scholarly journals Properties of the canalicular bile acid transport system in rat liver

1987 ◽  
Vol 242 (2) ◽  
pp. 465-469 ◽  
Author(s):  
P J Meier ◽  
A S Meier-Abt ◽  
J L Boyer
Keyword(s):  
Bile Acid ◽  
Substrate Specificity ◽  
Bile Acids ◽  
Rat Liver ◽  
Transport System ◽  
Acid Transport ◽  
Bile Acid Transport ◽  
Canalicular Bile ◽  
Disulphonic Acid ◽  
Bile Acid Carrier

4,4-Di-isothiocyanostilbene-2,2′-disulphonic acid inhibition of taurocholate efflux from canalicular vesicles was used to demonstrate that potential driven and ‘carrier’-mediated canalicular excretion of taurocholate occur via a common, rather than two separate, pathways. This electrogenic canalicular bile acid ‘carrier ’ preferentially transports trihydroxylated and conjugated dihydroxylated bile acids, but not the unphysiological oxo bile acids, and possibly extends its substrate specificity to other amphipathic molecules such as sulphobromophthalein.

scholarly journals The rat liver ecto-ATPase is also a canalicular bile acid transport protein.

1993 ◽  
Vol 268 (3) ◽  
pp. 2083-2091 ◽  
Author(s):  
C.J. Sippel ◽  
F.J. Suchy ◽  
M. Ananthanarayanan ◽  
D.H. Perlmutter
Keyword(s):  
Bile Acid ◽  
Rat Liver ◽  
Transport Protein ◽  
Acid Transport ◽  
Bile Acid Transport ◽  
Canalicular Bile

scholarly journals Differential Ontogenic Regulation of Basolateral and Canalicular Bile Acid Transport Proteins in Rat Liver

1995 ◽  
Vol 270 (35) ◽  
pp. 20841-20846 ◽  
Author(s):  
Winita Hardikar ◽  
Meenakshisundaram Ananthanarayanan ◽  
Frederick J. Suchy
Keyword(s):  
Bile Acid ◽  
Rat Liver ◽  
Transport Proteins ◽  
Acid Transport ◽  
Bile Acid Transport ◽  
Canalicular Bile

Postnatal expression of the canalicular bile acid transport system of rat liver

1991 ◽  
Vol 260 (5) ◽  
pp. G743-G751 ◽  
Author(s):  
D. A. Novak ◽  
C. J. Sippel ◽  
M. Ananthanarayanan ◽  
F. J. Suchy
Keyword(s):  
Bile Acid ◽  
Rat Liver ◽  
Initial Rate ◽  
Kinetic Studies ◽  
Precipitation Method ◽  
Disulfonic Acid ◽  
Acid Transport ◽  
Adult Rats ◽  
Bile Acid Transport ◽  
Adult Rat

Canalicular plasma membrane (CPM) vesicles prepared by a Ca2+ precipitation method from developing (7 and 14 days old) and adult rat liver were used to directly examine the postnatal ontogenesis of taurocholate (TC) transport. The initial rate of 50 microM TC uptake by vesicles derived from 14-day-old and adult but not 7-day-old animals was markedly inhibited by the anion transport inhibitor 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS). DIDS-sensitive TC uptake was 21.6 +/- 5.6 (SE) at 14 days compared with 58.1 +/- 8.1 pmol.mg protein-1.5 s-1 in adults (P less than or equal to 0.01). Kinetic studies were performed by preloading these predominantly "right-side out" vesicles with TC (25-800 microM) and measuring the initial rate (5 s) of efflux into bile salt-free medium. Computer analysis of the DIDS-sensitive portion of efflux revealed saturable kinetics with a similar Vmax (2.72 +/- 0.36 vs. 1.97 +/- 0.17 nmol.mg protein-1.min-1; P = NS) but a threefold higher Km (0.35 +/- 0.09 vs. 0.11 +/- 0.02 mM; P less than or equal to 0.05) in 14 day vs. adult CPM vesicles. In contrast, efflux from 7 day CPM vesicles increased linearly with increasing concentrations of TC and was not inhibited by DIDS. Immunoblots of canalicular membranes, probed with an antibody against the 100-kDa bile acid transport protein, showed that the amount of immunoreactive carrier protein in the membranes of 14-day-old and adult rats was similar but was only 37% of the adult level at 7 days of age.(ABSTRACT TRUNCATED AT 250 WORDS)

Effect of thiazolidinediones on bile acid transport in rat liver

Life Sciences ◽  
2007 ◽  
Vol 80 (8) ◽  
pp. 732-740 ◽  
Author(s):  
Kris L. Snow ◽  
Richard H. Moseley
Keyword(s):  
Bile Acid ◽  
Rat Liver ◽  
Acid Transport ◽  
Bile Acid Transport

Effect of endotoxin on bile acid transport in rat liver: a potential model for sepsis-associated cholestasis

1996 ◽  
Vol 271 (1) ◽  
pp. G137-G146 ◽  
Author(s):  
R. H. Moseley ◽  
W. Wang ◽  
H. Takeda ◽  
K. Lown ◽  
L. Shick ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Bile Acid ◽  
Rat Liver ◽  
Membrane Vesicles ◽  
Tnf Alpha ◽  
Mrna Levels ◽  
Acid Transport ◽  
Plasma Membrane Vesicles ◽  
Bile Acid Transport ◽  
Taurocholate Transport

Intrahepatic cholestasis in the setting of extrahepatic bacterial infection has been attributed to the effects of endotoxin and cytokines such as tumor necrosis factor-alpha (TNF-alpha) on bile acid transport. To define the mechanism of sepsis-associated cholestasis, taurocholate transport was examined in basolateral (bLPM) and canalicular (cLPM) rat liver plasma membrane vesicles derived from control and endotoxin [lipopolysaccharide (LPS)]-treated animals and in plasma membrane vesicles prepared after TNF-alpha treatment. Na(+)-dependent [3H]taurocholate uptake and both membrane-potential-dependent and ATP-dependent [3H]taurocholate transport were reduced in bLPM and cLPM vesicles, respectively, after LPS treatment. In membrane vesicles from TNF-alpha-treated animals, Na(+)-dependent [3H]taurocholate uptake was also reduced. Northern blot hybridization, using cDNA probes for the putative sinusoidal bile acid transporter (Ntcp) and canalicular ecto-adenosinetriphosphatase, demonstrated decreased mRNA levels after LPS and TNF-alpha treatment. Immunoblot analysis of membrane extracts from LPS-treated animals revealed decreased levels of these putative bile acid transporters. Impaired bile acid transport at the sinusoidal and canalicular membrane domains by these and other mediators of the inflammatory response may account for sepsis-associated cholestasis.

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