scholarly journals Role of antizyme in degradation of ornithine decarboxylase in HTC cells

1985 ◽  
Vol 226 (3) ◽  
pp. 893-896 ◽  
Author(s):  
Y Murakami ◽  
S Hayashi
Keyword(s):  
Ornithine Decarboxylase ◽  
Half Life ◽  
Htc Cells

A good correlation was observed between the reciprocal of the half-life of ornithine decarboxylase (ODC) activity in the presence of cycloheximide and the relative amount of ODC-antizyme complex to total ODC (free ODC plus complexed ODC) activity in HTC cells examined at various times after cell dilution or change of medium. Pretreatment of cells with putrescine increased the relative amount of ODC-antizyme complex and decreased the half-life of ODC decay. These results suggested that antizyme plays a key role in ODC degradation.

scholarly journals Ornithine decarboxylase antizyme in kidneys of male and female mice

1988 ◽  
Vol 254 (2) ◽  
pp. 367-372 ◽  
Author(s):  
Y Murakami ◽  
M Marumo ◽  
S I Hayashi
Keyword(s):  
Ornithine Decarboxylase ◽  
Half Life ◽  
Mouse Kidney ◽  
Repeated Injection ◽  
Male Mice ◽  
Protein Inhibitor ◽  
Male And Female ◽  
Female Mice ◽  
Htc Cells

Antizyme, a protein inhibitor of ornithine decarboxylase (ODC), was shown to be induced in mouse kidney by repeated injection of putrescine. Antizyme was also present as a complex with ODC in the kidney of untreated mouse. The amount of the renal ODC-antizyme complex was 3-fold higher in male mice than in female mice. On the contrary, the proportion of ODC present as a complex with antizyme was 24-fold higher in females than in males, and the decay of renal ODC activity after cycloheximide treatment was about 5-fold more rapid in females than in males. Administration of testosterone to female mice, a procedure known to prolong the half-life of renal ODC, increased both ODC activity and the content of ODC-antizyme complex, but decreased the antizyme/ODC ratio in the kidney. These results are consistent with the previous observation in HTC cells that the decay rate of ODC activity in the presence of cycloheximide correlated well with the proportion of ODC present as a complex with antizyme, suggesting the ubiquitous role of antizyme in ODC degradation.

The role of calcium in the induction of ornithine decarboxylase in rat HTC cells

Life Sciences ◽  
1981 ◽  
Vol 29 (7) ◽  
pp. 707-710 ◽  
Author(s):  
Zoe Nakos Canellakis ◽  
Theoharis C. Theoharides ◽  
Philip K. Bondy ◽  
Efthalia T. Triarhos
Keyword(s):  
Ornithine Decarboxylase ◽  
Htc Cells

scholarly journals Half-Life Systematics across theN=126Shell Closure: Role of First-Forbidden Transitions in theβDecay of Heavy Neutron-Rich Nuclei

2014 ◽  
Vol 113 (2) ◽  
Author(s):  
A. I. Morales ◽  
J. Benlliure ◽  
T. Kurtukián-Nieto ◽  
K.-H Schmidt ◽  
S. Verma ◽  
...  
Keyword(s):  
Half Life ◽  
Forbidden Transitions

Prophylaxis for Hemophilia in the Era of Extended Half-Life Factor VIII/Factor IX Products

2016 ◽  
Vol 42 (05) ◽  
pp. 518-525 ◽  
Author(s):  
Erik Berntorp ◽  
Nadine Andersson
Keyword(s):  
Half Life ◽  
Safety Margin ◽  
Clinical Development ◽  
Factor Ix ◽  
Life Extension ◽  
Bleeding Events ◽  
Treatment And Prevention ◽  
Trough Levels ◽  
Life Factor

There are two main bioengineering approaches to extending the half-life of factor (F)VIII or FIX products used for hemophilia replacement therapy. These are fusion to Fc-immunoglobulin G (FVIII and FIX) or to albumin (FIX) or pegylation/glycopegylation (FVIII and FIX). Four FVIII and three FIX products are in clinical development or have recently been licensed in regions of the world. The reported half-life extension is approximately 1.5-fold for FVIII and 2.5-fold, or even longer, for FIX. Clinical trials have shown promising results with respect to extension of dose intervals and efficacy in the treatment and prevention of bleeding events. The role of these products in clinical practice has been discussed in terms of either improving convenience and adherence through prolongation of the interval between infusions or maintaining current intervals thereby increasing trough levels and the safety margin against bleeds. This review of extended half-life products addresses the possibilities and problems of their introduction in hemophilia treatment.

The role of prostaglandin E1 in ornithine decarboxylase induction by tumor promoters

1986 ◽  
Vol 37 (3) ◽  
pp. 445-449 ◽  
Author(s):  
Alfred B. Ordman ◽  
R. C. Simsiman ◽  
Jeffrey S. Cleaveland ◽  
R. K. Boutwell
Keyword(s):  
Ornithine Decarboxylase ◽  
Prostaglandin E1 ◽  
Tumor Promoters

scholarly journals Evaluation of antibiotics as a methodological procedure to inhibit free-living and biofilm bacteria in marine zooplankton culture

2016 ◽  
Vol 88 (suppl 1) ◽  
pp. 733-746 ◽  
Author(s):  
Vanessa O. Agostini ◽  
Alexandre J. Macedo ◽  
Erik Muxagata
Keyword(s):  
Half Life ◽  
Culture Medium ◽  
Metabolic Inhibitors ◽  
Penicillin G ◽  
Streptomycin Sulphate ◽  
Free Living ◽  
Marine Zooplankton ◽  
Scientific Experiments ◽  
Biofilm Bacteria

There is a problem with keeping culture medium completely or partially free from bacteria. The use of prokaryotic metabolic inhibitors, such as antibiotics, is suggested as an alternative solution, although such substances should not harm non-target organisms. Thus, the aim of this study was to assess the effectiveness of antibiotic treatments in inhibiting free-living and biofilm bacteria and their half-life in artificial marine environment using the copepod Acartia tonsa as bioindicador of non-harmful antibiotic combinations. Regarding to results, the application of 0.025 g L-1 penicillin G potassium + 0.08 g L-1 streptomycin sulphate + 0.04 g L-1 neomycin sulphate showed great potential for use in marine cultures and scientific experiments without lethal effects to non-target organisms. The effect of this combination starts within the first six hours of exposure and reduces up to 93 % the bacterial density, but the half-life is short, requiring replacement. No adverse changes in water quality were observed within 168 hours of exposure. As a conclusion, we can infer that this treatment was an effective procedure for zooplankton cultures and scientific experiments with the aim of measuring the role of free-living and biofilm in the marine community.

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