Duman ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Adipose Tissue ◽  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Epicardial Adipose Tissue ◽  
Binding Protein ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

scholarly journals Fatty acid binding protein expression in different adipose tissue depots from lean and obese individuals

Diabetologia ◽  
2001 ◽  
Vol 44 (10) ◽  
pp. 1268-1273 ◽  
Author(s):  
R. M. Fisher ◽  
P. Eriksson ◽  
J. Hoffstedt ◽  
G. S. Hotamisligil ◽  
A. Thörne ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid ◽  
Protein Expression ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Adipose Tissue Depots
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