scholarly journals The kinetics of ammonia release during the catalytic cycle of pig plasma amine oxidase

1984 ◽  
Vol 220 (3) ◽  
pp. 767-772 ◽  
Author(s):  
F X Rius ◽  
P F Knowles ◽  
G Pettersson
Keyword(s):  
Time Course ◽  
Amine Oxidase ◽  
Catalytic Cycle ◽  
Sensitive Assay ◽  
Ammonia Release ◽  
Plasma Amine Oxidase ◽  
Kinetics Of ◽  
Enzyme Species

The time course of NH3 release during the catalytic cycle of pig plasma amine oxidase was followed by using the quenched-flow technique in conjunction with a sensitive assay for NH3. These studies were made under both air and O2-saturating conditions. The results establish unequivocally that NH3 is released in the step whereby a reduced enzyme species is re-oxidized by molecular O2 rather than in the step leading to the reduced enzyme. It is concluded that the catalytic cycle of the enzyme conforms to an aminotransferase mechanism rather than one in which an imine is an intermediate.

Influence of Radioiodine Therapy on Urinary Iodine Excretion

1998 ◽  
Vol 37 (03) ◽  
pp. 107-112 ◽  
Author(s):  
I. Lauer ◽  
M. Bähre ◽  
E. Richter ◽  
B. Melier
Keyword(s):  
Time Course ◽  
Radioiodine Therapy ◽  
Urinary Iodine ◽  
Target Volume ◽  
Urinary Iodine Excretion ◽  
Urinary Creatinine ◽  
Iodine Excretion ◽  
Kinetics Of ◽  
Persistent Elevation ◽  
Benign Thyroid

Summary Aim: In 214 patients with benign thyroid diseases the time-course of urinary iodine excretion (UIE) was investigated in order to identify changes after radioiodine therapy (RITh). Method: UIE was measured photometrically (cerium-arsenite method) and related to urinary creatinine on the first and last day of the radioiodine test and then three days, seven days, four weeks, and six months after 1311 administration. Results: As compared with the level found immediately before radioiodine therapy, median UIE had almost doubled four weeks after therapy and was still significantly elevated six months after therapy. This increase correlated significantly with the target volume as measured by scintigraphy and sonography. Conclusions: The persistent elevation of UIE for months after RITh is a measure of treatment-induced damage to thyrocytes. Therefore, in view of the unfavourable kinetics of iodine that follow it, RITh should if possible be given via a single-dose regime.

Amine oxidase. XII. Association and dissociation, and number of subunits of beef plasma amine oxidase

Biochemistry ◽  
1968 ◽  
Vol 7 (12) ◽  
pp. 4329-4335 ◽  
Author(s):  
Frances M. Achee ◽  
Charles H. Chervenka ◽  
Richard A. Smith ◽  
Kerry T. Yasunobu
Keyword(s):  
Amine Oxidase ◽  
Plasma Amine Oxidase

scholarly journals Changes in J chain and mu chain RNA expression as a function of B cell differentiation.

1984 ◽  
Vol 160 (3) ◽  
pp. 877-892 ◽  
Author(s):  
G Lamson ◽  
M E Koshland
Keyword(s):  
B Cell ◽  
Time Course ◽  
Rna Synthesis ◽  
Rna Expression ◽  
B Cell Differentiation ◽  
Activated Lymphocytes ◽  
J Chain ◽  
Pattern Characteristic ◽  
Kinetics Of ◽  
Lymphoid Cell Lines

The time course of differentiative events in the pentamer IgM response was examined by following the expression of J chain and mu chain RNA and their protein products in mitogen-stimulated lymphocytes. The analyses showed that the shift to mus RNA synthesis begins shortly after stimulation and precedes proliferation of the cells and any increase in mu RNA levels. In contrast, expression of J chain RNA and the amplification of J chain and mus message are late events that coincide with a phase of rapid proliferation and with the secretion of pentamer IgM antibody. The kinetics of J and mu chain RNA expression observed in normal lymphocytes were supported by analyses of lymphoid cell lines. B lymphomas were found to display the RNA pattern characteristic of early-activated lymphocytes, i.e., a partial shift to mus RNA production and no J chain RNA, whereas IgM-secreting lines resembled late-activated lymphocytes in their expression of high levels of both mus and J chain mRNA. Moreover, the kinetics of J and mus chain RNA expression correlates with the sequential action of B cell lymphokines in the induction of the pentamer IgM response. This correlation suggests that the successive differentiative changes are triggered by successive membrane stimuli.

Purification and active-site characterization of equine plasma amine oxidase

1994 ◽  
Vol 56 (2) ◽  
pp. 127-141 ◽  
Author(s):  
Scott R. Carter ◽  
Michele A. McGuirl ◽  
Doreen E. Brown ◽  
David M. Dooley
Keyword(s):  
Active Site ◽  
Amine Oxidase ◽  
Site Characterization ◽  
Equine Plasma ◽  
Plasma Amine Oxidase

Novel inactivators of plasma amine oxidase

1975 ◽  
Vol 97 (19) ◽  
pp. 5613-5614 ◽  
Author(s):  
Alan L. Maycock ◽  
Robert H. Suva ◽  
Robert H. Abeles
Keyword(s):  
Amine Oxidase ◽  
Plasma Amine Oxidase

Biogenic amine-related enzymes and personality variations in normals

1977 ◽  
Vol 7 (1) ◽  
pp. 149-157 ◽  
Author(s):  
D. L. Murphy ◽  
R. H. Belmaker ◽  
M. Buchsbaum ◽  
N. F. Martin ◽  
R. Ciaranello ◽  
...  
Keyword(s):  
Biogenic Amine ◽  
Sensation Seeking ◽  
Amine Oxidase ◽  
Psychiatric Patients ◽  
Chronic Schizophrenia ◽  
Personality Variables ◽  
Scale Scores ◽  
Plasma Amine Oxidase ◽  
Positive Correlations ◽  
Sensation Seeking Scale

SynopsisAltered activities of biogenic amine-related enzymes have been reported in some studies of patients with affective disorders and chronic schizophrenia. To evaluate whether any relationship between personality variations, including non-diagnosed psychopathology, and these enzymes might occur in non-psychiatric patients, 95 young adult volunteers were studied. Higher MMPI and Zuckerman sensation-seeking scale scores were found in male, but not female, volunteers with lower platelet and plasma amine oxidase activities. Males generally exhibited negative correlations, while females had positive correlations between the psychological test scores and amine oxidase activities. In contrast to the amine oxidase results, no associations were observed between plasma dopamine β-hydroxylase activities and the personality variables.

Coordinate induction of metallothioneins I and II in rodent cells by UV irradiation

1988 ◽  
Vol 8 (11) ◽  
pp. 4716-4720
Author(s):  
A J Fornace ◽  
H Schalch ◽  
I Alamo
Keyword(s):  
Sequence Analysis ◽  
Uv Irradiation ◽  
Time Course ◽  
Human Fibroblasts ◽  
Chinese Hamster ◽  
Cdna Clones ◽  
X Rays ◽  
General Stress Response ◽  
Rapid Induction ◽  
Kinetics Of

Sequence analysis of Chinese hamster V79 lung fibroblast cDNA clones, which code for UV radiation-inducible transcripts, revealed that many of the clones corresponded to metallothioneins (MTs) I and II. A third cDNA clone, DDIU4, was found also to code for a similar-size UV-inducible transcript which was unrelated to MT by both sequence analysis and kinetics of induction. MTI and MTII RNAs rapidly increased in V79 cells within 1 h after UV irradiation, and maximum induction was seen by 4 h. This rapid induction of MT RNA by UV irradiation was not observed in human fibroblasts. MTI and MTII were coordinately induced in both time course and dose-response experiments, although the induction of MTII, up to 30-fold, was three to four times greater than that of MTI. The induction of MT did not appear to be a general stress response, since no increase occurred after exposure to X rays or H2O2.

scholarly journals Electron nuclear double-resonance (ENDOR) spectroscopy of amine oxidase from pig plasma

1986 ◽  
Vol 237 (2) ◽  
pp. 609-612 ◽  
Author(s):  
G J Baker ◽  
P F Knowles ◽  
K B Pandeya ◽  
J B Rayner
Keyword(s):  
Hyperfine Coupling ◽  
Amine Oxidase ◽  
Double Resonance ◽  
Spectroscopic Studies ◽  
Water Molecules ◽  
Electron Nuclear Double Resonance ◽  
Endor Spectroscopy ◽  
Amino Acid Histidine ◽  
Plasma Amine Oxidase ◽  
Hyperfine Couplings

Electron nuclear double-resonance (‘ENDOR’) spectroscopic studies on pig plasma amine oxidase have been carried out at 15 K. Deuterium-exchange studies show the presence of two sets of exchangeable protons, probably from two water molecules; from the magnitude of their hyperfine couplings, one is assigned to be equatorially, and the other axially, co-ordinated. Only one 14N hyperfine coupling is observed, suggesting that the bonding of all amino acid (histidine) or organic cofactor ligands is similar. Upon addition of azide, a further hyperfine coupling to nitrogen is observed which is smaller than that observed for the native enzyme; the hyperfine couplings to the remaining nitrogens are slightly altered.

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