scholarly journals A kinetic investigation of the acyl-CoA oxidase reaction with the use of a novel spectrophotometric assay. Inhibition by acetyl-CoA, CoA and FMN

1989 ◽  
Vol 263 (1) ◽  
pp. 297-299 ◽  
Author(s):  
R Hovik ◽  
H Osmundsen
Keyword(s):  
Oxidase Activity ◽  
Spectrophotometric Assay ◽  
Strong Absorption ◽  
Kinetic Investigation ◽  
Kinetic Characteristics ◽  
Direct Reading ◽  
Acetyl Coa ◽  
Acyl Coa Oxidase ◽  
Oxidase Reaction

A direct-reading spectrophotometric assay for acyl-CoA oxidase activity is described. The assay is based on the strong absorption at 300 nm of deca-2-trans,4-cis-dienoyl-CoA, the product of oxidation of dec-4-cis-enoyl-CoA. By use of this assay, acetyl-CoA, CoA and FMN were found to be inhibitors of acyl-CoA oxidase, but with distinctly different kinetic characteristics.

scholarly journals Factors which affect the activity of purified rat liver acyl-CoA oxidase

1993 ◽  
Vol 290 (1) ◽  
pp. 97-102 ◽  
Author(s):  
R Hovik ◽  
H Osmundsen
Keyword(s):  
Enzyme Activity ◽  
Rat Liver ◽  
Oxidase Activity ◽  
Substrate Inhibition ◽  
Critical Micellar Concentration ◽  
Competitive Inhibitor ◽  
Acetyl Coa ◽  
Acyl Coa Oxidase ◽  
Triton X

The activity of the enzyme acyl-CoA oxidase (EC 1.3.99.3) is influenced by detergents. At concentrations above the critical micellar concentration, Triton X-100, Triton X-114 and Thesit stimulate oxidase activity. Lower concentrations of Triton X-100 and Triton X-114 render the acyl-CoA oxidase less sensitive towards substrate inhibition by palmitoyl-CoA or dec-4-cis-enoyl-CoA. Other detergents inhibited the enzyme activity. CoA was found to be a relatively powerful competitive inhibitor of the enzyme, with a Ki, slope value of 63 +/- 3 microM. This inhibition is dependent on an intact CoA molecule, as dephospho-CoA, dethio-CoA and acetyl-CoA are less potent inhibitors of the enzyme. Dec-2-trans-enoyl-CoA is a product-inhibitor of acyl-CoA oxidase, with a Ki, slope value of 7 +/- 1 microM.

scholarly journals A sensitive spectrophotometric assay for peroxisomal acyl-CoA oxidase

1985 ◽  
Vol 227 (1) ◽  
pp. 205-210 ◽  
Author(s):  
G M Small ◽  
K Burdett ◽  
M J Connock
Keyword(s):  
Oxidase Activity ◽  
Mouse Liver ◽  
Enzyme Concentration ◽  
Fatty Acyl ◽  
Spectrophotometric Assay ◽  
Acid Oxidase ◽  
Rat Intestine ◽  
Amino Acid Oxidase ◽  
Acyl Coa Oxidase ◽  
First Time

A simple spectrophotometric assay was developed for peroxisomal fatty acyl-CoA oxidase activity. The assay, based on the H2O2-dependent oxidation of leuco-dichlorofluorescein catalysed by exogenous peroxidase, is more sensitive than methods previously described. By using mouse liver samples, cofactor requirements were assessed and a linear relationship was demonstrated between dye oxidation and enzyme concentration. By using this assay on subcellular fractions, palmitoyl-CoA oxidase activity was localized for the first time in microperoxisomes of rat intestine. The assay was also adapted to measure D-amino acid oxidase activity, demonstrating the versatility of this method for measuring activity of other H2O2-producing oxidases.

A spectrophotometric assay for monoamine oxidase activity with 2, 4-dinitrophenylhydrazine as a derivatized reagent

2016 ◽  
Vol 512 ◽  
pp. 18-25 ◽  
Author(s):  
Guili Huang ◽  
Fei Zhu ◽  
Yuhang Chen ◽  
Shiqiang Chen ◽  
Zhonghong Liu ◽  
...  
Keyword(s):  
Monoamine Oxidase ◽  
Oxidase Activity ◽  
Spectrophotometric Assay ◽  
Monoamine Oxidase Activity

Hepatic peroxisome proliferation in vitamin A-deficient mice without a simultaneous increase in peroxisomal Acyl-CoA oxidase activity

1996 ◽  
Vol 51 (6) ◽  
pp. 821-827 ◽  
Author(s):  
Anna-Karin Sohlenius ◽  
Maria Reinfeldt ◽  
Katrin Bäckström ◽  
Anders Bergstrand ◽  
Joseph W. DePierre
Keyword(s):  
Vitamin A ◽  
Oxidase Activity ◽  
Simultaneous Increase ◽  
Peroxisome Proliferation ◽  
Acyl Coa Oxidase ◽  
Deficient Mice

A rapid spectrophotometric assay of phenylpyruvate oxidase activity

1972 ◽  
Vol 50 (1) ◽  
pp. 180-186 ◽  
Author(s):  
Brian L. Goodwin ◽  
Elizabeth G. Werner
Keyword(s):  
Oxidase Activity ◽  
Spectrophotometric Assay
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