scholarly journals The structure of the human glutathione S-transferase π gene

1988 ◽  
Vol 255 (1) ◽  
pp. 79-83 ◽  
Author(s):  
I G Cowell ◽  
K H Dixon ◽  
S E Pemble ◽  
B Ketterer ◽  
J B Taylor
Keyword(s):  
Phorbol Ester ◽  
Transcription Initiation ◽  
Initiation Site ◽  
Polyoma Virus ◽  
Transcription Initiation Site ◽  
Tata Box ◽  
Cosmid Library ◽  
Sequence Motif ◽  
Glutathione S Transferase ◽  
Responsive Element

The human glutathione S-transferase pi gene has been isolated from a cosmid library. The gene spans approximately 3 kb, is interrupted by six introns and the region around its 5′ end has the high G + C and CpG content typical of an HTF (HpaII tiny fragment) island. In addition to a TATA box at position -28 relative to the transcription initiation site and two G + C boxes (GGGCGG), the promoter contains a sequence motif matching the phorbol ester- and ras-responsive element from the polyoma virus enhancer.

scholarly journals Isolation and characterization of two mouse Pi-class glutathione S-transferase genes

1994 ◽  
Vol 298 (2) ◽  
pp. 385-390 ◽  
Author(s):  
T K Bammler ◽  
C A D Smith ◽  
C R Wolf
Keyword(s):  
Mouse Liver ◽  
Peptide Sequence ◽  
Tata Box ◽  
Sequence Motif ◽  
Glutathione S Transferase ◽  
Coding Regions ◽  
Isolation And Characterization ◽  
Glutathione S Transferases ◽  
Responsive Element

Pi-class glutathione S-transferases (GSTs) play an important role in the detoxification of chemical toxins and mutagens and are implicated in neoplastic development and drug resistance. In all species characterized to date, only one functional Pi-class GST gene has been described. In this report we have identified two actively transcribed murine Pi-class GST genes, Gst p-1 and Gst p-2. The coding regions of Gst p-1 and the mouse Pi-class GST cDNA (GST-II) reported by Hatayama, Satoh and Satoh (1990) (Nucleic Acids Res. 18, 4606) are identical, whereas Gst p-2 encodes a protein that has not been described previously. The two genes are approximately 3 kb long and contain seven exons interrupted by six introns. In addition to a TATA box and a sequence motif matching the phorbol-ester-responsive element, the promoters of Gst p-1 and Gst p-2 exhibit one and two G+C boxes (GGGCGG) respectively. The cDNAs of the two genes were isolated from total liver RNA using reverse PCR. The peptide sequence deduced from the cDNAs share 97% identity and differ in six amino acids. Both genes are transcribed at significantly higher levels in male mouse liver than in female, and Gst p-1 mRNA is more abundant in both sexes than Gst p-2.

Identification of the transcription initiation site of the asexually expressed rRNA genes of the malaria parasite Plasmodium berghei

1999 ◽  
Vol 99 (2) ◽  
pp. 193-205 ◽  
Author(s):  
Rosalina M.L. van Spaendonk ◽  
Glenn A. McConkey ◽  
Jai Ramesar ◽  
Andrei Gabrielian ◽  
Thomas F. McCutchan ◽  
...  
Keyword(s):  
Plasmodium Berghei ◽  
Transcription Initiation ◽  
Malaria Parasite ◽  
Initiation Site ◽  
Transcription Initiation Site ◽  
Rrna Genes ◽  
Parasite Plasmodium
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