Identification of oversulphated galactosaminoglycans in intestinal-mucosal mast cells of rats infected with the nematode worm Nippostrongylus brasiliensis

M Kusche; U Lindahl; L Enerbäck; L Rodén

doi:10.1042/bj2530885

Identification of oversulphated galactosaminoglycans in intestinal-mucosal mast cells of rats infected with the nematode worm Nippostrongylus brasiliensis

Biochemical Journal ◽

10.1042/bj2530885 ◽

1988 ◽

Vol 253 (3) ◽

pp. 885-893 ◽

Cited By ~ 28

Author(s):

M Kusche ◽

U Lindahl ◽

L Enerbäck ◽

L Rodén

Keyword(s):

Mast Cells ◽

Chondroitin Sulphate ◽

Nippostrongylus Brasiliensis ◽

Dermatan Sulphate ◽

Mucosal Mast Cells ◽

Isomeric Structures ◽

Nematode Worm ◽

Type Sequence ◽

Acid Unit

The oversulphated galactosaminoglycans synthesized by rat mucosal mast cells were isolated from the small intestine of animals infected with the nematode Nippostrongylus brasiliensis, which causes proliferation of these cells. The 35S-labelled polysaccharides were degraded by digestion with chondroitinase ABC, and the structures of the disaccharide products were determined by cleavage with mercuric acetate followed by electrophoretic characterization of the resultant sulphated monosaccharides. It was concluded that about half of the disulphated disaccharide units in the polysaccharide consisted of chondroitin sulphate E-type structures [GlcA-GalNAc(4,6-di-OSO3)], in which both sulphate groups were located on the N-acetylgalactosamine unit. The remainder consisted of isomeric structures with one sulphate group on the N-acetylgalactosamine residue and one on the hexuronic acid unit and presumably represented the dermatan sulphate-type sequence [IdoA(2-OSO3)-GalNAc(4-OSO3)].

Download Full-text

Isolation and characterization of ige receptors from rat intestinal mucosal mast cells

European Journal of Immunology ◽

10.1002/eji.1830191019 ◽

1989 ◽

Vol 19 (10) ◽

pp. 1879-1885 ◽

Cited By ~ 5

Author(s):

Mark Swieter ◽

Bosco M. C. Chan ◽

Carol Rimmer ◽

Karol Mcneill ◽

Arnold Froese ◽

...

Keyword(s):

Mast Cells ◽

Isolation And Characterization ◽

Mucosal Mast Cells ◽

Ige Receptors

Download Full-text

Characterization of isolated porcine intestinal mucosal mast cells following infection with Ascaris suum

Veterinary Parasitology ◽

10.1016/0304-4017(88)90122-7 ◽

1988 ◽

Vol 29 (2-3) ◽

pp. 143-158 ◽

Cited By ~ 17

Author(s):

Mohammad Ashraf ◽

Joseph F. Urban ◽

Tin D.G. Lee ◽

Clarence M. Lee

Keyword(s):

Mast Cells ◽

Ascaris Suum ◽

Mucosal Mast Cells

Download Full-text

Murine nippostrongylus brasiliensis (Nb) infection induces changes in intestinal mucosal mast cells (IMMC), IgE levels and afferent nerve activity

Gastroenterology ◽

10.1016/s0016-5085(03)83379-6 ◽

2003 ◽

Vol 124 (4) ◽

pp. A667

Author(s):

Anthony Kirkup ◽

Christine Booth ◽

Andrzej Stanisz ◽

Elaine Fraser ◽

Gervais Tougas ◽

...

Keyword(s):

Mast Cells ◽

Afferent Nerve ◽

Nippostrongylus Brasiliensis ◽

Nerve Activity ◽

Mucosal Mast Cells

Download Full-text

The isolation and characterization of globule leucocytes: their derivation from mucosal mast cells in parasitized sheep

Parasite Immunology ◽

10.1111/j.1365-3024.1984.tb00809.x ◽

1984 ◽

Vol 6 (4) ◽

pp. 371-390 ◽

Cited By ~ 65

Author(s):

J.F. HUNTLEY ◽

G. NEWLANDS ◽

H.R.P. MILLER

Keyword(s):

Mast Cells ◽

Isolation And Characterization ◽

Mucosal Mast Cells

Download Full-text

Characterization of Fcγ receptors on rat mucosal mast cells using a mutant FcεRI-deficient rat basophilic leukemia line

European Journal of Immunology ◽

10.1002/eji.1830251035 ◽

1995 ◽

Vol 25 (10) ◽

pp. 2948-2955 ◽

Cited By ~ 26

Author(s):

Petr Boček ◽

Lubica Dráberová ◽

Petr Dráber ◽

Israel Pecht

Keyword(s):

Mast Cells ◽

Fcγ Receptors ◽

Mucosal Mast Cells ◽

Basophilic Leukemia

Download Full-text

Glycosaminoglycans in rat mucosal mast cells

Biochemical Journal ◽

10.1042/bj2270661 ◽

1985 ◽

Vol 227 (2) ◽

pp. 661-668 ◽

Cited By ~ 83

Author(s):

L Enerbäck ◽

S O Kolset ◽

M Kusche ◽

A Hjerpe ◽

U Lindahl

Keyword(s):

Mast Cells ◽

Glucuronic Acid ◽

Fold Increase ◽

Molar Ratio ◽

Nippostrongylus Brasiliensis ◽

Similar Increase ◽

Selective Labelling ◽

Mucosal Mast Cells ◽

Significant Difference

Rats were infected with the nematode Nippostrongylus brasiliensis, resulting in an approx. 5-fold increase in the number of mucosal mast cells and the histamine content of the intestinal (jejunum) wall. After injection of the infected animals with inorganic [35S]sulphate, a similar increase in the yield of labelled intestinal glycosaminoglycans was observed, compared with uninfected control rats. Autoradiography showed a highly selective labelling of the numerous mucosal mast cells and of the few connective-tissue mast cells in the subserosal region of the bowel. Analysis of the labelled polysaccharide from the infected animals showed that almost 60% of this material consisted of oversulphated galactosaminoglycan, whereas heparin-related polysaccharides accounted for only 13%. The galactosaminoglycan contained 4-monosulphated and 4,6-disulphated N-acetylgalactosamine residues in approx. 5:1 molar ratio, both being linked to D-glucuronic acid residues; the occurrence of L-iduronic acid units could not be excluded. No significant difference in structure was found between this polysaccharide and the corresponding component isolated from uninfected rats. It is concluded that the major polysaccharide produced by rat mucosal mast cells in vivo is an oversulphated galactosaminoglycan rather than heparin.

Download Full-text

Gut mucosal mast cells. Origin, traffic, and differentiation.

Journal of Experimental Medicine ◽

10.1084/jem.160.1.12 ◽

1984 ◽

Vol 160 (1) ◽

pp. 12-28 ◽

Cited By ~ 101

Author(s):

D Guy-Grand ◽

M Dy ◽

G Luffau ◽

P Vassalli

Keyword(s):

T Cell ◽

Mast Cells ◽

Immune Defense ◽

Thoracic Duct Lymph ◽

Blood Levels ◽

Nippostrongylus Brasiliensis ◽

Mesenteric Lymph Nodes ◽

Newborn Mice ◽

Local Factor ◽

Mucosal Mast Cells

Gut mucosal mast cells (MMC), which are nearly absent in normal mice are abundant during nematode infection. In normal mice, study of MMC precursors (MMC-P: cells giving rise to MMC colonies in the presence of IL-3) show that: (a) their frequency, judged by limiting dilution is very high in bone marrow (BM) and gut, and very low in most lymphoid organs and thoracic duct lymph (TDL); (b) gut MMC-P are Thy-1- Lyt-1-2- and are not rapidly replicating; (c) they are the progeny of less differentiated BM MMC-P which are attracted from the blood to the gut mucosa by local factor(s), other than antigen and T cell factors (since normal amounts of gut MMC-P are found in germ-free, nude, and newborn mice). In mice bearing the Wehi 3 tumor (which releases enough IL-3 to produce detectable blood levels) spleen and mesenteric lymph nodes (LN) show increased MMC-P frequency, the greatest increase being in the gut and BM, where numerous differentiated MMC are found. In Nippostrongylus brasiliensis (Nb)-infested mice (known to develop a large, T cell-dependent, gut MMC infiltration), gut MMC-P proliferation is induced by IL-3 released from gut mucosal Thy-1+ Lyt-2- cells, whose in vitro IL-3 release capability is much higher than that of similar cells from normal mice. Both Nb-stimulated T blasts and proliferating MMC-P undergo cyclic traffic, migrating into the TDL and then seeding the whole length of the gut (a process which allows a widespread immune defense after a local antigenic stimulus). Experiments using 2-d interruption of this traffic and fetal gut grafts, suggest that the continuous homing of T blasts back to the gut which leads to permanent Nb-stimulated IL-3 release, is essential for the full maturation of MMC. Transfer experiments in the rat show that TDL circulating MMC-P rapidly mature into MMC when they home back to the Nb-infested gut. It is proposed that gut MMC arise after several stages of progressive differentiation of MMC-P, influenced both by IL-3 and unidentified gut factor(s).

Download Full-text

The glycomes of Caenorhabditis elegans and other model organisms

Biochemical Society Symposium ◽

10.1042/bss0690117 ◽

2002 ◽

Vol 69 ◽

pp. 117-134 ◽

Cited By ~ 47

Author(s):

Stuart M. Haslam ◽

David Gems ◽

Howard R. Morris ◽

Anne Dell

Keyword(s):

Caenorhabditis Elegans ◽

Current Knowledge ◽

Structural Data ◽

Model Organisms ◽

Entire Genome ◽

C Elegans ◽

The Face ◽

Area Of Concern ◽

Nematode Worm

There is no doubt that the immense amount of information that is being generated by the initial sequencing and secondary interrogation of various genomes will change the face of glycobiological research. However, a major area of concern is that detailed structural knowledge of the ultimate products of genes that are identified as being involved in glycoconjugate biosynthesis is still limited. This is illustrated clearly by the nematode worm Caenorhabditis elegans, which was the first multicellular organism to have its entire genome sequenced. To date, only limited structural data on the glycosylated molecules of this organism have been reported. Our laboratory is addressing this problem by performing detailed MS structural characterization of the N-linked glycans of C. elegans; high-mannose structures dominate, with only minor amounts of complex-type structures. Novel, highly fucosylated truncated structures are also present which are difucosylated on the proximal N-acetylglucosamine of the chitobiose core as well as containing unusual Fucα1–2Gal1–2Man as peripheral structures. The implications of these results in terms of the identification of ligands for genomically predicted lectins and potential glycosyltransferases are discussed in this chapter. Current knowledge on the glycomes of other model organisms such as Dictyostelium discoideum, Saccharomyces cerevisiae and Drosophila melanogaster is also discussed briefly.

Download Full-text