scholarly journals The mechanism of action of GTP on Ca2+ efflux from rat liver microsomal vesicles. Measurement of vesicle fusion by fluorescence energy transfer

1988 ◽  
Vol 249 (1) ◽  
pp. 89-93 ◽  
Author(s):  
J G Comerford ◽  
A P Dawson
Keyword(s):  
Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fluorescence Energy Transfer ◽  
Excellent Correlation ◽  
Poly Ethylene Glycol ◽  
Rate Of Increase ◽  
Poly Ethylene ◽  
Fluorescence Energy ◽  
Membrane Probes

1. GTP-promoted fusion between microsomal vesicles was studied by using fluorescence-resonance-energy transfer between the fluorescent membrane probes octadecanoyl-aminofluorescein and octadecyl-rhodamine. 2. The fluorescence increase after GTP addition does not require the presence of ATP, is unaffected by changes in free [Ca2+] in the range 10 microM-1 nM, but requires Mg2+, although higher Mg2+ concentrations are inhibitory. 3. In terms of requirements for poly(ethylene glycol), dependence on GTP concentration and inhibition by high Mg2+ concentrations, there is excellent correlation between rate of increase in fluorescence and rate of GTP-promoted Ca2+ efflux measured under Ca2+ transport conditions. 4. The observations support our previous conclusions that GTP-induced membrane fusion plays a major role in causing GTP-promoted Ca2+ efflux from microsomal vesicles.

A near IR photosensitizer based on self-assembled CdSe quantum dot-aza-BODIPY conjugate coated with poly(ethylene glycol) and folic acid for concurrent fluorescence imaging and photodynamic therapy

RSC Advances ◽  
2016 ◽  
Vol 6 (115) ◽  
pp. 113991-113996 ◽  
Author(s):  
Yu Yan ◽  
Jiangwei Tian ◽  
Fengrui Hu ◽  
Xiaoyong Wang ◽  
Zhen Shen
Keyword(s):  
Photodynamic Therapy ◽  
Folic Acid ◽  
Quantum Dot ◽  
Fluorescence Imaging ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Poly Ethylene Glycol ◽  
Near Ir ◽  
Cdse Quantum Dot ◽  
Poly Ethylene

Nanocomposite comprised of CdSe quantum dot-thiophene-substituted aza-BODIPY conjugate coated with FA and PEG has been developed for simultaneous fluorescence imaging and photodynamic therapy of HeLa cells via Förster resonance energy transfer.

Quantum Dot Bioconjugates as Energy Donors in Fluorescence Resonance Energy Transfer Assays

2003 ◽  
Vol 773 ◽  
Author(s):  
Aaron R. Clapp ◽  
Igor L. Medintz ◽  
J. Matthew Mauro ◽  
Hedi Mattoussi
Keyword(s):  
Energy Transfer ◽  
Quantum Dot ◽  
Organic Dyes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Genetically Engineered ◽  
Molar Ratio ◽  
Binding Assays ◽  
Specific Sequence ◽  
Donor Acceptor

AbstractLuminescent CdSe-ZnS core-shell quantum dot (QD) bioconjugates were used as energy donors in fluorescent resonance energy transfer (FRET) binding assays. The QDs were coated with saturating amounts of genetically engineered maltose binding protein (MBP) using a noncovalent immobilization process, and Cy3 organic dyes covalently attached at a specific sequence to MBP were used as energy acceptor molecules. Energy transfer efficiency was measured as a function of the MBP-Cy3/QD molar ratio for two different donor fluorescence emissions (different QD core sizes). Apparent donor-acceptor distances were determined from these FRET studies, and the measured distances are consistent with QD-protein conjugate dimensions previously determined from structural studies.

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