scholarly journals Retrieval of precursors for white-type adipose conversion in brown adipose tissue

1988 ◽  
Vol 255 (3) ◽  
pp. 849-854 ◽  
Author(s):  
C M Poissonnet ◽  
M Ouagued ◽  
Y Aron ◽  
J Y Pello ◽  
E Swierczewski ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Gradient Centrifugation ◽  
Cellular Compartment ◽  
Percoll Density Gradient Centrifugation ◽  
Brown Adipose ◽  
Adipose Conversion ◽  
Percoll Density Gradient ◽  
The One

A cellular compartment from brown adipose tissue (BAT) of newborn rats was isolated by Percoll-density-gradient centrifugation and was shown to proliferate and to undergo adipose conversion in vitro in primary culture. The features of the effector requirement for adipose conversion as well as the differentiated morphological and biochemical phenotype are almost identical with that of a compartment designated HCF, from white adipose tissue (WAT). A possible role for these precursors from BAT and WAT in the involution of BAT into WAT, on the one hand, and in the development of brown adipose cells among typical WAT deposits, on the other, is discussed.

scholarly journals TAF7L modulates brown adipose tissue formation

eLife ◽  
2014 ◽  
Vol 3 ◽  
Author(s):  
Haiying Zhou ◽  
Bo Wan ◽  
Ivan Grubisic ◽  
Tommy Kaplan ◽  
Robert Tjian
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Core Promoter ◽  
Uncoupling Protein ◽  
Dna Looping ◽  
Chromatin Interactions ◽  
Brown Adipose ◽  
Important Regulator

Brown adipose tissue (BAT) plays an essential role in metabolic homeostasis by dissipating energy via thermogenesis through uncoupling protein 1 (UCP1). Previously, we reported that the TATA-binding protein associated factor 7L (TAF7L) is an important regulator of white adipose tissue (WAT) differentiation. In this study, we show that TAF7L also serves as a molecular switch between brown fat and muscle lineages in vivo and in vitro. In adipose tissue, TAF7L-containing TFIID complexes associate with PPARγ to mediate DNA looping between distal enhancers and core promoter elements. Our findings suggest that the presence of the tissue-specific TAF7L subunit in TFIID functions to promote long-range chromatin interactions during BAT lineage specification.

Effect of sucrose-induced overfeeding on brown adipose tissue—With special reference to in vitro thermogenesis and fatty acids compositions

1995 ◽  
Vol 20 (6) ◽  
pp. 477-484 ◽  
Author(s):  
Akihiro Kuroshima ◽  
Tomie Ohno ◽  
Mitsuru Moriya ◽  
Hiroshi Ohinata ◽  
Takehiro Yahata ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Special Reference ◽  
Brown Adipose

scholarly journals Dopamine receptor D1- and D2-agonists do not spark brown adipose tissue thermogenesis in mice

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Francesca-Maria Raffaelli ◽  
Julia Resch ◽  
Rebecca Oelkrug ◽  
K. Alexander Iwen ◽  
Jens Mittag
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Dopamine Receptor ◽  
Ex Vivo ◽  
Potential Target ◽  
D2 Agonist ◽  
Obesity And Diabetes ◽  
Brown Adipose

AbstractBrown adipose tissue (BAT) thermogenesis is considered a potential target for treatment of obesity and diabetes. In vitro data suggest dopamine receptor signaling as a promising approach; however, the biological relevance of dopamine receptors in the direct activation of BAT thermogenesis in vivo remains unclear. We investigated BAT thermogenesis in vivo in mice using peripheral administration of D1-agonist SKF38393 or D2-agonist Sumanirole, infrared thermography, and in-depth molecular analyses of potential target tissues; and ex vivo in BAT explants to identify direct effects on key thermogenic markers. Acute in vivo treatment with the D1- or D2-agonist caused a short spike or brief decrease in BAT temperature, respectively. However, repeated daily administration did not induce lasting effects on BAT thermogenesis. Likewise, neither agonist directly affected Ucp1 or Dio2 mRNA expression in BAT explants. Taken together, the investigated agonists do not seem to exert lasting and physiologically relevant effects on BAT thermogenesis after peripheral administration, demonstrating that D1- and D2-receptors in iBAT are unlikely to constitute targets for obesity treatment via BAT activation.

Rapid changes in metabolic cold defense and GDP binding to brown adipose tissue mitochondria of rat pups

1993 ◽  
Vol 264 (5) ◽  
pp. R1017-R1023 ◽  
Author(s):  
G. Kortner ◽  
K. Schildhauer ◽  
O. Petrova ◽  
I. Schmidt
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Time Course ◽  
Developmental Changes ◽  
Whole Body ◽  
Rat Pups ◽  
Brown Adipose ◽  
Rapid Changes ◽  
Whole Body Metabolism

To determine developmental changes of brown adipose tissue (BAT) thermogenic activity at defined circadian and thermal states, we evaluated the time course of cold-induced increases of in vitro guanosine 5'-diphosphate (GDP) binding in parallel with whole body metabolism (oxygen consumption, VO2) and core temperature (Tc) in 1- to 11-day-old rat pups. During the maximum phase of the juvenile diurnal cycle, Tc of littermates was recorded continuously and VO2 alternately until 2 min before animals were killed for removal of interscapular BAT. GDP binding after 1.5 h at thermoneutrality and its increase during physiologically comparable cold loads were significantly lower in 1-day-old pups than in 5- and 11-day-old pups. Cold defense was activated more rapidly in the older pups, but GDP binding in even the 1-day-old pups was significantly increased during the second 10-min period of cold exposure. We conclude that rapid changes in thermogenic activity, in connection with the known developmental changes in the dependence of the suckling rat's metabolic cold defense on maternal and sibling contact and circadian phase, will distort longitudinal studies of any fast-changing BAT parameter when the conditions immediately before tissue removal are not thoroughly controlled.

scholarly journals Effect of Dietary Docosahexaenoic Acid on In Vitro Thermogenesis and Fatty Acid Compositions of Brown Adipose Tissue.

1998 ◽  
Vol 48 (3) ◽  
pp. 189-196 ◽  
Author(s):  
Hiroshi OHINATA ◽  
Shyamal Kumar SAHA ◽  
Tomie OHNO ◽  
Noriaki HATA ◽  
Yoshihisa MISAWA ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid ◽  
Docosahexaenoic Acid ◽  
Brown Adipose Tissue ◽  
Brown Adipose ◽  
Dietary Docosahexaenoic Acid ◽  
Fatty Acid Compositions

Rat Fetal Brown Adipose Tissue in vitro: Effects of Hormones and Ambient Temperature

Neonatology ◽  
1976 ◽  
Vol 30 (1-4) ◽  
pp. 40-48 ◽  
Author(s):  
J. Frohlich ◽  
P. Hahn ◽  
L. Kirby ◽  
W. Webber
Keyword(s):  
Adipose Tissue ◽  
Ambient Temperature ◽  
Brown Adipose Tissue ◽  
Brown Adipose

scholarly journals Studies on Growth of Brown Adipose Tissue by Means of Cell Culture and Angiogenesis in Vitro Techniques.

1994 ◽  
Vol 48 (6) ◽  
pp. 1058-1066
Author(s):  
Nobumasa UENO ◽  
Ken-ichi NAKAMURA ◽  
Hitoshi YAMASHITA ◽  
Takako KIZAKI ◽  
Tomomi OOKAWARA ◽  
...  
Keyword(s):  
Cell Culture ◽  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
In Vitro Techniques ◽  
Brown Adipose
Sign in / Sign up

Export Citation Format

Share Document