scholarly journals A comparison of the specificity of phosphatidylcholine synthesis by human fetal lung maintained in either organ or organotypic culture

1988 ◽  
Vol 253 (2) ◽  
pp. 451-457 ◽  
Author(s):  
P A Caesar ◽  
S J Wilson ◽  
C S Normand ◽  
A D Postle
Keyword(s):  
Organotypic Culture ◽  
Fetal Lung ◽  
Reversed Phase ◽  
Lung Cells ◽  
Individual Molecular Species ◽  
Dipalmitoyl Phosphatidylcholine ◽  
Choline Incorporation ◽  
Fractional Synthesis ◽  
Human Fetal Lung ◽  
Phosphatidylcholine Synthesis

Human fetal lung (14-18 weeks gestation) was maintained in either organ or organotypic culture. By 4 days in organ culture or 14 days in organotypic culture, epithelial cells within both culture systems exhibited well-developed apical microvilli and possessed numerous intracellular lamellar bodies characteristic of surfactant phospholipid stores. However, analysis of the pattern of synthesis of individual molecular species of phosphatidylcholine by [14C]choline incorporation and reversed-phase h.p.l.c. showed that this apparent maturation was not paralleled by an increased synthesis of the dipalmitoyl species in either culture system. By contrast, the fractional synthesis of dipalmitoyl phosphatidylcholine, expressed as a percentage of total [14C]choline incorporation, decreased with time in both organ and organotypic culture. Moreover, these fractions were not significantly different from those measured in parallel monolayer cultures of mixed human fetal lung cells that displayed mainly fibroblast morphology. These results suggest that the synthesis pattern of phosphatidylcholine species by lung cells in culture is determined principally by their incubation conditions and not by their state of apparent maturation.

Oxygen modulates surfactant protein mRNA expression and phospholipid production in human fetal lung in vitro

1995 ◽  
Vol 268 (5) ◽  
pp. L818-L825 ◽  
Author(s):  
M. J. Acarregui ◽  
J. J. Brown ◽  
R. K. Mallampalli
Keyword(s):  
Mrna Expression ◽  
Fetal Lung ◽  
Surfactant Protein ◽  
Mrna Levels ◽  
Choline Incorporation ◽  
Associated Proteins ◽  
Dependent Protein ◽  
Surfactant Composition ◽  
Human Fetal Lung

We studied the effect of 20-95% O2 on mRNA levels for the surfactant-associated proteins (SP)-A, SP-B, and SP-C and [3H]choline incorporation into total phosphatidylcholine and type II cell-specific disaturated phosphatidylcholine (DPPC) in human fetal lung in culture. SP-A mRNA levels were increased by 25 and 39% in lung explants incubated in 70 and 95% O2, respectively, compared with levels in tissues incubated in 20% O2. SP-B mRNA levels were unaffected by O2, whereas SP-C mRNA levels were increased by 85, 102, and 115% in atmospheres of 35, 50, and 70% O2, respectively. [3H]choline incorporation into total phosphatidylcholine and DPPC were both increased in human fetal lung explants incubated in increased O2 concentrations compared with tissues incubated in 20% O2. Tissue levels of adenosine 3',5'-cyclic monophosphate (cAMP)-dependent protein kinase (PKA) activity were not affected by O2 concentration, implying that the changes observed in SP mRNA levels and [3H]choline incorporation may not be mediated through alterations in PKA enzyme activity. These findings demonstrate that O2 regulates SP mRNA expression and phospholipid production in human fetal lung in vitro. We speculate that surfactant composition and possibly function may be regulated by O2 in human lung.

An Ultrastructural Comparison of Rat Lung Cells Grown in an Organotypic Culture System with Lung Tissue Found in the Whole Animal

1976 ◽  
Vol 34 ◽  
pp. 172-173
Author(s):  
Peter J. Del Vecchio ◽  
Edward P. Dougherty ◽  
William H. J. Douglas
Keyword(s):  
Lung Tissue ◽  
Organotypic Culture ◽  
Fetal Lung ◽  
Lung Cells ◽  
Type Ii Cells ◽  
Type Ii ◽  
Rat Lung ◽  
Tissue Organization ◽  
Fetal Rat ◽  
Fetal Rat Lung

This study describes the ultrastructure of an organotypic system prepared from rat fetal lung and compares it to lung in rat fetuses. The preparation of the organotypic system has been described elsewhere (1).The organotypic systems in this study are prepared from 18-19 day fetal rat lung and spend two days in culture making the cells a total of 20-21 days old. The fine structure of the type II cells present in this system will be compared to the type II cells in fetal lung at 20-21 days gestation. Because the tissue organization of the organotypic system is more like the organization of fetal lung at an earlier stage (18-19 days gestation) , the cell to cell relationships of the organotypic system are compared to fetal lung tissue at this stage.The histology of the organotypic system is similar to that of the fetal lung in its glandular stage (Fig. 1). The epithelial cells are all columnar and are surrounded by relatively undifferentiated mesenchyme.

The growth promoting effect of cortisol on human fetal lung cells

Steroids ◽  
1973 ◽  
Vol 22 (4) ◽  
pp. 515-524 ◽  
Author(s):  
Barry T. Smith ◽  
John S. Torday ◽  
C.J.P. Giroud
Keyword(s):  
Fetal Lung ◽  
Lung Cells ◽  
Promoting Effect ◽  
Growth Promoting ◽  
Human Fetal Lung
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