scholarly journals Characteristics of rat liver microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase

1986 ◽  
Vol 233 (1) ◽  
pp. 167-172 ◽  
Author(s):  
G C Ness ◽  
C E Sample ◽  
M Smith ◽  
L C Pendleton ◽  
D C Eichler
Keyword(s):  
Rat Liver ◽  
Coenzyme A ◽  
Rabbit Antiserum ◽  
Single Band ◽  
Coa Reductase ◽  
Coenzyme A Reductase

A procedure for the preparation of rat liver microsomal fractions essentially devoid of contaminating lysosomes is described. When this preparation was examined by immunoblotting with a rabbit antiserum to rat 3-hydroxy-3-methylglutaryl-CoA reductase, a single band corresponding to an Mr of 100000 was observed. No evidence was found for glycosylation of rat liver-3-hydroxy-3-methylglutaryl-CoA reductase. Native rat liver microsomal 3-hydroxy-3-methylglutaryl-CoA reductase differs from the purified proteolytically modified species in that it displays allosteric kinetics towards NADPH.

scholarly journals A new method for assaying rat liver microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity and its application in a study of the effect of dietary cholesterol on this effect of dietary cholesterol on this enzyme

1977 ◽  
Vol 164 (3) ◽  
pp. 501-508 ◽  
Author(s):  
Y A Baqir ◽  
R Booth
Keyword(s):  
Rat Liver ◽  
Cholesterol Ester ◽  
Time Course ◽  
Coenzyme A ◽  
Reductase Activity ◽  
Dietary Cholesterol ◽  
New Method ◽  
Coa Reductase ◽  
Microsomal Cholesterol ◽  
Coenzyme A Reductase

A new method suitable for measuring rat liver 3-hydroxy-3-methylglutaryl-CoA reductase activity is described and its advantages over methods previously available are discussed. An accurate time course was measured for the inhibition of liver microsomal 3-hydroxy-3-methylglutaryl-CoA reductase activity by dietary cholesterol; this enzyme was affected 1 1/4 h after the rats began to consume a cholesterol-rich diet. In this experiment there was no correlation between concentrations of microsomal cholesterol ester and the activity of 3-hydroxy-3-methylglutary-CoA reductase.

scholarly journals Specificity of the effect of dietary cholesterol on rat liver microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase activity (Short Communication)

1975 ◽  
Vol 148 (2) ◽  
pp. 337-339
Author(s):  
Keith W. Gregory ◽  
Roger Booth
Keyword(s):  
Rat Liver ◽  
Short Communication ◽  
Coenzyme A ◽  
Regulatory Mechanism ◽  
Reductase Activity ◽  
Dietary Cholesterol ◽  
Microsomal Enzymes ◽  
Coa Reductase ◽  
Liver Microsomal Enzymes ◽  
Coenzyme A Reductase

Dietary cholesterol lowers the activity of rat liver microsomal 3-hydroxy-3-methylglutaryl-CoA reductase without affecting various other liver microsomal enzymes. This is consistent with a specific regulatory mechanism and distinguishes the action of cholesterol on 3-hydroxy-3-methylglutaryl-CoA reductase from that of at least one other stimulus known to affect this enzyme.

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