scholarly journals Intercellular pathway of leucine catabolism in rat spermatogenic epithelium

1985 ◽  
Vol 228 (3) ◽  
pp. 775-775
Keyword(s):  
Leucine Catabolism ◽  
Intercellular Pathway ◽  
Spermatogenic Epithelium

scholarly journals Intercellular pathway of leucine catabolism in rat spermatogenic epithelium

1985 ◽  
Vol 226 (3) ◽  
pp. 889-892 ◽  
Author(s):  
J A Grootegoed ◽  
R Jansen ◽  
H J van der Molen
Keyword(s):  
Lactate Dehydrogenase ◽  
Germ Cell ◽  
Sertoli Cells ◽  
Male Germ Cell ◽  
Spermatogenic Cells ◽  
Dehydrogenase Isoenzyme ◽  
Leucine Catabolism ◽  
Intercellular Pathway ◽  
Spermatogenic Epithelium

A unique intercellular pathway of leucine catabolism was observed in vitro in rat spermatogenic epithelium. Sertoli cells convert leucine via transmination into 4-methyl-2-oxovalerate, and spermatocytes and spermatids reduce exogenous 4-methyl-2-oxovalerate to 2-hydroxy-4-methylvalerate, which is then released by the spermatogenic cells. The NADH-dependent reduction of 4-methyl-2-oxovalerate could be catalysed by the male-germ-cell-specific lactate dehydrogenase isoenzyme LDH-C4 in the cytosol of the spermatogenic cells, concomitant with the NAD+-dependent conversion of exogenous lactate into pyruvate.

Gas-chromatographic/mass spectrometric detection of 3-hydroxy-3-methylglutaryl-CoA lyase deficiency in double first cousins.

1982 ◽  
Vol 28 (1) ◽  
pp. 137-140 ◽  
Author(s):  
E J Norman ◽  
M D Denton ◽  
H K Berry
Keyword(s):  
Gas Chromatography Mass Spectrometry ◽  
Fat Intake ◽  
Acid Excretion ◽  
Reye's Syndrome ◽  
Low Fat Diet ◽  
Low Protein ◽  
Urinary Organic Acids ◽  
Life Threatening ◽  
Leucine Catabolism ◽  
Chromatographic Mass

Abstract Gas chromatography/mass spectrometry was used for the detection of 3-hydroxy-3-methylglutaryl-CoA lyase (EC 4.1.3.4) deficiency in double first cousins. This enzyme is in the last step of leucine catabolism and is also involved in ketogenesis. Quantitation of urinary organic acids as their cyclohexyl esters demonstrated increased concentrations of 3-hydroxy-3-methylglutaric acid, 3-methylglutaconic acid, 3-methylglutaric acid, and 3-hydroxyisovaleric acid. The procedure is more rapid, sensitive, and specific than previously reported gas-chromatographic methods for acid quantitation. The affected children initially presented with symptoms similar to Reye's syndrome; the acids were quantitated during periods of altered intake of protein and fat. Both leucine and fat intake contributed to increased acid excretion. These studies suggest that life-threatening episodes of hypoglycemia are best prevented with a low-protein, low-fat diet.

scholarly journals Correlation between testicular mast cell count and spermatogenic epithelium in non-obstructive azoospermia

2018 ◽  
Vol 99 (1) ◽  
pp. 22-28 ◽  
Author(s):  
Ahmed A. M. Abdel-Hamid ◽  
Hoda Atef ◽  
Khaled R. Zalata ◽  
Atef Abdel-Latif
Keyword(s):  
Mast Cell ◽  
Cell Count ◽  
Obstructive Azoospermia ◽  
Mast Cell Count ◽  
Spermatogenic Epithelium

Effects of a New Benzimidazole Derivative with Actoprotective Properties on Spermatogenic Epithelium of the Testes and Fertility of Male White Rats

2020 ◽  
Vol 169 (1) ◽  
pp. 57-59
Author(s):  
L. I. Bugaeva ◽  
E. A. Kuzubova ◽  
V. I. Petrov ◽  
V. A. Kataev ◽  
I. V. Kharlamov ◽  
...  
Keyword(s):  
Benzimidazole Derivative ◽  
White Rats ◽  
Spermatogenic Epithelium

Relationship between testicular morphology and sperm production following ischaemia in the ram

1995 ◽  
Vol 7 (1) ◽  
pp. 119 ◽  
Author(s):  
CM Markey ◽  
AM Jequier ◽  
GT Meyer ◽  
GB Martin
Keyword(s):  
Morphological Changes ◽  
Sperm Concentration ◽  
Testicular Biopsy ◽  
Seminiferous Tubules ◽  
Testis Weight ◽  
Infertile Men ◽  
Testicular Morphology ◽  
And Function ◽  
The Relationship ◽  
Spermatogenic Epithelium

Arteriosclerosis was induced in the internal spermatic artery of rams to determine if this condition is implicated in the aetiology of testicular pathology which causes male infertility. Data were collected on sperm concentration and motility for 56 days following surgery to provide an index of testicular function. Testes were then weighed and a testicular biopsy score count was performed on histological sections to assess spermatogenic potential of seminiferous tubules. Vascular disturbance caused focal damage of the seminiferous epithelium, similar to that seen among infertile men, and a reduction in ejaculate volume, sperm concentration and sperm motility. Sperm concentration decreased following ischaemia yet was maintained to some degree by a germ-cell depleted spermatogenic epithelium. Normal testicular morphology was maintained above a testis weight of about 120 g (for an individual testis), but below this threshold spermatogenesis was severely impaired. In conclusion, these data have provided information on the relationship between testicular morphology and function following ischaemia in the ram. Furthermore, the morphological changes induced in the testis were similar to those seen among infertile men and, by their focal nature, could explain the distinction between oligozoospermia and azoospermia in men exhibiting spermatogenic arrest.

Caryopathic effect of bio-preparation Bacillus subtilis 12В on the status of spermatogenic epithelium of animals infected with helminths

2016 ◽  
Vol 3 (1) ◽  
pp. 91-97
Author(s):  
Сивкова ◽  
T. Sivkova ◽  
Красникова ◽  
E. Krasnikova ◽  
Шураков ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Mitotic Index ◽  
Fasciola Hepatica ◽  
White Male ◽  
Joint Effect ◽  
Negative Effects ◽  
Protein Extract ◽  
The Status ◽  
Spermatogenic Epithelium ◽  
Chromosome Disjunction

Objective of research: to study the caryopathic effect of bio-preparation Bacillus subtilis 12В on the status of spermatogenic epithelium of white mice after a single intra-abdominal administration of the Fasciola hepatica extract. Materials and methods: The investigations were conducted on white male mice after oral use of preparation Sporovite based on B. subtilis 12B; then the intra-abdominal injection of F. hepatica extracts at a dose of 100 mkg/head was applied. The protein extract from F. hepatica was administered to mice of the second group, and animals of the third group received only the probiotic Sporovite. Animals of the fourth group did not get the preparation and served as controls. 48 hours later the animals were killed; touch smears obtained from testis were stained by the Romanovsky method and examined under a microscope what enables to determine the mitotic index and the number of pathological meiosis forms. The experiments were conducted on lambs whose seminal vesicles were placed into a solution of 10% Formalin and examined histologically. 2-3μ-thick slices were stained with Haematoxylin and Eosin by Van Gieson method and examined under a microscope at 50, 400 and 1000 x magnifications. Results and discussion: During the pathomorphological and caryomitotic studies of testis of white mice and lambs after administration of Sporovite on the background of intra-abdominal injection of F. hepatica extract the reduction of negative effects on the status of spermatogenic epithelium of testis in animals wasn’t observed. A decrease in mitotic index by 2-3 times and a high amount of pathological forms were registered. The number of metaphases with preterm chromosome disjunction under the joint effect of F. hepatica and B. subtilis extracts has decreased by half.

scholarly journals Stage-specific Expression of Dynein Light Chain-1 and Its Interacting Kinase, p21-activated Kinase-1, in Rodent Testes: Implications in Spermiogenesis

2005 ◽  
Vol 53 (10) ◽  
pp. 1235-1243 ◽  
Author(s):  
Rui-An Wang ◽  
Ming Zhao ◽  
Marvin L. Meistrich ◽  
Rakesh Kumar
Keyword(s):  
Light Chain ◽  
Leydig Cells ◽  
Chromatin Condensation ◽  
Dynein Light Chain ◽  
Specific Expression ◽  
Regulatory Interactions ◽  
Dynein Motor ◽  
Complex Process ◽  
P21 Activated Kinase ◽  
Spermatogenic Epithelium

Mammalian spermatogenesis is a complex process involving regulatory interactions of many gene products. In this study, we found that dynein light chain-1 (DLC1), a component of the dynein motor complex, is highly expressed in mouse and rat testes. Immunohistochemically detectable levels of DLC1 are observed specifically in spermatids in steps 9–16 in distinct subcellular compartments: in steps 9–11, DLC1 is predominantly localized in the nucleus; in steps 12 and 13, it is found in both nucleus and cytoplasm; and in step 14–16, it is present exclusively in the cytoplasm. In addition, we found p21-activated kinase 1 (Pak1), a protein kinase that activates DLC1 by phosphorylating DLC1 at Serine 88, was also expressed during these stages of spermatogenesis. Pak1 was also expressed in Leydig cells, in preleptotene primary spermatocytes, and in round spermatids. The spermiogenic stage-specific expression of DLC1 suggests a role for DLC1 in chromatin condensation, spermatid shaping, and the final release of sperm from the spermatogenic epithelium. Further, Pak1 may also play a role in spermiogenesis by regulating DLC1 phosphorylation and, consequently, its function.

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