scholarly journals Changes in glucose 1,6-bisphosphate content in rat skeletal muscle during contraction

1986 ◽  
Vol 240 (3) ◽  
pp. 747-751 ◽  
Author(s):  
A M Bassols ◽  
J Carreras ◽  
R Cussó
Keyword(s):  
Skeletal Muscle ◽  
Sciatic Nerve ◽  
Muscle Contraction ◽  
Gastrocnemius Muscle ◽  
Lactate Concentration ◽  
Glycolytic Flux ◽  
Rat Skeletal Muscle ◽  
Contracting Muscle ◽  
Apparent Relationship

Glucose 1,6-bisphosphate, fructose 2,6-bisphosphate, glycogen, lactate and other glycolytic metabolites were measured in rat gastrocnemius muscle, which was electrically stimulated in situ via the sciatic nerve. Both the frequency and the duration of stimulation were varied to obtain different rates of glycolysis. There was no apparent relationship between fructose 2,6-bisphosphate content and lactate accumulation in contracting muscle. In contrast, glucose 1,6-bisphosphate content increased with lactate concentration during contraction. It is suggested that the increase in glucose 1,6-bisphosphate could play a role in phosphofructokinase stimulation and in the activation of the glycolytic flux during muscle contraction.

scholarly journals Effect of Qianjinjiu on the Contraction of Gastrocnemius muscle of Isolated Sciatic Nerve

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Jianqing Zhu ◽  
Zhanying Qi ◽  
Chaohui Lin
Keyword(s):  
Skeletal Muscle ◽  
Sciatic Nerve ◽  
Muscle Contraction ◽  
Gastrocnemius Muscle ◽  
Treatment Time ◽  
Salt Water ◽  
Water Extract ◽  
Treatment Level ◽  
Muscle Contractility ◽  
Time Point

Objective: To investigate the water extract from Radix (Flemingia philippinensis Merr et Rolfe Decoction.) Effect of sciatic nerve gastrocnemius muscle Characteristics of isolated frog. Methods: The dissected lesion of sciatic nerve gastrocnemius muscle was infused with 0.9% saline, 0.125g / mL, 0.25g / mL, 0.5g / mL jelly extract, and the RM6240 bio-signal collector was used to measure each The infiltration depth of 5 minutes, infiltration for 10 minutes, infiltration of 15 minutes at each time point of the gastrocnemius muscle contraction threshold strength (measured at each time point 5 times) and complete single contraction curve (measured at each time point 25) Systolic rate, systolic rate, rate of contraction, and latency. Results: The systolic threshold intensity of the gastrocnemius muscle increased with the increase of the concentration of the extraction fluid, but less than the pre-treatment level (P <0.01, P <0.05). If the concentration and time (P <0.05). At the same time, the systolic amplitude, systolic rate and diastolic rate increased first and then decreased with the increase of the concentration of the extract (P <0.01, P <0.05). The latency of the sciatic nerve of the sciatic nerve was affected by the concentration of the drug in the infiltration solution, which decreased first and then increased with the increase of the drug concentration. Conclusion: The systolic characteristics of skeletal muscle are affected by the weight of the components. Low concentration of salt water extract in a short time to improve the ability of skeletal muscle contraction, treatment time is too long or too high concentration of crude drugs will make the skeletal muscle contractility weakened, and even inhibit the skeletal muscle contractility, and affect the nerve Muscle excitement signal transmission.

Contraction duration affects metabolic energy cost and fatigue in skeletal muscle

1998 ◽  
Vol 274 (3) ◽  
pp. E397-E402 ◽  
Author(s):  
Michael C. Hogan ◽  
Erica Ingham ◽  
S. Sadi Kurdak
Keyword(s):  
Skeletal Muscle ◽  
Short Duration ◽  
Energy Cost ◽  
Lactate Concentration ◽  
Metabolic Energy ◽  
Muscle Lactate ◽  
Contracting Muscle ◽  
Contraction Duration ◽  
Long Duration ◽  
The Difference

It has been suggested that during a skeletal muscle contraction the metabolic energy cost at the onset may be greater than the energy cost related to holding steady-state force. The purpose of the present study was to investigate the effect of contraction duration on the metabolic energy cost and fatigue process in fully perfused contracting muscle in situ. Canine gastrocnemius muscle ( n = 6) was isolated, and two contractile periods (3 min of isometric, tetanic contractions with 45-min rest between) were conducted by each muscle in a balanced order design. The two contractile periods had stimulation patterns that resulted in a 1:3 contraction-to-rest ratio, with the difference in the two contractile periods being in the duration of each contraction: short duration 0.25-s stimulation/0.75-s rest vs. long duration 1-s stimulation/3-s rest. These stimulation patterns resulted in the same total time of stimulation, number of stimulation pulses, and total time in contraction for each 3-min period. Muscle O2 uptake, the fall in developed force (fatigue), the O2 cost of developed force, and the estimated total energy cost (ATP utilization) of developed force were significantly greater ( P < 0.05) with contractions of short duration. Lactate efflux from the working muscle and muscle lactate concentration were significantly greater with contractions of short duration, such that the calculated energy derived from glycolysis was three times greater in this condition. These results demonstrate that contraction duration can significantly affect both the aerobic and anaerobic metabolic energy cost and fatigue in contracting muscle. In addition, it is likely that the greater rate of fatigue with more rapid contractions was a result of elevated glycolytic production of lactic acid.

Modulation effects of cordycepin on the skeletal muscle contraction of toad gastrocnemius muscle

2014 ◽  
Vol 726 ◽  
pp. 9-15 ◽  
Author(s):  
Li-Hua Yao ◽  
Wei Meng ◽  
Rong-Feng Song ◽  
Qiu-Ping Xiong ◽  
Wei Sun ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Contraction ◽  
Gastrocnemius Muscle ◽  
Skeletal Muscle Contraction

Length dependence of staircase potentiation: interactions with caffeine and dantrolene sodium

2000 ◽  
Vol 78 (4) ◽  
pp. 350-357 ◽  
Author(s):  
Dilson E Rassier ◽  
Brian R MacIntosh
Keyword(s):  
Skeletal Muscle ◽  
Gastrocnemius Muscle ◽  
Repetitive Stimulation ◽  
Mammalian Skeletal Muscle ◽  
Optimal Length ◽  
Dantrolene Sodium ◽  
Length Dependence ◽  
Before And After ◽  
Isometric Twitch

In skeletal muscle, there is a length dependence of staircase potentiation for which the mechanism is unclear. In this study we tested the hypothesis that abolition of this length dependence by caffeine is effected by a mechanism independent of enhanced Ca2+ release. To test this hypothesis we have used caffeine, which abolishes length dependence of potentiation, and dantrolene sodium, which inhibits Ca2+ release. In situ isometric twitch contractions of rat gastrocnemius muscle before and after 20 s of repetitive stimulation at 5 Hz were analyzed at optimal length (Lo), Lo - 10%, and Lo + 10%. Potentiation was observed to be length dependent, with an increase in developed tension (DT) of 78 ± 12, 51 ± 5, and 34 ± 9% (mean ± SEM), at Lo - 10%, Lo, and Lo + 10%, respectively. Caffeine diminished the length dependence of activation and suppressed the length dependence of staircase potentiation, giving increases in DT of 65±13, 53 ± 11, and 45 ± 12% for Lo - 10%, Lo, and Lo + 10%, respectively. Dantrolene administered after caffeine did not reverse this effect. Dantrolene alone depressed the potentiation response, but did not affect the length dependence of staircase potentiation, with increases in DT of 58 ± 17, 26 ± 8, and 18 ± 7%, respectively. This study confirms that there is a length dependence of staircase potentiation in mammalian skeletal muscle which is suppressed by caffeine. Since dantrolene did not alter this suppression of the length dependence of potentiation by caffeine, it is apparently not directly modulated by Ca2+ availability in the myoplasm.

Metabolic intermediates and lactate diffusion in active dog skeletal muscle

1976 ◽  
Vol 231 (3) ◽  
pp. 766-771 ◽  
Author(s):  
TE Graham ◽  
DG Sinclair ◽  
CK Chapler
Keyword(s):  
Skeletal Muscle ◽  
Gastrocnemius Muscle ◽  
Lactate Concentration ◽  
Arterial Occlusion ◽  
Fold Increase ◽  
Major Influence ◽  
Muscle Lactate ◽  
Metabolic Intermediates ◽  
Anesthetized Dogs ◽  
Resting Muscle

The concentration of several metabolic intermediates, blood flow (Q), oxygen uptake (VO2), and lactate release (La) were measured in the gastrocnemius muscle of anesthetized dogs. Muscle lactate concentration increased from 1.6 to 2.7 mumol/g wet wt (P less than 0.05) during 0.5-5 min of contractions at 5 twitches/s but was not different from the contralateral resting muscle at 15, 30, or 60 min. Glycerophosphate increased from 0.35 to 0.70 mumol/g wet wt (P less than 0.05) during 0.5-5 min of activity, whereas muscle pyruvate decreased from 0.09 to 0.07 mumol/g wet wt (P less than 0.05). The concentration of NAD did not change in 9 of 11 experiments during contractions, despite a 12- to 15-fold increase in La. Significant decreases in NAD were observed when Q was compromised by arterial occlusion during contractions. No demonstrable relationship existed between La and either the muscle lactate concentration or muscle-venous lactate concentration gradient. Q was positively correlated with both La and muscle lactate during the first 5-15 min of activity. We conclude that increased La or increased lactate concentration in muscle need not be associated with hypoxia and that Q has a major influence on La.

Effect of respiratory alkalosis on skeletal muscle metabolism in the dog

1985 ◽  
Vol 58 (2) ◽  
pp. 658-664 ◽  
Author(s):  
A. G. Brice ◽  
H. G. Welch
Keyword(s):  
Skeletal Muscle ◽  
Hind Limb ◽  
Gastrocnemius Muscle ◽  
Minute Ventilation ◽  
Lactate Concentration ◽  
Muscle Metabolism ◽  
Respiratory Alkalosis ◽  
Whole Body ◽  
Venous Blood Flow ◽  
Skeletal Muscle Metabolism

These experiments were conducted to determine whether changes in skeletal muscle metabolism contribute to the previously reported increase in whole-body O2 uptake (VO2) during respiratory alkalosis. The hind-limb and gastrocnemius-plantaris preparations in anesthetized and paralyzed dogs were used. VO2 of the hindlimb and gastrocnemius muscle was calculated from measurements of venous blood flow and arterial and venous O2 concentrations (Van Slyke analysis). Whole-body VO2 was measured by the open-circuit method. Minute ventilation (hence blood gases and pH) was controlled by a mechanical respirator. Whole-body, hind-limb, and gastrocnemius muscle VO2 increased 14, 19, and 20%, respectively, during alkalosis (P less than 0.05). In all experiments, arterial lactate concentration increased significantly (P less than 0.05) during alkalosis. A positive venoarterial lactate difference across muscle during alkalosis indicated that skeletal muscle is a source of the elevated blood lactate. We concluded that VO2 of resting skeletal muscle is increased during states of respiratory alkalosis and that this increase can account for much of the increase in whole-body VO2.

Enhanced arteriolar α-adrenergic constriction impairs dilator responses and skeletal muscle perfusion in obese Zucker rats

2004 ◽  
Vol 97 (2) ◽  
pp. 764-772 ◽  
Author(s):  
Jefferson C. Frisbee
Keyword(s):  
Skeletal Muscle ◽  
Blood Flow ◽  
Gastrocnemius Muscle ◽  
Muscle Blood Flow ◽  
Zucker Rats ◽  
Metabolic Demand ◽  
Obese Zucker Rats ◽  
Isometric Twitch ◽  
Gastrocnemius Muscles

The present study tested the hypothesis that enhanced vascular α-adrenergic constriction in obese Zucker rats (OZR) impairs arteriolar dilation and perfusion of skeletal muscle at rest and with increased metabolic demand. In lean Zucker rats (LZR) and OZR, isolated gracilis arterioles were viewed via television microscopy, and the contralateral cremaster muscle or gastrocnemius muscle was prepared for study in situ. Gracilis and cremasteric arterioles were challenged with dilator stimuli under control conditions and after blockade of α-adrenoreceptors with prazosin, phentolamine, or yohimbine. Gastrocnemius muscles performed isometric twitch contractions of increasing frequency, and perfusion was continuously monitored. In OZR, dilator responses of arterioles to hypoxia (gracilis), wall shear rate (cremaster), acetylcholine, and iloprost (both) were impaired vs. LZR. Treatment with prazosin and phentolamine (and in cremasteric arterioles only, yohimbine) improved arteriolar reactivity to these stimuli in OZR, although responses remained impaired vs. LZR. Gastrocnemius muscle blood flow was reduced at rest in OZR; this was corrected with intravenous infusion of phentolamine or prazosin. At all contraction frequencies, blood flow was reduced in OZR vs. LZR; this was improved by infusion of phentolamine or prazosin at low-moderate metabolic demand only (1 and 3 Hz). At 5 Hz, adrenoreceptor blockade did not alter blood flow in OZR from levels in untreated rats. These results suggest that enhanced α-adrenergic constriction of arterioles of OZR contributes to impaired dilator responses and reduced muscle blood flow at rest and with mild-moderate (although not with large) elevations in metabolic demand.

scholarly journals Electrical characteristics of rat skeletal muscle in immaturity, adulthood and after sciatic nerve injury, and their relation to muscle fiber size

2009 ◽  
Vol 30 (12) ◽  
pp. 1415-1427 ◽  
Author(s):  
Mohammad A Ahad ◽  
P Michelle Fogerson ◽  
Glenn D Rosen ◽  
Pushpa Narayanaswami ◽  
Seward B Rutkove
Keyword(s):  
Skeletal Muscle ◽  
Sciatic Nerve ◽  
Nerve Injury ◽  
Muscle Fiber ◽  
Sciatic Nerve Injury ◽  
Electrical Characteristics ◽  
Rat Skeletal Muscle ◽  
Fiber Size

Bradykinin facilitates noradrenaline spillover during contraction in the canine gracilis muscle

2001 ◽  
Vol 79 (10) ◽  
pp. 831-835
Author(s):  
Julie L Lavoie ◽  
Louise Béliveau
Keyword(s):  
Skeletal Muscle ◽  
Muscle Contraction ◽  
Gracilis Muscle ◽  
Bradykinin Receptor ◽  
Hoe 140 ◽  
Underlying Mechanisms

Noradrenaline spillover from skeletal muscle vascular areas increases during exercise but the underlying mechanisms are not well understood. Muscle contraction itself causes changes in many factors that could affect noradrenaline spillover. For instance, it has been reported that bradykinin is synthesized in skeletal muscle areas during contraction. Because the B2 bradykinin receptor facilitates noradrenaline spillover, it may be involved in the increase associated with contraction. In this experiment, we studied the effect of bradykinin on noradrenaline spillover in the in situ canine gracilis muscle, using the specific B2 antagonist HOE 140. The drug did not modify noradrenaline spillover at rest, but did cause a significant decrease during muscle contraction, from 558 to 181 pg·min–1. As reported previously in the literature, fractional extraction of noradrenaline decreased during muscle contraction. This effect was independent of HOE 140 treatment. In light of our results, it seems that bradykinin formation during muscle contraction may play an important part in the observed increase in noradrenaline spillover but does not affect fractional extraction.Key words: skeletal muscle, fractional extraction, stimulation, HOE 140, B2 receptors.

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