scholarly journals Purification of α 2,6-sialyltransferase from rat liver by dye chromatography

1988 ◽  
Vol 253 (2) ◽  
pp. 577-580 ◽  
Author(s):  
U Sticher ◽  
H J Gross ◽  
R Brossmer
Keyword(s):  
Rat Liver ◽  
Cibacron Blue ◽  
Cibacron Blue F3ga

We describe a simple three-step purification for Gal-beta 1,4-GlcNAc-alpha 2,6-sialyltransferase (EC 2.4.99.1) from rat liver which uses chromatography on Cibacron Blue F3GA and f.p.l.c. It gives a highly purified (11,000-fold) enzyme in 19% yield, which is free of other sialyltransferases, CMP-NeuAc hydrolase, sialidases and proteinases.

Inhibition of Rat Liver Asparagine Synthetase by Cibacron Blue F3GA

Enzyme ◽  
1984 ◽  
Vol 31 (3) ◽  
pp. 181-186 ◽  
Author(s):  
Shigeki Hongo ◽  
Tsuneo Sato
Keyword(s):  
Rat Liver ◽  
Asparagine Synthetase ◽  
Cibacron Blue ◽  
Cibacron Blue F3ga

Binding of human interferons to immobilized Cibacron Blue F3GA: the nature of molecular interaction

Biochemistry ◽  
1976 ◽  
Vol 15 (23) ◽  
pp. 5182-5187 ◽  
Author(s):  
Wieslaw J. Jankowski ◽  
Wittiches Von Muenchhausen ◽  
Eugene Sulkowski ◽  
William A. Carter
Keyword(s):  
Molecular Interaction ◽  
Cibacron Blue ◽  
Cibacron Blue F3ga

scholarly journals Kinetic and Thermodynamic Studies of Lysozyme Adsorption on Cibacron Blue F3GA Dye-Ligand Immobilized on Aminated Nanofiber Membrane

Membranes ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 963
Author(s):  
Ai Hsin ◽  
Su-Chun How ◽  
Steven S.-S. Wang ◽  
Chien Wei Ooi ◽  
Chen-Yaw Chiu ◽  
...  
Keyword(s):  
Free Energy ◽  
Free Energy Change ◽  
Energy Change ◽  
Nanofiber Membrane ◽  
Cibacron Blue ◽  
Pseudo Second Order ◽  
Nanofiber Membranes ◽  
Lysozyme Adsorption ◽  
Cibacron Blue F3ga ◽  
Pan Nanofiber

The polyacrylonitrile (PAN) nanofiber membrane was prepared by the electrospinning technique. The nitrile group on the PAN nanofiber surface was oxidized to carboxyl group by alkaline hydrolysis. The carboxylic group on the membrane surface was then converted to dye affinity membrane through reaction with ethylenediamine (EDA) and Cibacron Blue F3GA, sequentially. The adsorption characteristics of lysozyme onto the dye ligand affinity nanofiber membrane (namely P-EDA-Dye) were investigated under various conditions (e.g., adsorption pH, EDA coupling concentration, lysozyme concentration, ionic strength, and temperature). Optimum experimental parameters were determined to be pH 7.5, a coupling concentration of EDA 40 μmol/mL, and an immobilization density of dye 267.19 mg/g membrane. To understand the mechanism of adsorption and possible rate controlling steps, a pseudo first-order, a pseudo second-order, and the Elovich models were first used to describe the experimental kinetic data. Equilibrium isotherms for the adsorption of lysozyme onto P-EDA-Dye nanofiber membrane were determined experimentally in this work. Our kinetic analysis on the adsorption of lysozyme onto P-EDA-Dye nanofiber membranes revealed that the pseudo second-order rate equation was favorable. The experimental data were satisfactorily fitted by the Langmuir isotherm model, and the thermodynamic parameters including the free energy change, enthalpy change, and entropy change of adsorption were also determined accordingly. Our results indicated that the free energy change had a negative value, suggesting that the adsorption process occurred spontaneously. Moreover, after five cycles of reuse, P-EDA-Dye nanofiber membranes still showed promising efficiency of lysozyme adsorption.

The interaction of cibacron blue F3GA with troponin and its subunits

1980 ◽  
Vol 623 (2) ◽  
pp. 243-256 ◽  
Author(s):  
Emil Reisler ◽  
Jane Liu ◽  
Mark Mercola ◽  
Joseph Horwitz
Keyword(s):  
Cibacron Blue ◽  
Cibacron Blue F3ga
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