A tissue-specific nuclear factor binds to multiple sites in the human insulin-gene enhancer

1990 ◽  
Vol 265 (3) ◽  
pp. 932-932
Keyword(s):  
Human Insulin ◽  
Insulin Gene ◽  
Nuclear Factor ◽  
Tissue Specific ◽  
Human Insulin Gene ◽  
Gene Enhancer

scholarly journals A tissue-specific nuclear factor binds to multiple sites in the human insulin-gene enhancer

1989 ◽  
Vol 264 (1) ◽  
pp. 233-239 ◽  
Author(s):  
D S W Boam ◽  
K Docherty
Keyword(s):  
Cell Line ◽  
Human Insulin ◽  
Specific Binding ◽  
Binding Activity ◽  
Insulin Gene ◽  
Nuclear Factor ◽  
Sequence Element ◽  
Upstream Site ◽  
Human Insulin Gene ◽  
Specific Regulation

Sequence-specific binding of proteins from an insulin-secreting cell line (RINm-5F) to the human insulin-gene 5′ region were examined by gel-retardation and methylation-interference analysis. Specific binding of a nuclear factor to sites between nucleotides -210 to -217 and -77 to -84 was detected. The same binding activity was shown at an upstream site (-313 to -320) with low affinity. Studies using mutated binding-site probes delineated a sequence 5′-C(T/C)CTAATG-3′ for high-affinity interactions. This binding activity was also present in another insulin-producing cell line (HIT.T15), but not in extracts from cell lines that did not express the insulin gene (HeLa, HL60). Cross-species comparisons show that this sequence element is highly conserved and may thus play an important role in the cell-specific regulation of insulin-gene transcription.

HEPATOCYTE NUCLEAR FACTOR-1α INHIBITS INSULIN PROMOTER FACTOR 1-DEPENDENT TRANSACTIVATION OF THE HUMAN INSULIN GENE

2001 ◽  
Vol 27 (1-2) ◽  
pp. 63-74 ◽  
Author(s):  
Kenichi Yamakawa ◽  
Hironori Yamasaki ◽  
Masako Ozaki ◽  
Mikako-Degawa Yamauchi ◽  
Naruhiro Fujita ◽  
...  
Keyword(s):  
Human Insulin ◽  
Insulin Gene ◽  
Nuclear Factor ◽  
Hepatocyte Nuclear Factor ◽  
Human Insulin Gene ◽  
Insulin Promoter ◽  
Hepatocyte Nuclear Factor 1Α

scholarly journals The helix-loop-helix transcription factor USF (upstream stimulating factor) binds to a regulatory sequence of the human insulin gene enhancer

1993 ◽  
Vol 295 (1) ◽  
pp. 233-237 ◽  
Author(s):  
M L Read ◽  
A R Clark ◽  
K Docherty
Keyword(s):  
Transcription Factor ◽  
Human Insulin ◽  
Insulin Gene ◽  
Regulatory Sequence ◽  
Mobility Shift ◽  
Helix Loop Helix ◽  
Cell Extracts ◽  
Human Insulin Gene ◽  
Gene Enhancer ◽  
Stimulating Factor

Two important sequence elements, designated insulin enhancer binding site 1 (IEB1) or NIR and IEB2 or FAR, are involved in regulating expression of the rat insulin I gene. These elements bind a helix-loop-helix transcription factor, insulin enhancer factor 1 (IEF1). The IEB1 site is highly conserved among insulin genes but the IEB2 site is not conserved. To investigate the factors binding at the equivalent IEB1 and IEB2 sites in the human insulin gene enhancer, electrophoretic mobility shift assays were performed using a variety of cell extracts and probes specific for the homologous IEB1 and IEB2 sites. The results indicate that a factor with similar tissue distribution and binding characteristics to those of IEF1 binds to the IEB1 site in the human insulin gene, but that a separate factor, identified as the adenovirus major late transcription factor [MLTF, or upstream stimulating factor (USF)] binds to the IEB2 site.

scholarly journals Elevated Glucose Attenuates Human Insulin Gene Promoter Activity in INS-1 Pancreatic β-Cells via Reduced Nuclear Factor Binding to the A5/Core and Z Element

2005 ◽  
Vol 19 (5) ◽  
pp. 1343-1360 ◽  
Author(s):  
Maria F. Pino ◽  
Diana Z. Ye ◽  
Katrina D. Linning ◽  
Christopher D. Green ◽  
Barton Wicksteed ◽  
...  
Keyword(s):  
Human Insulin ◽  
Promoter Activity ◽  
Gene Promoter ◽  
Insulin Gene ◽  
Nuclear Factor ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
Factor Binding ◽  
Human Insulin Gene ◽  
Elevated Glucose

Human Insulin Gene Is a Target Gene of Hepatocyte Nuclear Factor-1α (HNF-1α) and HNF-1β

1999 ◽  
Vol 263 (2) ◽  
pp. 566-569 ◽  
Author(s):  
Kohei Okita ◽  
Qin Yang ◽  
Kazuya Yamagata ◽  
Kerstin A. Hangenfeldt ◽  
Jun-ichiro Miyagawa ◽  
...  
Keyword(s):  
Human Insulin ◽  
Target Gene ◽  
Insulin Gene ◽  
Nuclear Factor ◽  
Hepatocyte Nuclear Factor ◽  
Human Insulin Gene ◽  
Hepatocyte Nuclear Factor 1Α

scholarly journals Human insulin gene enhancer-binding proteins in pancreatic α and β cell lines

FEBS Letters ◽  
1993 ◽  
Vol 329 (1-2) ◽  
pp. 139-143 ◽  
Author(s):  
Andrew R. Clark ◽  
Helle V. Petersen ◽  
Martin L. Read ◽  
Valerie Scott ◽  
Birgitte K. Michelsen ◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Insulin ◽  
Binding Proteins ◽  
Insulin Gene ◽  
Β Cell ◽  
Enhancer Binding Proteins ◽  
Human Insulin Gene ◽  
Gene Enhancer

scholarly journals An insulinoma nuclear factor binding to GGGCCC motifs in human insulin gene

1993 ◽  
Vol 21 (7) ◽  
pp. 1595-1600 ◽  
Author(s):  
Louise Reibel ◽  
Corinne Besnard ◽  
Patrick Lores ◽  
Jacques Jami ◽  
Gerard Gacon
Keyword(s):  
Human Insulin ◽  
Insulin Gene ◽  
Nuclear Factor ◽  
Factor Binding ◽  
Human Insulin Gene

scholarly journals Tissue-Specific Methylation of Human Insulin Gene and PCR Assay for Monitoring Beta Cell Death

PLoS ONE ◽  
2014 ◽  
Vol 9 (4) ◽  
pp. e94591 ◽  
Author(s):  
Mohamed I. Husseiny ◽  
Alexander Kaye ◽  
Emily Zebadua ◽  
Fouad Kandeel ◽  
Kevin Ferreri
Keyword(s):  
Cell Death ◽  
Beta Cell ◽  
Human Insulin ◽  
Insulin Gene ◽  
Pcr Assay ◽  
Tissue Specific ◽  
Beta Cell Death ◽  
Human Insulin Gene

scholarly journals Identification of a Novel PDX-1 Binding Site in the Human Insulin Gene Enhancer

2004 ◽  
Vol 279 (21) ◽  
pp. 22228-22235 ◽  
Author(s):  
John Le Lay ◽  
Taka-aki Matsuoka ◽  
Eva Henderson ◽  
Roland Stein
Keyword(s):  
Binding Site ◽  
Human Insulin ◽  
Insulin Gene ◽  
Human Insulin Gene ◽  
Gene Enhancer

Lack of association of the polymorphic locus in the 5'-flanking region of the human insulin gene and diabetes in American blacks

Diabetes ◽  
1985 ◽  
Vol 34 (5) ◽  
pp. 433-439 ◽  
Author(s):  
S. Elbein ◽  
P. Rotwein ◽  
M. A. Permutt ◽  
G. I. Bell ◽  
N. Sanz ◽  
...  
Keyword(s):  
Human Insulin ◽  
Polymorphic Locus ◽  
Insulin Gene ◽  
Human Insulin Gene ◽  
Flanking Region ◽  
American Blacks
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