scholarly journals Localization of Tamm-Horsfall-glycoprotein-like immunoreactivity in cultured baby-hamster kidney cells, shown by immunofluorescence and by light- and electron-microscopic immunoperoxidase techniques

1985 ◽  
Vol 225 (2) ◽  
pp. 481-486 ◽  
Author(s):  
K L Sikri ◽  
C L Foster ◽  
R D Marshall
Keyword(s):  
Plasma Membranes ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Microscopic Technique ◽  
Electron Microscopic ◽  
The Third ◽  
Epithelial Origin ◽  
Renal Tubular ◽  
Hamster Urine ◽  
Baby Hamster Kidney Cells

Tamm-Horsfall glycoprotein was isolated from hamster urine, and antiserum against it was produced in rabbits. IgG was isolated from the antiserum. Immunocytochemical methods were used to localize Tamm-Horsfall-like immunoreactivity in three substrains of baby-hamster kidney (BHK) cells. Indirect immunofluorescence techniques showed that, in two substrains (BHK-21/C13/2P and BHK-21/C13/3P), a proportion of the cells fluoresced brilliantly, whereas those of the third substrain (BHK-21/ICRF) were totally negative. Related findings were obtained by the immunoperoxidase optical-microscopic technique. From the results of immunoperoxidase techniques using the electron microscope, it was concluded that the substance was present in association with the plasma membranes of the reacting cells. Our data suggest that the line of baby-hamster kidney cells, BHK-21/C13, may contain cells of renal-tubular epithelial origin, and that the proportion of these may be variable from one subculture to another.

scholarly journals Immunocytochemical study of the partition and distribution of Sindbis virus glycoproteins in freeze-fractured membranes of infected baby hamster kidney cells.

1985 ◽  
Vol 101 (4) ◽  
pp. 1300-1306 ◽  
Author(s):  
M R Torrisi ◽  
S Bonatti
Keyword(s):  
Plasma Membrane ◽  
Sindbis Virus ◽  
Plasma Membranes ◽  
Protein A ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Immunocytochemical Study ◽  
Viral Glycoproteins ◽  
Infected Cells ◽  
Baby Hamster Kidney Cells

Sindbis virus-infected baby hamster kidney cells were analyzed by thin section fracture-label. Specific immunolabel with antiviral glycoprotein antibodies or with conventional lectin label (wheat germ agglutinin) were used in conjunction with colloidal gold-conjugated protein A or ovomucoid, respectively. In addition, intact infected cells were analyzed with both labeling procedures. Experiments with Sindbis infected-chick embryo fibroblast cells were carried out as controls. Viral transmembrane glycoproteins appeared present in freeze-fractured inner and outer nuclear membrane, endoplasmic reticulum, Golgi stacks and vesicles, and plasma membranes; a clear preferential partition with the exoplasmic faces of all intracellular membranes was observed. By contrast, at the plasma membrane level, Sindbis glycoproteins were found to partition preferentially with the protoplasmic face. It seems likely that this protoplasmic partition is related to the binding with the nucleocapsid that takes place during the budding of the virus. At the cell surface, viral glycoproteins always appeared clustered and were predominantly associated with budding figures: moreover, large portions of the plasma membrane were devoid of both glycoproteins and budding viruses.

Turbomolecular Pumped Electron Microscopy of Rubella Virus (In BHK Celis)

1968 ◽  
Vol 26 ◽  
pp. 204-205
Author(s):  
A. B. Taylor ◽  
G. C. Cole ◽  
M. A. Holcomb ◽  
C. A. Baechler
Keyword(s):  
Electron Microscopy ◽  
Rubella Virus ◽  
Phosphate Buffer ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Basal Medium ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Input Multiplicity ◽  
Baby Hamster Kidney Cells

An aliquot from a continuous fermenter culture of baby hamster kidney cells (BHK-21 Clone PD-4) (Wistar) maintained in Ca free Eagle's Basal Medium containing 2% Kaolin adsorbed fetal calf serum was planted in spinner flasks at 300,000 cells per ml, total volume 600 ml. After equilibration for one day at 35°C to insure that cells were in log phase, the culture was infected with the M-33-AGMK25 BHK-219 strain of rubella at an input multiplicity of about 6 TCID50 per cell. The virus was identified with specific rubella antiserum.Preliminary experiments had shown that such cultures would reach a peak or plateau HA titer of approximately 1:64, 24 hrs after inoculation and would continue to yield virus for 6 to 12 days. One hundred ml aliquot harvests were withdrawn daily and the culture was returned to volume with growth medium and incubation continued. The harvested cells were spun down rapidly at 2500 rpm per 15 mins., fixed in 3.7% gluteraldehyde in Ca free phosphate buffer saline, and post fixed in osmium tetraoxide. After dehydration, the cells were embedded in Epon 812 and cured approximately 20 hrs at 60°C.

scholarly journals Allicin from garlic strongly inhibits cysteine proteinases and cytopathic effects of Entamoeba histolytica.

1997 ◽  
Vol 41 (10) ◽  
pp. 2286-2288 ◽  
Author(s):  
S Ankri ◽  
T Miron ◽  
A Rabinkov ◽  
M Wilchek ◽  
D Mirelman
Keyword(s):  
Alcohol Dehydrogenase ◽  
Entamoeba Histolytica ◽  
Kidney Cells ◽  
Cysteine Proteinases ◽  
Baby Hamster Kidney ◽  
Important Contributor ◽  
Cytopathic Effects ◽  
Baby Hamster Kidney Cells

The ability of Entamoeba histolytica trophozoites to destroy monolayers of baby hamster kidney cells is inhibited by allicin, one of the active principles of garlic. Cysteine proteinases, an important contributor to amebic virulence, as well as alcohol dehydrogenase, are strongly inhibited by allicin.

scholarly journals Cytotoxicity of Pristine C<sub>60</sub> Fullerene on Baby Hamster Kidney Cells in Solution

2012 ◽  
Vol 03 (03) ◽  
pp. 385-390 ◽  
Author(s):  
Shufang Liu ◽  
Haijie Liu ◽  
Zhijuan Yin ◽  
Kai Guo ◽  
Xibao Gao
Keyword(s):  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Baby Hamster Kidney Cells

scholarly journals Uptake and utilization of 5'-methylthioadenosine by cultured baby-hamster kidney cells

1982 ◽  
Vol 204 (3) ◽  
pp. 803-807 ◽  
Author(s):  
T O Eloranta ◽  
K Tuomi ◽  
A M Raina
Keyword(s):  
Nucleic Acids ◽  
Cellular Metabolism ◽  
Exponential Phase ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Purine Nucleotide ◽  
Salvage Pathway ◽  
Baby Hamster Kidney Cells

5'-Methylthioadenosine was taken up and immediately metabolized further by cultured baby-hamster kidney cells during the exponential phase of growth. The adenine moiety supplied the purine-nucleotide pool via the salvage pathway and was efficiently incorporated into nucleic acids. Catabolites of methylthioadenosine excreted by the cells included adenine, purinic compounds and metabolites of the ribose portion. 5'-Methylthiotubercidin had no significant effect on the cellular metabolism of methyl-thioadenosine, but greatly inhibited its uptake. erythro-9-(2-Hydroxy-3-nonyl)adenine had no effect on the uptake, but markedly interfered with the further utilization of methylthioadenosine after cleavage in the cells.

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