scholarly journals Liver microsomal membrane fluidity and lipid characteristics in vitamin A-deficient rats

1987 ◽  
Vol 245 (3) ◽  
pp. 907-910 ◽  
Author(s):  
M W Hamm ◽  
V Chan ◽  
G Wolf
Keyword(s):  
Fatty Acids ◽  
Vitamin A ◽  
Membrane Fluidity ◽  
Rat Liver ◽  
Microsomal Fraction ◽  
Liver Microsomes ◽  
Membrane Lipid ◽  
Microsomal Membrane ◽  
Rat Liver Microsomes ◽  
Omega 6

Rat liver microsomes (microsomal fraction) were isolated from vitamin A-deficient and -sufficient rats and analysed for membrane lipid characteristics. Membrane fluidity was found to be significantly decreased in microsomes from the vitamin A-deficient rats, but not in liposomes prepared from lipid extracts. Microsomes from vitamin A-deficient animals showed a significant decrease in C18:2, omega 6 and an increase in C22:5, omega 6 fatty acids.

Membrane lipid modifications: Biosynthesis and identification of in rat liver microsomes

1978 ◽  
Vol 21 (3) ◽  
pp. 175-178 ◽  
Author(s):  
Claudia Moore ◽  
Merle L. Blank ◽  
Ten-Ching Lee ◽  
B. Benjamin ◽  
Claude Piantadosi ◽  
...  
Keyword(s):  
Rat Liver ◽  
Liver Microsomes ◽  
Membrane Lipid ◽  
Rat Liver Microsomes ◽  
Lipid Modifications

Biosynthetic utilization of photoreactive fatty acids by rat liver microsomes

1984 ◽  
Vol 62 (6) ◽  
pp. 375-378 ◽  
Author(s):  
Pierre Leblanc ◽  
Gerhard E. Gerber
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Rat Liver ◽  
Alkyl Chain ◽  
Liver Microsomes ◽  
Rat Liver Microsomes ◽  
Phospholipid Synthesis ◽  
Derivatives Of

The photoreactive ω-diazirinophenoxy derivatives of nonanoate, undecanoate, tridecanoate, and pentadecanoate were shown to be activated by rat liver microsomes to the corresponding acyl-CoA derivatives. The Km and Vmax for these fatty acid analogues were determined; the values obtained indicate that the addition of a photoreactive group to an alkyl chain has an effect similar to that of elongation of the chain by about seven carbons. Incubation of microsomes in the presence of lysophospholipids resulted in the incorporation of the photoreactive fatty acids into the corresponding phospholipids. The ability of mammalian systems to utilize these photoreactive fatty acids for phospholipid synthesis establishes their suitability as photoaffinity analogues of fatty acids.

scholarly journals Activation of a peroxisome-proliferating catabolite of cholic acid to its CoA ester

1993 ◽  
Vol 296 (1) ◽  
pp. 265-270 ◽  
Author(s):  
T Nishimaki-Mogami ◽  
A Takahashi ◽  
Y Hayashi
Keyword(s):  
Cholic Acid ◽  
Rat Liver ◽  
Microsomal Fraction ◽  
Liver Microsomes ◽  
Subcellular Fractionation ◽  
Fatty Acyl ◽  
Peroxisome Proliferator ◽  
Rat Liver Microsomes ◽  
Triton X ◽  
Long Chain

We have shown that a microbial cholic acid catabolite (4R)-4-(2,3,4,6,6a beta,7,8,9,9a alpha,9b beta-decahydro-6a beta-methyl-3-oxo- 1H-cyclopenta[f]quinolin-7 beta-yl)valeric acid (DCQVA), is a potent peroxisome proliferator. In this paper a possible key stage in DCQVA metabolism, the activation of DCQVA to its CoA ester, has been investigated in rat liver microsomes and particulate fractions. The microsomal reaction was dependent on CoA, ATP, DCQVA (0.2-1 mM) and protein content. The reaction was decreased by storage at 4 degrees C, preincubation of microsomes at 37 degrees C for 5 min, or inclusion of Triton X-100 in the reaction mixture. Such treatments also enhanced generation of long-chain fatty acyl-CoAs, as determined by h.p.l.c. analysis. The same effect was caused by exposing the microsomes to phospholipase A2, suggesting that endogenous fatty acids may compete with DCQVA for esterification with CoA. Subcellular fractionation of rat liver demonstrated that the activity of DCQVA-CoA synthesis was localized predominantly in the microsomal fraction, in contrast to long-chain fatty acyl-CoA synthetase, which was distributed among all particulate fractions. Administration of clofibrate of rats did not affect the distribution of DCQVA-CoA synthesis activity. In contrast to a 2-fold induction of long-chain fatty acyl-CoA synthetase by clofibrate treatment, the activity of DCQVA-CoA synthesis in the microsomal fraction decreased by 80%. These results suggest that DCQVA is activated by an enzyme distinct from long-chain fatty acyl-CoA synthetase. The resulting perturbation of fatty acid metabolism may be involved in the mechanism whereby DCQVA causes peroxisome proliferation.

scholarly journals Alteration in Membrane Fluidity of Rat Liver Microsomes and of Liposomes by Protoporphyrin and Its Anti-lipidperoxidative Effect.

2000 ◽  
Vol 23 (4) ◽  
pp. 415-419 ◽  
Author(s):  
Kimie IMAI ◽  
Tachio AIMOTO ◽  
Toshihide SHIMA ◽  
Toshiaki NAKASHIMA ◽  
Masaki SATO ◽  
...  
Keyword(s):  
Membrane Fluidity ◽  
Rat Liver ◽  
Liver Microsomes ◽  
Rat Liver Microsomes
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