scholarly journals The Escherichia coli cysG gene encodes S-adenosylmethionine-dependent uroporphyrinogen III methylase

1990 ◽  
Vol 265 (3) ◽  
pp. 725-729 ◽  
Author(s):  
M J Warren ◽  
C A Roessner ◽  
P J Santander ◽  
A I Scott
Keyword(s):  
Escherichia Coli ◽  
Methyl Ester ◽  
Absorption Spectra ◽  
Uroporphyrinogen Iii Methylase

The Escherichia coli cysG gene was successfully subcloned and over-expressed to produce a 52 kDa protein that was purified to homogeneity. This protein was shown to catalyse the S-adenosylmethionine-dependent methylation of uroporphyrinogen III to give a product identified as sirohydrochlorin on the basis of its absorption spectra, incorporation of 14C label from S-adenosyl[Me-14C]methionine and mass and 1H-n.m.r. spectra of its octamethyl ester. Further confirmation of the structure was obtained from a 14C-n.m.r. spectrum of the methyl ester produced by incubation of the methylase with uroporphyrinogen III, derived from [4.6-13C2]porphobilinogen, and S-adenosyl[Me-13C]methionine.

7-β-d-galactopyranosyloxycoumarin-4-acetic acid and its methyl ester as substrates for the β-d-galactosidase of Escherichia coli

1990 ◽  
Vol 197 ◽  
pp. 295-301 ◽  
Author(s):  
Neil Baggett ◽  
Martin A. Case ◽  
Paul R. Darby ◽  
Charles J. Gray
Keyword(s):  
Escherichia Coli ◽  
Acetic Acid ◽  
Methyl Ester

scholarly journals Conformation of Escherichia coli Glutamic Acid tRNA II as Studied by Hydrogen-Tritium Exchange Catalyzed by Cysteine Methyl Ester

1976 ◽  
Vol 64 (1) ◽  
pp. 27-34 ◽  
Author(s):  
Yusuke WATAYA ◽  
Shigeru LIDA ◽  
Ichiro KUDO ◽  
Hikoya HAYATSU ◽  
Ziro OHASHI ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Methyl Ester ◽  
Glutamic Acid

scholarly journals Glutamyl-γ-methyl ester acts as a methionine analogue inEscherichia coli: analogue resistant mutants map at themetJandmetKloci

1979 ◽  
Vol 33 (1) ◽  
pp. 49-55 ◽  
Author(s):  
Jan Kraus ◽  
Dieter Soll ◽  
K. Brooks Low
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Methyl Ester ◽  
Gene Order ◽  
Cross Resistance ◽  
Inescherichia Coli ◽  
Amino Acid Analogues ◽  
Resistant Mutants ◽  
K 12

SUMMARYEscherichia coliK-12 mutants resistant to glutamyl-γ-methyl ester were isolated. A mutation leading to resistance of up to 1·4 mg/ml of the methionine analogue maps at min 63 and is 13% cotransducible withserAindicating an alteration in themetKgene. Another mutation leading to resistance to 3 mg/ml of the analogue and cross-resistance to other amino acid analogues maps at min 87. This mutation, which has the phenotype of MetJ−, is shown to be situated between theglpKandmetBgenes and thus indicates a different gene order from the published one.

scholarly journals DEVELOPMENT OF DNA BIOSENSOR BASED ON SILVER NANOPARTICLES UV-Vis ABSORPTION SPECTRA FOR Escherichia coli DETECTION

2015 ◽  
Vol 2 (1) ◽  
pp. 382 ◽  
Author(s):  
Ruth Chrisnasari ◽  
Antonius Loren Wijaya ◽  
Maria Goretti Marianti Purwanto
Keyword(s):  
Escherichia Coli ◽  
Silver Nanoparticles ◽  
Absorption Spectra ◽  
Magnetic Beads ◽  
Dna Isolation ◽  
Dna Biosensor ◽  
Isolation Method ◽  
Isolation Methods ◽  
Spectra Properties ◽  
Magnetic Field Condition

<p>In this research we reported the synthesis of oligonucleotide-silver nanoparticle (OSN) conjugates and demonstrated their use along with magnetic beads as biosensor for Escherichia coli detection under magnetic field condition. Oligonucleotide DNA probes were conjugated on silver nanoparticles using alkanethiols linker. Two kinds of alkanethiols linker, 11-mercaptoundodecanoic acid (11-MUDA) and 16-mercaptophexadecanoic acid (16-MHDA) were compared to get the best probe conjugation yield and OSN UV-Vis absorption spectra properties. Three different methods of Escherichia coli DNA isolation i.e. Chen and Kuo (1993), Phenol Chloroform Isoamylalcohol (PCI) extraction and boiling lysis were also compared to explore the performance of the biosensor towards the DNA target purity. Detection process through hybridization between the DNA probe and the target was carried out at 55oC for 1 hour incubation time. The results showed that 16-MHDA gave higher conjugation yield and higher OSN UV-Vis absorption spectra than 11-MUDA. The biosensor was able to detect the presence of the DNA target which was isolated from the three isolation methods. The best detection signal was achieved by Chen and Kuo isolation method in which it could detect the presence of the DNA target up to 1.3 ng/µL.</p><p><br /><strong>Keywords</strong>: DNA biosensor, Silver Nanoparticles, Escherichia coli</p>

Synthesis of theanine from glutamic acid γ-methyl ester and ethylamine catalyzed by Escherichia coli having γ-glutamyltranspeptidase activity

2010 ◽  
Vol 32 (8) ◽  
pp. 1147-1150 ◽  
Author(s):  
Fei Zhang ◽  
Qing-Zhong Zheng ◽  
Qing-Cai Jiao ◽  
Jun-Zhong Liu ◽  
Gen-Hai Zhao
Keyword(s):  
Escherichia Coli ◽  
Methyl Ester ◽  
Glutamic Acid
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