scholarly journals Adsorbed soluble [3H]elastin as substrate for proteinase activity. A new microassay technique

1984 ◽  
Vol 218 (2) ◽  
pp. 645-648 ◽  
Author(s):  
H S Jensen
Keyword(s):  
Coefficient Of Variation ◽  
Proteinase Activity ◽  
Elastase Activity ◽  
Pancreatic Elastase ◽  
Passive Adsorption ◽  
Interassay Coefficient ◽  
Scintillation Fluid ◽  
Porcine Pancreatic Elastase

A microassay for elastase activity has been designed. Microtubes are coated with soluble elastin substrate by passive adsorption to the tube walls. On contact with enzymes in test samples, radioactivity is released into the sample buffer, which is then transferred to vials containing scintillation fluid without further processing. Porcine pancreatic elastase (EC 3.4.21.11) is easily detected in concentrations down to 8 micrograms/litre. The interassay coefficient of variation (CV) was below 10% for values above 50 micrograms/litre. The assay is rapid, precise, economical and sensitive.

scholarly journals Studies on porcine pancreatic elastase activity. I. Radioimmuoassay of porcine pancreatic elastase.

1980 ◽  
Vol 131 (2) ◽  
pp. 119-126 ◽  
Author(s):  
YASUYUKI NAKAJIMA ◽  
SEIKI MATSUNO ◽  
NOBORU NOTO ◽  
YOICHI SAITOR ◽  
TOSHIO SATO
Keyword(s):  
Elastase Activity ◽  
Pancreatic Elastase ◽  
Porcine Pancreatic Elastase

The Effects of Various Lipids upon the Rate of Elastolysis of Bovine Ligamentum Nuchae Elastin by Porcine Pancreatic Elastase

1985 ◽  
Vol 68 (s11) ◽  
pp. 26P-27P
Author(s):  
S A Wharton ◽  
G J Phillips ◽  
Jmdc Pereira ◽  
Dcs Hutchison ◽  
H Baum
Keyword(s):  
Pancreatic Elastase ◽  
Porcine Pancreatic Elastase

scholarly journals Risk Assessment for Use of a Porcine Circovirus-Contaminated Reagent in a Barrier Maintained Rodent Colony

2020 ◽  
Vol 59 (5) ◽  
pp. 575-579
Author(s):  
Norman C Peterson ◽  
Aaron A Berlin
Keyword(s):  
Risk Assessment ◽  
Mouse Model ◽  
Cell Cultures ◽  
Direct Contact ◽  
Model Development ◽  
Porcine Circovirus ◽  
Laboratory Mice ◽  
Wild Mice ◽  
Pancreatic Elastase ◽  
Porcine Pancreatic Elastase

A proposal for the use of porcine pancreatic elastase (PPE) to develop a mouse model of pulmonary emphysema raised concerns about introducing contaminating porcine viruses into our barrier facility. Porcine Circovirus (PCV) is a known contaminant of vaccines and cell cultures that have been exposed to porcine-derived reagents. Endemic infection of PCV3 in laboratory mice has been reported, and some evidence supports natural PCV infection in wild mice. PPE samples from 2 different vendors tested positive for DNA from both PCV2 and 3. To allow model development with these reagents to proceed, we developed a protocol that would meet scientific objectives, minimize exposure of mice, and provide information on the potential for the virus to spread. Five d after BALB/c mice received intralaryngeal administration of PPE, lungs were harvested and analyzed for evidence of disease. Tissues from other major organs were submitted to test for disseminated PCV2 and 3 DNA. Similarly, tissues (including lungs) from direct contact nude sentinel mice were analyzed for the presence of the virus. To evaluate the possibility of endemic PCV2/3 infection, we also surveyed non-porcine reagent exposed mice on other studies. PCV2 and 3 was not detected in any of the tissues submitted. Although this study provided no evidence of infection and transmission of PCV2/3 from the contaminated PPE sample over the 5 d study, further work is needed to understand the risks and impact of introducing PCV contaminated cells or reagents into barrier maintained rodent colonies.

scholarly journals The Biochemical and Pathological Studies on Effects of Porcine Pancreatic Elastase in Experimental Atherosclerosis with Hyper-Cholesterolemia

1983 ◽  
Vol 11 (2) ◽  
pp. 365-374
Author(s):  
Shinzabro OHTAKE ◽  
Atsushi KOIDE ◽  
Hiroyuki SHIOJIRI ◽  
Kouichi KATAYAMA ◽  
Seiichi KOBAYASHI ◽  
...  
Keyword(s):  
Experimental Atherosclerosis ◽  
Pancreatic Elastase ◽  
Porcine Pancreatic Elastase

Rapid screening for deficiency of alpha 1-proteinase inhibitor.

1989 ◽  
Vol 35 (9) ◽  
pp. 1971-1975 ◽  
Author(s):  
C Lloyd ◽  
J Travis
Keyword(s):  
Human Plasma ◽  
Inhibitory Activity ◽  
Proteinase Inhibitor ◽  
Rapid Screening ◽  
Early Screening ◽  
Pancreatic Elastase ◽  
Low Concentrations ◽  
Normal Individuals ◽  
Porcine Pancreatic Elastase ◽  
Alpha 1 Antitrypsin

Abstract This rapid screening procedure for detection of low but functional elastase-inhibitory activity in human plasma is based on the fact that incubation of excess porcine pancreatic elastase (EC 3.4.21.36) with plasma results in formation of a complex with active alpha 1-proteinase inhibitor (alpha 1PI, also called alpha 1-antitrypsin). In normal individuals all of the elastase is complexed, leaving no free enzyme to hydrolyze the elastase substrate, and the reaction mixture remains clear. Because individuals homozygous for the Z allele have relatively low concentrations of alpha 1PI, their plasma cannot complex all of the elastase in the assay. The uncomplexed enzyme hydrolyzes the elastase-specific p-nitroanilide substrate, producing a yellow reaction mixture. Use of this simple assay for early screening of individuals for alpha 1PI deficiency may substantially decrease the number of untreated cases of familial emphysema, a disorder that develops as a result of a genetically derived proteinase-proteinase inhibitor imbalance.

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