scholarly journals Developmental regulation of the mRNAs for elastins a, b and c in foetal-calf nuchal ligament and aorta

1989 ◽  
Vol 261 (1) ◽  
pp. 227-232 ◽  
Author(s):  
R P Paulovic ◽  
R A Anwar
Keyword(s):  
Developmental Regulation ◽  
Developmentally Regulated ◽  
Foetal Development ◽  
Tissue Specific ◽  
Nuchal Ligament ◽  
Self Aggregation

The data presented clearly suggest that relative amounts of mRNAs for elastins a, b and c are developmentally regulated in foetal-calf nuchal ligament and aorta and that this regulation is tissue-specific. In nuchal ligament, at earlier stages of foetal development, the relative amounts of mRNAs for elastins a and b are very low. After the foetal age of about 6 months the relative amount of mRNA for elastin b begins to increase. This is followed by an increase in the relative amount of mRNA for elastin a. In aorta, with increasing foetal age, the relative amounts of mRNAs for elastins b and c increase and decrease alternately. The relative amounts of mRNA for elastin a remain low, with only marginal increases with foetal age. A possible self-aggregation role of elastin a in elastogenesis is proposed.

scholarly journals Identification and developmental regulation of a neuron-specific subunit of cytoplasmic dynein.

1996 ◽  
Vol 7 (2) ◽  
pp. 331-343 ◽  
Author(s):  
K K Pfister ◽  
M W Salata ◽  
J F Dillman ◽  
E Torre ◽  
R J Lye
Keyword(s):  
Axonal Transport ◽  
Developmental Regulation ◽  
Cytoplasmic Dynein ◽  
Retrograde Axonal Transport ◽  
Protein Isoforms ◽  
Cultured Neurons ◽  
Developmentally Regulated ◽  
Time Period ◽  
Intermediate Chains

Cytoplasmic dynein is the microtubule minus-end-directed motor for the retrograde axonal transport of membranous organelles. Because of its similarity to the intermediate chains of flagellar dynein, the 74-kDa intermediate chain (IC74) subunit of dynein is thought to be involved in binding dynein to its membranous organelle cargo. Previously, we identified six isoforms of the IC74 cytoplasmic dynein subunit in the brain. We further demonstrated that cultured glia and neurons expressed different dynein IC74 isoforms and phospho-isoforms. Two isoforms were observed when dynein from glia was analyzed. When dynein from cultured neurons was analyzed, six IC74 isoforms were observed, although the relative amounts of the dynein isoforms from cultured neurons differed from those found in dynein from brain. To better understand the role of the neuronal IC74 isoforms and identify neuron-specific IC74 dynein subunits, the expression of the IC74 protein isoforms and mRNAs of various tissues were compared. As a result of this comparison, the identity of each of the isoform spots observed on two-dimensional gels was correlated with the products of each of the IC74 mRNAs. We also found that between the fifteenth day of gestation (E15) and the fifth day after birth (P5), the relative expression of the IC74 protein isoforms changes, demonstrating that the expression of IC74 isoforms is developmentally regulated in brain. During this time period, there is relatively little change in the abundance of the various IC74 mRNAs. The E15 to P5 time period is one of rapid process extension and initial pattern formation in the rat brain. This result indicates that the changes in neuronal IC74 isoforms coincide with neuronal differentiation, in particular the extension of processes. This suggests a role for the neuronal IC74 isoforms in the establishment or regulation of retrograde axonal transport.

scholarly journals Alternative Splicing of the Drosophila Dscam Pre-mRNA Is Both Temporally and Spatially Regulated

Genetics ◽  
2001 ◽  
Vol 159 (2) ◽  
pp. 599-608
Author(s):  
Alicia M Celotto ◽  
Brenton R Graveley
Keyword(s):  
Alternative Splicing ◽  
Axon Guidance ◽  
Neural Development ◽  
Cell Adhesion Molecule ◽  
Developmental Regulation ◽  
Mrna Isoforms ◽  
Developmentally Regulated ◽  
Early Embryos ◽  
Exon 4

Abstract The Drosophila melanogaster Down syndrome cell adhesion molecule (Dscam) gene encodes an axon guidance receptor that can express 38,016 different mRNAs by virtue of alternative splicing. The Dscam gene contains 95 alternative exons that are organized into four clusters of 12, 48, 33, and 2 exons each. Although numerous Dscam mRNA isoforms can be synthesized, it remains to be determined whether different Dscam isoforms are synthesized at different times in development or in different tissues. We have investigated the alternative splicing of the Dscam exon 4 cluster, which contains 12 mutually exclusive alternative exons, and found that Dscam exon 4 alternative splicing is developmentally regulated. The most highly regulated exon, 4.2, is infrequently used in early embryos but is the predominant exon 4 variant used in adults. Moreover, the developmental regulation of exon 4.2 alternative splicing is conserved in D. yakuba. In addition, different adult tissues express distinct collections of Dscam mRNA isoforms. Given the role of Dscam in neural development, these results suggest that the regulation of alternative splicing plays an important role in determining the specificity of neuronal wiring. In addition, this work provides a framework to determine the mechanisms by which complex alternative splicing events are regulated.

Role of Akt signaling pathway regulation in the speckled mousebird (Colius striatus) during torpor displays tissue specific responses

2020 ◽  
Vol 75 ◽  
pp. 109763
Author(s):  
Stuart R. Green ◽  
Rasha Al-Attar ◽  
Andrew E. McKechnie ◽  
Samantha Naidoo ◽  
Kenneth B. Storey
Keyword(s):  
Signaling Pathway ◽  
Akt Signaling ◽  
Akt Signaling Pathway ◽  
Tissue Specific ◽  
Pathway Regulation

Developmental regulation of the PsbS gene expression in spinach seedlings: the role of phytochrome

1996 ◽  
Vol 31 (4) ◽  
pp. 793-802 ◽  
Author(s):  
Iwona Adamska ◽  
Christiane Funk ◽  
Gernot Renger ◽  
Bertil Andersson
Keyword(s):  
Gene Expression ◽  
Developmental Regulation ◽  
Psbs Gene

Tissue-specific and developmental regulation of rod opsin chimeric genes in transgenic mice

Neuron ◽  
1991 ◽  
Vol 6 (2) ◽  
pp. 201-210 ◽  
Author(s):  
Janis Lem ◽  
Meredithe L. Applebury ◽  
Jeffrey D. Falk ◽  
John G. Flannery ◽  
Melvin I. Simon
Keyword(s):  
Transgenic Mice ◽  
Developmental Regulation ◽  
Tissue Specific ◽  
Chimeric Genes
Sign in / Sign up

Export Citation Format

Share Document