scholarly journals Digitonin perfusion of rat liver. A new approach in the study of intra-acinar and intracellular compartmentation in the liver

1985 ◽  
Vol 226 (1) ◽  
pp. 289-297 ◽  
Author(s):  
B Quistorff ◽  
N Grunnet ◽  
N W Cornell
Keyword(s):  
Rat Liver ◽  
The Other ◽  
Marker Enzymes ◽  
Mitochondrial Marker ◽  
New Approach ◽  
Cytoplasmic Material ◽  
Intracellular Compartmentation ◽  
Intracellular Compartments

Perfusing a rat liver with digitonin in the concentration range 2-20 mg/ml results in complete decolorization of the organ within 45-250 s. Decolorization progresses with time in the direction of flow, and it is therefore possible, by collecting the eluate, to obtain material from specific intracellular compartments of hepatocytes in different zones in the microcirculatory unit of the liver. The results demonstrate that cytoplasmic marker enzymes from periportal or perivenous hepatocytes can be collected with as little contamination from the other compartment as is obtained in micro-dissection studies. Furthermore, a fraction enriched in mitochondrial marker enzymes can be achieved with only 10-20% contamination by cytoplasmic material.

scholarly journals Studies on the glutathione S-transferase activity associated with rat liver mitochondria

1984 ◽  
Vol 222 (2) ◽  
pp. 553-556 ◽  
Author(s):  
C M Ryle ◽  
T J Mantle
Keyword(s):  
Lactate Dehydrogenase ◽  
Rat Liver ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Transferase Activity ◽  
Marker Enzymes ◽  
Glutathione S Transferase ◽  
Mitochondrial Marker ◽  
Liver Glutathione

The major proportion of rat liver glutathione S-transferase is cytosolic. Carefully washed mitochondria contain 0.25-0.47% of the cytosolic activity. Subfractionation of washed mitochondria using digitonin treatment revealed that glutathione S-transferase release did not parallel that of any of the mitochondrial marker enzymes. Glutathione S-transferase release paralleled that of lactate dehydrogenase, suggesting that these ‘mitochondrial’ activities are due to loosely bound cytoplasmic forms.

scholarly journals The subcellular distribution of rat liver serine-pyruvate aminotransferase

1982 ◽  
Vol 202 (2) ◽  
pp. 483-490 ◽  
Author(s):  
K V Rowsell ◽  
L M R Al-Naama ◽  
P Benett
Keyword(s):  
Rat Liver ◽  
Subcellular Distribution ◽  
Subcellular Location ◽  
Mitochondrial Activity ◽  
Transferase Activity ◽  
Mitochondrial Enzymes ◽  
Marker Enzymes ◽  
Aminotransferase Activity ◽  
Mitochondrial Marker ◽  
Differential Sedimentation

1. The subcellular distribution of L-serine-pyruvate aminotransferase activity in rat liver was investigated. About 80% was recovered from cell-free homogenates in a ‘total-particles’ fraction and the remainder in the cytosol. 2. Subfractionation of the particles by differential sedimentation and on sucrose density gradients showed a distribution for serine-pyruvate aminotransferase activity closely matching that observed for mitochondrial marker enzymes. 3. A study of the solubilization of enzymes from combined subcellular particles by digitonin at various concentrations also indicated a common subcellular location for serine-pyruvate aminotransferase and established mitochondrial enzymes. 4. The increase in liver serine-pyruvate amino-transferase activity induced by glucagon injection was accounted for as an increased mitochondrial activity.

A New Approach to the Demonstration of Oxidase-Localization Using Tannic Acid Or Catechin

1973 ◽  
Vol 31 ◽  
pp. 698-699
Author(s):  
V. Mizuhira ◽  
Y. Futaesaku
Keyword(s):  
Cross Section ◽  
Tannic Acid ◽  
Elastic Fiber ◽  
The Other ◽  
New Approach ◽  
Tissue Block ◽  
Active Reaction ◽  
The Cross

Previously we reported that tannic acid is a very effective fixative for proteins including polypeptides. Especially, in the cross section of microtubules, thirteen submits in A-tubule and eleven in B-tubule could be observed very clearly. An elastic fiber could be demonstrated very clearly, as an electron opaque, homogeneous fiber. However, tannic acid did not penetrate into the deep portion of the tissue-block. So we tried Catechin. This shows almost the same chemical natures as that of proteins, as tannic acid. Moreover, we thought that catechin should have two active-reaction sites, one is phenol,and the other is catechole. Catechole site should react with osmium, to make Os- black. Phenol-site should react with peroxidase existing perhydroxide.

MAPPING CONTEMPORARY ISLAMIC JURISPRUDENCE OF MUḤAMMAD SAʻĪD AL-‘ASHMĀWĪ AND MUḤAMMAD SHAḤRŪR

2018 ◽  
Vol 1 (1) ◽  
pp. 32
Author(s):  
Yusroh Yusroh ◽  
Mohd. Zaki Abd. Rahman
Keyword(s):  
The Other ◽  
Russian Philosophy ◽  
New Approach ◽  
Marriage And Divorce ◽  
Other Hand ◽  
Islamic Jurisprudence

Muḥammad Saʻīd Al-‘Ashmāwī and Muḥammad Shaḥrūr are well known as contemporary Muslim thinkers. This article tries to map their contemporary ideas on Islamic jurisprudence. The main data of this research taken mainly from the works both of Al-‘Ashmāwī and Shaḥrūr. In particular, the paper tries to analyze Al-‘Ashmāwī‘s ideas on sharia, politics, hijab, marriage and divorce. On the other hand, the ideas of Shahrour on al-Qur'an, Sunnah and Fiqh, the theory of borders, pluralism, the commandment, inheritance, hijab, marriage, divorce, dowry, politics, and imamate are also critizised. After analyzing their lives and their ideas on Islamic jurisprudence, the paper found that their social, educational and practical backgrounds have affected their intellectual formations and ideas. Ashmawi is encouraged by diligence and enlightenment and is believed to be enlightened. Shahrour, however, takes a new approach in order to create the ḥudūd theory as a new way. As well as their intellectual background, Ashmawi has a good queen in Arabic, English and French as well as religion, Sharia, jurisprudence and theology. Shahrour is a good queen in Arabic, English, Russian, philosophy, philology and historical language.

A NEW APPROACH TO THE STUDY OF COORDINATIVE CONSTRUCTIONS

2019 ◽  
pp. 175-187
Author(s):  
Anna Varnayeva
Keyword(s):  
Characteristic Property ◽  
Mathematical Concept ◽  
The Other ◽  
New Approach ◽  
Logical Aspect ◽  
First Case

Coordinative constructions are traditionally opposed to subordinative constructions. However, this opposition comes down to denial of dependence in coordinative constructions. Thereby the parity of these two constructions does not come to light: subordinative construction can be described without coordinative one. This situation is not improved by detection of a coordinative triangle in all coordinative constructions. The article shows a new approach in the study of coordinative constructions: a coordinative construction is a system; there are not only specific relations – a coordinative triangle, – but also specific elements. Novelty of the study consists in the address to extralinguistic facts, viz. a mathematical concept of a set and its elements. There are a lot of similarities between them. A set in mathematics includes generalizing elements and the composed row in coordinative constructions; in the first case the set is not partitioned, in the second case it is partitioned. In mathematics equivalent components in coordinative constructions correspond to the set elements. A characteristic property in mathematics is homogeneity in coordinative constructions and etc. It is firstly demonstrated, that coordinative and subordinative constructions are correlative and the study of one construction is impossible without the study of the other one. Their parity is shown in coordinative constructions with elements of one set, in subordinative ones with elements of different sets. Cf.: roses and tulips –red roses. In the coordinatiму construction elements of one set are called: «flowers »; in the subordinative construction there are elements of different sets: «flowers » and «colors». It should be noted that the mathematical concept of a set relates to so called logical aspect in linguistics or thinking about reality.

Stress Testing to Assess Recovery from Extreme Events

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
M. John Plodinec
Keyword(s):  
Climate Change ◽  
United States ◽  
Financial Institutions ◽  
Extreme Events ◽  
Stress Testing ◽  
The United States ◽  
The Other ◽  
New Approach ◽  
Rapid Spread ◽  
Potential Benefits

Abstract Over the last decade, communities have become increasingly aware of the risks they face. They are threatened by natural disasters, which may be exacerbated by climate change and the movement of land masses. Growing globalization has made a pandemic due to the rapid spread of highly infectious diseases ever more likely. Societal discord breeds its own threats, not the least of which is the spread of radical ideologies giving rise to terrorism. The accelerating rate of technological change has bred its own social and economic risks. This widening spectrum of risk poses a difficult question to every community – how resilient will the community be to the extreme events it faces. In this paper, we present a new approach to answering that question. It is based on the stress testing of financial institutions required by regulators in the United States and elsewhere. It generalizes stress testing by expanding the concept of “capital” beyond finance to include the other “capitals” (e.g., human, social) possessed by a community. Through use of this approach, communities can determine which investments of its capitals are most likely to improve its resilience. We provide an example of using the approach, and discuss its potential benefits.

Closed Sets of Boolean Terms in Relational Databases

1991 ◽  
Vol 14 (3) ◽  
pp. 367-385
Author(s):  
Andrzej Jankowski ◽  
Zbigniew Michalewicz
Keyword(s):  
Computational Complexity ◽  
Incomplete Information ◽  
Relational Databases ◽  
The Other ◽  
New Approach ◽  
Closed Sets ◽  
Null Value ◽  
Algebraic Operations

A number of approaches have been taken to represent compound, structured values in relational databases. We review a few such approaches and discuss a new approach, in which every set is represented as a Boolean term. We show that this approach generalizes the other approaches, leading to more flexible representation. Boolean term representation seems to be appropriate in handling incomplete information: this approach generalizes some other approaches (e.g. null value mark, null variables, etc). We consider definitions of algebraic operations on such sets, like join, union, selection, etc. Moreover, we introduce a measure of computational complexity of these operations.

scholarly journals Exploring the Effect of Silicon on the High Temperature Corrosion of Lean FeCrAl Alloys in Humid Air

2021 ◽  
Author(s):  
T. Sand ◽  
A. Edgren ◽  
C. Geers ◽  
V. Asokan ◽  
J. Eklund ◽  
...  
Keyword(s):  
Corrosion Resistance ◽  
High Temperature ◽  
Oxidation Behavior ◽  
High Temperature Corrosion ◽  
The Other ◽  
Oxide Scales ◽  
Protective Oxide ◽  
New Approach ◽  
Fecral Alloys ◽  
Cr Evaporation

AbstractA new approach to reduce the chromium and aluminium concentrations in FeCrAl alloys without significantly impairing corrosion resistance is to alloy with 1–2 wt.% silicon. This paper investigates the “silicon effect” on oxidation by comparing the oxidation behavior and scale microstructure of two FeCrAl alloys, one alloyed with silicon and the other not, in dry and wet air at 600 °C and 800 °C. Both alloys formed thin protective oxide scales and the Cr-evaporation rates were small. In wet air at 800 °C the Si-alloyed FeCrAl formed an oxide scale containing mullite and tridymite together with α- and γ-alumina. It is suggested that the reported improvement of the corrosion resistance of Al- and Cr-lean FeCrAl’s by silicon alloying is caused by the appearance of Si-rich phases in the scale.

scholarly journals The fractionation of nuclei from mammalian cells by zonal centrifugation

1968 ◽  
Vol 109 (1) ◽  
pp. 127-135 ◽  
Author(s):  
I R Johnston ◽  
A P Mathias ◽  
F. Pennington ◽  
D. Ridge
Keyword(s):  
Rat Liver ◽  
Mammalian Cells ◽  
Mouse Liver ◽  
The Other ◽  
Slow Speed ◽  
Adult Rats ◽  
Sucrose Solutions ◽  
Liver Nuclei ◽  
Effect Of Age ◽  
Zonal Rotor

1. Purified liver nuclei from adult rats separate into two main zones when centrifuged in the slow-speed zonal rotor. One zone contains diploid nuclei, the other tetraploid. 2. The effect of age on the pattern of rat liver ploidy was examined. Tetraploid nuclei are virtually absent from young animals. They increase in proportion steadily with age. Partial hepatectomy disturbs the pattern of ploidy. 3. The zonal centrifuge permits the separation of diploid, tetraploid, octaploid and hexadecaploid nuclei from mouse liver. 4. Rat liver nuclei are isopycnic with sucrose solutions of density 1·35 at 5°.

Analytical Subcellular Fractionation Studies on Rat Liver and on Isolated Jejunal Enterocytes with Special Reference to the Separation of Lysosomes, Peroxisomes and Mitochondria

1976 ◽  
Vol 50 (5) ◽  
pp. 355-366 ◽  
Author(s):  
T. J. Peters ◽  
H. Shio
Keyword(s):  
Rat Liver ◽  
Sucrose Density Gradient ◽  
Subcellular Fractionation ◽  
Low Molecular Weight ◽  
Rat Jejunum ◽  
Marker Enzymes ◽  
Good Separation ◽  
Subcellular Organelles ◽  
Rat Liver Cells ◽  
Isopycnic Centrifugation

1. Enterocytes were isolated from rat jejunum and characterized morphologically. 2. Attempts to separate the enterocyte subcellular organelles, characterized by their marker enzymes, with isopycnic centrifugation were unsuccessful but good separation of peroxisomes, lysosomes and mitochondria was achieved by sedimentation through a shallow sucrose density gradient with a superimposed inverse gradient of low-molecular-weight dextran. 3. The properties and enzyme activities of the principal subcellular organelles in rat liver cells and enterocytes were compared.

Sign in / Sign up

Export Citation Format

Share Document