scholarly journals Identification of phosphorylated 3-deoxy-manno-octulosonic acid as a component of Haemophilus influenzae lipopolysaccharide

1987 ◽  
Vol 245 (2) ◽  
pp. 583-587 ◽  
Author(s):  
S E Zamze ◽  
M A J Ferguson ◽  
E R Moxon ◽  
R A Dwek ◽  
T W Rademacher
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Alkaline Phosphatase ◽  
Haemophilus Influenzae ◽  
Acid Hydrolysis ◽  
Reliable Method ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Rough Mutant

A phosphorylated 3-deoxy-manno-octulosonic acid (KDO) was released from the lipopolysaccharide (LPS) of the deep rough mutant (Rb+169) of Haemophilus influenzae by acid hydrolysis. Both phosphorylated and dephosphorylated KDO, produced by treatment with alkaline phosphatase, were identified by gas chromatography-mass spectrometry after trimethylsilylation. This technique provides a rapid and reliable method for the identification of phosphorylated KDO in LPS.

scholarly journals Analysis of 5-hydroxytryptamine and its related indoles in cerebrospinal fluid of leukemic children by gas chromatography-mass spectrometry

2020 ◽  
Vol 44 (1) ◽  
pp. 41-45
Author(s):  
Pingping Zhang ◽  
Bo Wang ◽  
Yanmei Sun ◽  
Jian Gao ◽  
Kaoqi Lian
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Cerebrospinal Fluid ◽  
Acetic Acid ◽  
Reliable Method ◽  
Lymphoblastic Leukemia ◽  
Gas Chromatography Mass Spectrometry ◽  
Good Sensitivity ◽  
Chromatography Mass Spectrometry ◽  
Significant Difference

Abstract Background It is meaningful to quantify some neurotransmitters in cerebrospinal fluid (CSF) for pathology and clinical diagnosis. The objective of this study was to develop a rapid and reliable method for detecting 5-hydroxyindole ethanol (5-HTOL), 5-hydroxyindole acetic acid (5-HIAA), 5-hydroxytryptophan (5-HTP) and 5-hydroxytryptamine (5-HT) in CSF by gas chromatography-mass spectrometry (GC-MS), and explore the clinical significance of the levels of these neurotransmitters in CSF from acute lymphoblastic leukemia (ALL). Methods The levels of 5-HTOL, 5-HIAA, 5-HTP and 5-HT in CSF from children with ALL and a control (CON) group were examined by the proposed GC-MS method. Results The GC-MS method showed good sensitivity and accuracy. 5-HT and 5-HIAA contents in the ALL group were significantly lower than those in the CON group (p < 0.01), while there was no significant difference in 5-HTP and 5-HTOL contents between the two groups (p > 0.05). Conclusions This sensitive and reliable method could be used in studies focusing on clinical pathogenesis of ALL.

Development of a reliable method to determine water content by headspace gas chromatography/mass spectrometry with the standard addition technique

2015 ◽  
Vol 7 (11) ◽  
pp. 4816-4820 ◽  
Author(s):  
Shinsuke Inagaki ◽  
Nahoko Morii ◽  
Masahiko Numata
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Water Content ◽  
Reliable Method ◽  
Standard Addition ◽  
Gas Chromatography Mass Spectrometry ◽  
Headspace Gas Chromatography ◽  
Content Determination ◽  
Chromatography Mass Spectrometry ◽  
Headspace Gas

A new and effective method for accurate water content determination using headspace gas chromatography/mass spectrometry (GC/MS) with the standard addition technique was developed.

A simple and reliable method for the determination of nicotine and cotinine in teeth by gas chromatography/mass spectrometry

2003 ◽  
Vol 17 (24) ◽  
pp. 2853-2855 ◽  
Author(s):  
Jos� Antonio Pascual ◽  
David Diaz ◽  
Jordi Segura ◽  
�scar Garcia-Algar ◽  
Oriol Vall ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Reliable Method ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry
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