scholarly journals Pseudouridine distribution in mammalian 18 S ribosomal RNA. A major cluster in the central region of the molecule

1988 ◽  
Vol 249 (2) ◽  
pp. 459-464 ◽  
Author(s):  
B E H Maden ◽  
J A Wakeman

Human and rodent 18 S rRNA contain about 38 pseudouridine residues. By correlating RNA oligonucleotide data with complete sequence data derived from ribosomal DNA, 30 pseudouridine residues can be located in the RNA sequence, either exactly or to within two or three residues. Pseudouridine and 2′-O-methyl groups are interspersed throughout mammalian 18 S rRNA, but not in closely parallel fashion. Whereas the largest cluster of 2′-O-methyl groups is in the 5′ one-third of the molecule, the greatest concentration of pseudouridine is in the central one-third of the molecule.

Protist ◽  
2012 ◽  
Vol 163 (1) ◽  
pp. 15-24 ◽  
Author(s):  
Marc Gottschling ◽  
Sylvia Soehner ◽  
Carmen Zinssmeister ◽  
Uwe John ◽  
Jörg Plötner ◽  
...  

1989 ◽  
Vol 12 (3) ◽  
pp. 275-279 ◽  
Author(s):  
V.E. Coyne ◽  
C.J. Pillidge ◽  
D.D. Sledjeski ◽  
H. Hori ◽  
B.A. Ortiz-Conde ◽  
...  

Phycologia ◽  
1995 ◽  
Vol 34 (1) ◽  
pp. 65-73 ◽  
Author(s):  
U. Sorhannus ◽  
F. Gasse ◽  
R. Perasso ◽  
A. Baroin Tourancheau

2001 ◽  
Vol 36 (3) ◽  
pp. 275-280 ◽  
Author(s):  
Wolfgang Gross ◽  
Ingo Heilmann ◽  
Dido Lenze ◽  
Claus Schnarrenberger

2018 ◽  
Vol 151 (1) ◽  
pp. 96-109 ◽  
Author(s):  
Ursula Eberhardt ◽  
Nicole Schütz ◽  
Cornelia Krause ◽  
Henry J. Beker

Background and aims – The genus Hebelomina was established in 1935 by Maire to accommodate the new species Hebelomina domardiana, a white-spored mushroom resembling a pale Hebeloma in all aspects other than its spores. Since that time a further five species have been ascribed to the genus and one similar species within the genus Hebeloma. In total, we have studied seventeen collections that have been assigned to these seven species of Hebelomina. We provide a synopsis of the available knowledge on Hebelomina species and Hebelomina-like collections and their taxonomic placement.Methods – Hebelomina-like collections and type collections of Hebelomina species were examined morphologically and molecularly. Ribosomal RNA sequence data were used to clarify the taxonomic placement of species and collections.Key results – Hebelomina is shown to be polyphyletic and members belong to four different genera (Gymnopilus, Hebeloma, Tubaria and incertae sedis), all members of different families and clades. All but one of the species are pigment-deviant forms of normally brown-spored taxa. The type of the genus had been transferred to Hebeloma, and Vesterholt and co-workers proposed that Hebelomina be given status as a subsection of Hebeloma. In the meantime, Hebelomina-like Hebeloma, belonging to seven different species in three different sections, have been found. We conclude that Hebelomina should be abandoned as a supraspecific taxon.


2007 ◽  
Vol 35 (21) ◽  
pp. 7188-7196 ◽  
Author(s):  
E. Pruesse ◽  
C. Quast ◽  
K. Knittel ◽  
B. M. Fuchs ◽  
W. Ludwig ◽  
...  

1985 ◽  
Vol 232 (3) ◽  
pp. 725-733 ◽  
Author(s):  
F S McCallum ◽  
B E H Maden

We have determined the DNA sequences encoding 18 S ribosomal RNA in man and in the frog, Xenopus borealis. We have also corrected the Xenopus laevis 18 S sequence: an A residue follows G-684 in the sequence. These and other available data provide a number of representative examples of variation in primary structure and secondary modification of 18 S ribosomal RNA between different groups of vertebrates. First, Xenopus laevis and Xenopus borealis 18 S ribosomal genes differ from each other by only two base substitutions, and we have found no evidence of intraspecies heterogeneity within the 18 S ribosomal DNA of Xenopus (in contrast to the Xenopus transcribed spacers). Second, the human 18 S sequence differs from that of Xenopus by approx. 6.5%. About 4% of the differences are single base changes; the remainder comprise insertions in the human sequence and other changes affecting several nucleotides. Most of these more extensive changes are clustered in a relatively short region between nucleotides 190 and 280 in the human sequence. Third, the human 18 S sequence differs from non-primate mammalian sequences by only about 1%. Fourth, nearly all of the 47 methyl groups in mammalian 18 S ribosomal RNA can be located in the sequence. The methyl group distribution corresponds closely to that in Xenopus, but there are several extra methyl groups in mammalian 18 S ribosomal RNA. Finally, minor revisions are made to the estimated numbers of pseudouridines in human and Xenopus 18 S ribosomal RNA.


Sign in / Sign up

Export Citation Format

Share Document