Phospholipase D activation is functionally linked to superoxide generation in the human neutrophil

1990 ◽  
Vol 265 (3) ◽  
pp. 932-932 ◽  
Keyword(s):  
Phospholipase D ◽  
Human Neutrophil ◽  
Superoxide Generation

scholarly journals Phospholipase D activation is functionally linked to superoxide generation in the human neutrophil

1989 ◽  
Vol 264 (2) ◽  
pp. 617-620 ◽  
Author(s):  
R W Bonser ◽  
N T Thompson ◽  
R W Randall ◽  
L G Garland
Keyword(s):  
Phosphatidic Acid ◽  
Phospholipase D ◽  
Human Neutrophil ◽  
Superoxide Production ◽  
Superoxide Generation ◽  
Inositol 1 ◽  
Opsonized Zymosan

Neutrophils stimulated with formylmethionyl-leucylphenylalanine (fMet-Leu-Phe) in the presence of butanol and ethanol formed phosphatidyl alcohols through a phospholipase D mechanism. The alcohols inhibited phosphatidic acid and diradylglycerol (DRG) formation, but did not block inositol 1, 4, 5-trisphosphate release. fMet-Leu-Phe-stimulated superoxide production was inhibited by alcohol concentrations which blocked DRG formation, whereas opsonized-zymosan-stimulated superoxide production was only partially decreased. These results suggest that phospholipase D activation is functionally linked to superoxide production in the human neutrophil.

scholarly journals Novel polyisoprenyl phosphates block phospholipase D and human neutrophil activation in vitro and murine peritoneal inflammation in vivo

2005 ◽  
Vol 146 (3) ◽  
pp. 344-351 ◽  
Author(s):  
Bruce D Levy ◽  
Lorraine Hickey ◽  
Andrew J Morris ◽  
Mykol Larvie ◽  
Raquel Keledjian ◽  
...  
Keyword(s):  
Phospholipase D ◽  
Human Neutrophil ◽  
Neutrophil Activation ◽  
Peritoneal Inflammation

Activation of human neutrophil phospholipase D by three separable mechanisms

1990 ◽  
Vol 4 (2) ◽  
pp. 208-214 ◽  
Author(s):  
Sandra L. Reinhold ◽  
Stephen M. Prescott ◽  
Guy A. Zimmerman ◽  
Thomas M. McIntyre
Keyword(s):  
Phospholipase D ◽  
Human Neutrophil

scholarly journals Trp-Lys-Tyr-Met-Val-D-Met stimulates superoxide generation and killing of Staphylococcus aureus via phospholipase D activation in human monocytes

1999 ◽  
Vol 65 (2) ◽  
pp. 241-248 ◽  
Author(s):  
Yoe-Sik Bae ◽  
Seong-A Ju ◽  
Ji Yung Kim ◽  
Jeong Kon Seo ◽  
Suk Hwan Baek ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Phospholipase D ◽  
Human Monocytes ◽  
Superoxide Generation

scholarly journals The temporal relationship between phospholipase activation, diradylglycerol formation and superoxide production in the human neutrophil

1990 ◽  
Vol 271 (1) ◽  
pp. 209-213 ◽  
Author(s):  
N T Thompson ◽  
J E Tateson ◽  
R W Randall ◽  
G D Spacey ◽  
R W Bonser ◽  
...  
Keyword(s):  
Phospholipase D ◽  
Human Neutrophil ◽  
Second Messengers ◽  
Superoxide Production ◽  
Human Neutrophils ◽  
Inositol 1,4,5 Trisphosphate ◽  
Phosphatidate Phosphohydrolase ◽  
Time Courses ◽  
Rapid Activation ◽  
Stimulation Of

Fluctuations in the amounts of choline, inositol 1,4,5-trisphosphate (IP3) and diradylglycerol have been used to monitor phospholipase activation in the human neutrophil. Stimulation of human neutrophils by formylmethionyl-leucylphenylalanine (fMet-Leu-Phe) resulted in a rapid activation of both phosphatidylinositol 4,5-bisphosphate breakdown by phospholipase C and phosphatidylcholine breakdown by phospholipase D. Diradylglycerol accumulation occurred more slowly than that of either choline or IP3 and was inhibited by 30 mM-butanol, suggesting that the bulk was derived from the phospholipase D pathway via phosphatidate phosphohydrolase. Consistent with this is the observation that choline and diradylglycerol are produced in similar amounts. 1,2-Diacylglycerol (DAG) and 1-O-alkyl-2-acyl-sn-glycerol species accumulated with different time courses, indicating that one or more steps in the phospholipase D pathway was selective for the diacyl species. Superoxide production by fMet-Leu-Phe-stimulated neutrophils paralleled DAG accumulation over the first 5 min, but thereafter this production stopped, despite the fact that DAG remained elevated. We conclude that DAG derived from the phospholipase D pathway is only one of the second messengers important in controlling this functional response.

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