scholarly journals Control of glycogen phosphorylase interconversion by phorbol esters, diacylglycerols, Ca2+ and hormones in isolated rat hepatocytes

1985 ◽  
Vol 231 (3) ◽  
pp. 511-516 ◽  
Author(s):  
G van de Werve ◽  
J Proietto ◽  
B Jeanrenaud
Keyword(s):  
Glycogen Phosphorylase ◽  
Phorbol Esters ◽  
Rat Hepatocytes ◽  
Ionophore A23187 ◽  
Dose Dependency ◽  
Isolated Rat Hepatocytes ◽  
Kinase C ◽  
Similar Dose ◽  
Adrenergic Antagonist ◽  
Isolated Rat

In isolated rat hepatocytes: phosphorylase activation by the ionophore A23187 was enhanced in the presence of tumour-promoting phorbol esters and 1,2- (but not 1,3-) diacylglycerols (dioleoyl- and oleoylacetyl-glycerol), with a similar dose-dependency; the activation of phosphorylase by phenylephrine (1 microM) (but not by vasopressin or glucagon) was inhibited both by tumour-promoting phorbol esters and diacylglycerols, but with a different dose-dependency: complete inhibition was achieved with concentrations of phorbol esters two orders of magnitude lower than those of diacylglycerol; binding of the alpha 1-adrenergic antagonist [3H]prazosin and its displacement by unlabelled prazosin was not significantly affected in the presence of the phorbol esters. The possible involvement of protein kinase C in the control of phosphorylase interconversion is discussed.

scholarly journals Effect of pertussis toxin and phorbol esters on α1B-adrenoceptor-mediated second messenger responses in isolated rat hepatocytes.

1993 ◽  
Vol 61 ◽  
pp. 158
Author(s):  
Haruhito Kondo ◽  
Takahide Nomura ◽  
Seiko Hasegawa ◽  
Toshiko Watanabe ◽  
Rie Yokoyama ◽  
...  
Keyword(s):  
Pertussis Toxin ◽  
Phorbol Esters ◽  
Second Messenger ◽  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat

Carbon tetrachloride-induced inhibition of protein kinase C in isolated rat hepatocytes

1988 ◽  
Vol 153 (2) ◽  
pp. 591-597 ◽  
Author(s):  
Giuseppe Poli ◽  
Emanuele Albano ◽  
Mario U. Dianzani ◽  
Edon Melloni ◽  
Sandro Pontremoli ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Carbon Tetrachloride ◽  
Protein Kinase ◽  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Kinase C ◽  
Isolated Rat

Decreased protein kinase C activity is associated with programmed cell death (apoptosis) in freshly isolated rat hepatocytes

1992 ◽  
Vol 12 (3) ◽  
pp. 199-206 ◽  
Author(s):  
Victor Sanchez ◽  
Miguel Lucas ◽  
Aureo Sanz ◽  
Raimundo Goberna
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Time Course ◽  
Rat Hepatocytes ◽  
Polymyxin B ◽  
Isolated Rat Hepatocytes ◽  
Protein Kinase C Inhibitors ◽  
Kinase C ◽  
Freshly Isolated Rat Hepatocytes ◽  
Isolated Rat

Apoptosis of freshly isolated rat hepatocytes was induced by either the omission of fetal bovine serum in the culture medium or addition of the protein kinase C inhibitors polymyxin B or staurosporin. The time-course of DNA breakdown into oligonucleosome-sized fragments and the activity of protein kinase C was determined. Hepatocytes were found to be sensitive to bleomycin which induced a high degree of DNA breakdown even within 30 min incubation. Both staurosporin and polymyxin B induced DNA degradation in hepatocytes after three hours incubation, an effect that was partially prevented by phorbol myristate acetate (PMA). After eight hours incubation, PMA failed to counteract this action and itself produced the apoptosis of rat hepatocytes. The results suggest the involvement of protein kinase C in hepatocyte survival.

scholarly journals Stimulatory effect of the intestinal peptide PHI on glycogenolysis and gluconeogenesis in isolated rat hepatocytes

1983 ◽  
Vol 214 (3) ◽  
pp. 999-1002 ◽  
Author(s):  
J E Felíu ◽  
J Marco
Keyword(s):  
Cyclic Amp ◽  
Pyruvate Kinase ◽  
Glycogen Phosphorylase ◽  
Rat Hepatocytes ◽  
Fold Increase ◽  
Isolated Rat Hepatocytes ◽  
Mediated Effects ◽  
Dose Dependent ◽  
Isolated Rat ◽  
Stimulation Of

The newly isolated peptide PHI provoked a dose-dependent stimulation of glycogenolysis and gluconeogenesis in isolated rat hepatocytes; at 1 microM-PHI, both processes were increased 1.6-fold as compared with basal values. These PHI-mediated effects were accompanied by the activation of glycogen phosphorylase and the inactivation of pyruvate kinase. PHI (1 microM) also caused a 2-fold increase in hepatocyte cyclic AMP.

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