scholarly journals Isolation and identification of ecdysteroid phosphates and acetylecdysteroid phosphates from developing eggs of the locust, Schistocerca gregaria

1984 ◽  
Vol 221 (2) ◽  
pp. 459-464 ◽  
Author(s):  
R E Isaac ◽  
H H Rees
Keyword(s):  
High Performance ◽  
Fast Atom Bombardment ◽  
Schistocerca Gregaria ◽  
Phosphate Esters ◽  
Enzymic Hydrolysis ◽  
Isolation And Identification ◽  
Insect Eggs ◽  
Polar Extracts ◽  
Moulting Hormone ◽  
Acetyl Migration

Maturing eggs of the desert locust, Schistocerca gregaria, contain a variety of ecdysteroid (insect moulting hormone) conjugates and metabolites, four of which have been previously isolated from polar extracts and identified as ecdysonoic acid, 20-hydroxyecdysonoic acid, 3-acetylecdysone 2-phosphate and ecdysone 2-phosphate. In the present study we have isolated eight additional ecdysteroids from similar late-stage eggs by high-performance liquid chromatography. The 22-phosphate esters of ecdysone, 2-deoxyecdysone, 20-hydroxyecdysone and 2-deoxy-20-hydroxyecdysone, all of which were first identified as ecdysteroid components of newly-laid eggs of S. gregaria, were identified by co-chromatography with authentic compounds and by physicochemical techniques. The remaining compounds were identified as 3-acetyl-20-hydroxyecdysone 2-phosphate, 3-epi-2-deoxyecdysone 3-phosphate, 3-acetylecdysone 22-phosphate and 2-acetylecdysone 22-phosphate by fast atom bombardment mass spectrometry, p.m.r. spectroscopy and analysis of the steroid moieties after enzymic hydrolysis. The latter two compounds, after isolation, are susceptible to nonenzymic acetyl migration and deacetylation to give mixtures of ecdysone 22-phosphate and its 2- and 3-acetate derivatives. The possible role and significance of these ecdysteroid conjugates with respect to the control of hormone titres in insect eggs is discussed.

scholarly journals Identification of the 22-phosphate esters of ecdysone, 2-deoxyecdysone, 20-hydroxyecdysone and 2-deoxy-20-hydroxyecdysone from newly laid eggs of the desert locust, Schistocerca gregaria

1983 ◽  
Vol 213 (2) ◽  
pp. 533-541 ◽  
Author(s):  
R E Isaac ◽  
M E Rose ◽  
H H Rees ◽  
T W Goodwin
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Fast Atom Bombardment ◽  
Schistocerca Gregaria ◽  
Reversed Phase ◽  
Phosphate Esters ◽  
Desert Locust ◽  
Phosphate Analysis ◽  
Moulting Hormone

The four major ecdysteroid (insect moulting hormone) conjugates present in the newly laid eggs of the desert locust, Schistocera gregaria, have been purified by reversed-phase and anion-exchange high-performance liquid chromatography. The steroid moieties were identified as ecdysone, 2-deoxyecdysone, 20-hydroxyecdysone and 2-deoxy-20-hydroxyecdysone. Phosphate analysis of acid-hydrolysed samples showed a steroid:phosphate ratio of approx. 1:1 for all four compounds. The intact conjugates were identified as ecdysone 22-phosphate, 2-deoxyecdysone 22-phosphate, 20-hydroxyecdysone 22-phosphate and 2-deoxy-20-hydroxyecdysone 22-phosphate by fast atom bombardment mass spectrometry and 1H, 13C and 31P n.m.r. The significance of ecdysteroid phosphates as a source of free hormone during embryogenesis is discussed.

scholarly journals Identification of ecdysonoic acid and 20-hydroxyecdysonoic acid isolated from developing eggs of Schistocerca gregaria and pupae of Spodoptera littoralis

1983 ◽  
Vol 213 (1) ◽  
pp. 261-265 ◽  
Author(s):  
R E Isaac ◽  
N P Milner ◽  
H H Rees
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Methyl Ester ◽  
Spodoptera Littoralis ◽  
High Performance ◽  
Fast Atom Bombardment ◽  
Schistocerca Gregaria ◽  
Methyl Esters ◽  
Desert Locust

Ecdysonoic acid and 20-hydroxyecdysonoic acid have been purified from developing eggs of the desert locust, Schistocerca gregaria, by high performance liquid chromatography (h.p.l.c.), and their structures were determined by p.m.r. spectroscopy and fast atom bombardment mass spectrometry of the free and methyl ester derivatives. 20-Hydroxyecdysonoic acid was also characterized from Spodoptera littoralis pupae. The occurrence of both 20-hydroxyecdysonoic acid and ecdysonoic acid in Sp. littoralis pupae was also established by h.p.l.c. comparison of the 3H-labelled acids formed from [3H]ecdysone and of their methyl esters with the corresponding substances from Sch. gregaria. The significance of ecdysteroid acids as products of ecdysteroid inactivation is discussed.

scholarly journals Isolation and identification of 3-acetylecdysone 2-phosphate, a metabolite of ecdysone, from developing eggs of Schistocerca gregaria

1984 ◽  
Vol 217 (1) ◽  
pp. 239-243 ◽  
Author(s):  
R E Isaac ◽  
H P Desmond ◽  
H H Rees
Keyword(s):  
High Performance ◽  
Schistocerca Gregaria ◽  
Helix Pomatia ◽  
Reversed Phase ◽  
Ionization Mass ◽  
Isolation And Identification ◽  
Ecdysteroid Conjugate ◽  
Hydrolysis Of

A major ecdysteroid conjugate, which accumulates in the eggs of the desert locust, Schistocerca gregaria, during the later stages of embryogenesis, has been isolated by reversed-phase and anion-exchange high-performance liquid chromatography. Hydrolysis of the conjugate with a crude arylsulphatase preparation from Helix pomatia liberates mainly ecdysone 3-acetate. The compound was identified as 3-acetylecdysone 2-phosphate by phosphate analysis of an acid-hydrolysed sample, fast atom bombardment, electron impact and chemical ionization mass spectrometry and 1H and 13Cn.m.r. spectroscopy. The instability of 3-acetylecdysone 2-phosphate on storage results in the formation of ecdysone 2-phosphate, which was identified by physicochemical techniques. 3-Acetylecdysone 2-phosphate and ecdysone 2-phosphate are less susceptible than ecdysone 22-phosphate to hydrolysis in vitro by an enzyme preparation from S. gregaria embryos. The possible role of 3-acetylecdysone 2-phosphate as an inactive end product of ecdysteroid metabolism is discussed.

Phytoceuticals in aniseeds: a practical approach for isolation and identification

2016 ◽  
Vol 5 (01) ◽  
pp. 4701
Author(s):  
Shobha R. I. ◽  
Andallu B.
Keyword(s):  
Chronic Diseases ◽  
Column Chromatography ◽  
High Performance ◽  
Methanolic Extract ◽  
Analytical Techniques ◽  
Therapeutic Agents ◽  
Systematic Research ◽  
Isolation And Identification ◽  
Traditional System ◽  
Pharmacologically Active

Nature has provided mankind with a broad and structurally diverse array of pharmacologically active chemical compounds, phytoceuticals, which have proved to be indispensable for the cure of chronic diseases or as lead structures for novel therapeutic agents. Almost, 70% of modern medicines in India have been developed from plants used in the traditional system of medicine. With the technological advances and the development of more sophisticated isolation and analytical techniques, there is great scope for further systematic research to screen and isolate many more phytoceuticals which might be more effective/as effective as synthetic drugs and thereby assess their potential in protecting against chronic diseases. The present investigation is aimed at isolation and identification of phytoceuticals in a spice, aniseed (Pimpinella anisum L), therapeutically less-exploited and widely used only for culinary purpose, using analytical techniques viz. column chromatography, high performance thin layer chromatography (HPTLC.) and nuclear magnetic resonance spectrometry. Among all the solvent fractions of methanolic extract tested, methanolic extract and ethyl acetate fraction possessed highest amounts of bioactive compounds viz. phenolics, flavonoids, flavonols which was confirmed by qualitative, quantitative and HPTLC. analyses. Spectral analysis using NMR. of one of the sub-fractions of aniseeds obtained by column chromatography, revealed the presence of a glycosylated flavone, luteolin-6C-glucoside in aniseeds.  The present study revealed aniseeds to be a great source of bioactive phytoceuticals which can be novel candidates for development of new therapeutic agents.

Isolation and identification of tuliposides D and F from tulip cultivars

2020 ◽  
Vol 75 (1-2) ◽  
pp. 7-12 ◽  
Author(s):  
Taiji Nomura ◽  
Shinjiro Ogita ◽  
Yasuo Kato
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Secondary Metabolites ◽  
High Performance ◽  
The Other ◽  
Isolation And Identification ◽  
Tulipa Gesneriana ◽  
Tissue Extracts ◽  
Spectroscopic Analyses ◽  
Hplc Peak

Abstract6-Tuliposides A (6-PosA) and B (6-PosB) are major defensive secondary metabolites in tulip cultivars (Tulipa gesneriana), having an acyl group at the C-6 position of d-glucose. Although some wild tulip species produce 1,6-diacyl-glucose type of Pos (PosD and PosF), as well as 6-PosA/B, they have not yet been isolated from tulip cultivars. Here, aiming at verifying the presence of PosD and PosF in tulip cultivars, tissue extracts of 25 cultivars were analyzed by high-performance liquid chromatography (HPLC). Although no HPLC peaks for PosD nor PosF were detected in most cultivars, we found two cultivars giving a minute HPLC peak for PosD and the other two cultivars giving that for PosF. PosD and PosF were then purified from petals of cultivar ‘Orca’ and from pistils of cultivar ‘Murasakizuisho’, respectively, and their identities were verified by spectroscopic analyses. This is the first report that substantiates the presence of 1,6-diacyl-glucose type of Pos in tulip cultivars.

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