scholarly journals Determination of tryptophan oxygenase activity in rat liver homogenates

1984 ◽  
Vol 221 (2) ◽  
pp. 557-558 ◽  
Author(s):  
L Stowell ◽  
J Mørland
Keyword(s):  
Rat Liver ◽  
Tryptophan Oxygenase ◽  
Oxygenase Activity ◽  
Liver Homogenates

scholarly journals The influence of some methodological factors on measurement of tryptophan oxygenase activities in crude homogenates of rat liver

1983 ◽  
Vol 209 (3) ◽  
pp. 831-836 ◽  
Author(s):  
L Stowell ◽  
J Mørland
Keyword(s):  
Rat Liver ◽  
Liver Tissue ◽  
Post Mortem ◽  
Temperature Dependent ◽  
Tryptophan Oxygenase ◽  
Oxygenase Activity ◽  
Liver Homogenates ◽  
Independent Reaction

1. With two different methods for assaying the tryptophan oxygenase activity in rat liver homogenates, the effects of some methodological factors on the activity of the enzyme were studied. 2. In fed, but not in starved, rats a compound(s) absorbing at 365 nm, interfering with the reading of kynurenine absorbance, disappeared gradually during incubation. 3. A correction for this tryptophan-independent reaction was necessary in order to determine correct tryptophan oxygenase activity. 4. Blood remaining in liver tissue post mortem can serve as a source of cofactor haem for tryptophan oxygenase, causing spuriously high values for the activity of the holoenzyme form of tryptophan oxygenase. 5. A rapid and progressive activation of tryptophan oxygenase post mortem occurs in undisrupted liver tissue, and this activation is temperature-dependent.

scholarly journals Determination of tryptophan pyrrolase activity in rat liver homogenates

1983 ◽  
Vol 215 (3) ◽  
pp. 709-710 ◽  
Author(s):  
A A B Badawy ◽  
C J Morgan ◽  
N R Davis
Keyword(s):  
Rat Liver ◽  
Tryptophan Pyrrolase ◽  
Liver Homogenates

scholarly journals Determination of bilirubin glucuronide and assay of glucuronyltransferase with bilirubin as acceptor

1968 ◽  
Vol 107 (4) ◽  
pp. 507-518 ◽  
Author(s):  
F. P. Van Roy ◽  
K. P. M. Heirwegh
Keyword(s):  
Rat Liver ◽  
Chromatographic Analysis ◽  
Diazonium Salt ◽  
Enzyme Systems ◽  
Liver Homogenates ◽  
Conjugated Bilirubin

1. Conjugated bilirubin is conveniently determined by coupling with the diazonium salt of ethyl anthranilate. 2. This method has been used in the development of assays for UDP-glucuronyltransferase (EC 2.4.1.17), with bilirubin as substrate, in rat liver homogenates, microsomal preparations and partly purified fractions. 3. Chromatographic analysis suggests that bilirubin monoglucuronide is the product of the enzyme systems studied.

Determination of trioses in tungstate supernatants of rat liver homogenates

1972 ◽  
Vol 46 (1) ◽  
pp. 352-355 ◽  
Author(s):  
D.J. Walton ◽  
Lauralyn M. Gauchie
Keyword(s):  
Rat Liver ◽  
Liver Homogenates

scholarly journals ON THE DETERMINATION OF LIVER XANTHINE OXIDASE AND THE RESPIRATION OF RAT LIVER HOMOGENATES

1949 ◽  
Vol 181 (1) ◽  
pp. 255-271 ◽  
Author(s):  
Dan A. Richert ◽  
Sally. Edwards ◽  
W.W. Westerfeld
Keyword(s):  
Xanthine Oxidase ◽  
Rat Liver ◽  
Liver Homogenates
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