scholarly journals 16S acetylcholinesterase of the extracellular matrix is assembled within mouse muscle cells in culture

1984 ◽  
Vol 217 (2) ◽  
pp. 377-381 ◽  
Author(s):  
N C Inestrosa
Keyword(s):  
Extracellular Matrix ◽  
Cell Surface ◽  
Membrane Permeability ◽  
Muscle Cells ◽  
Mouse Muscle ◽  
Intracellular Compartment ◽  
Ache Inhibitors ◽  
Irreversible Inhibition ◽  
Cells In Culture

The present paper examines where the extracellular-matrix (ECM) 16S acetylcholinesterase (AChE, EC 3.1.1.7) is assembled in muscle cells in culture. The existence of an internal pool of 16S AChE was detected by using AChE inhibitors of differing membrane permeability. After irreversible inhibition of all cellular esterase, the newly synthesized 16S form appears in an intracellular compartment and is only later detected on the cell surface. Results show that the ECM 16S AChE is assembled within muscle cells.

scholarly journals Interaction of the cytoskeletal framework with acetylcholine receptor on th surface of embryonic muscle cells in culture.

1982 ◽  
Vol 92 (1) ◽  
pp. 231-236 ◽  
Author(s):  
J Prives ◽  
A B Fulton ◽  
S Penman ◽  
M P Daniels ◽  
C N Christian
Keyword(s):  
Cell Surface ◽  
Internal Structure ◽  
Acetylcholine Receptor ◽  
Muscle Cells ◽  
Cells In Culture ◽  
Detergent Extraction ◽  
Embryonic Muscle ◽  
Alpha Bungarotoxin ◽  
Triton X

To monitor the interaction of cell surface acetylcholine (AcCho) receptors with the cytoskeleton, cultured muscle cells were labeled with radioactive or fluorescent alpha-bungarotoxin and extracted with Triton X-100, using conditions that preserve internal structure. A significant population of the AcCho receptors is retained on the skeletal framework remaining after detergent extraction. The skeleton organization responsible for restricting AcCho receptors to a patched region may also result in their retention after detergent extraction.

Elastase effect on the extracellular matrix of rat aortic smooth muscle cells in culture

1986 ◽  
Vol 45 (2) ◽  
pp. 105-117 ◽  
Author(s):  
Barbara Faris ◽  
Paul Toselli ◽  
Jennifer Kispert ◽  
B. Leslie Wolfe ◽  
Curtis A. Pratt ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Cells In Culture

Glucose uptake in human and animal muscle cells in culture

1990 ◽  
Vol 68 (2) ◽  
pp. 536-542 ◽  
Author(s):  
Vivian Sarabia ◽  
Toolsie Ramlal ◽  
Amira Klip
Keyword(s):  
Glucose Uptake ◽  
Muscle Cell ◽  
Muscle Cells ◽  
Human Muscle ◽  
Human Cells ◽  
Mouse Muscle ◽  
Cells In Culture ◽  
Maximal Stimulation ◽  
Hexose Uptake ◽  
Human Muscle Cells

Human muscle cells were grown in culture from satellite cells present in muscle biopsies and fusion-competent clones were identified. Hexose uptake was studied in fused myotubes of human muscle cells in culture and compared with hexose uptake in myotubes of the rat L6 and mouse C2C12 muscle cell lines. Uptake of 2-deoxyglucose was saturable and showed an apparent Km of about 1.5 mM in myotubes of all three cell types. The Vmax of uptake was about 6000 pmol/(min∙mg protein) in human cells, 4000 pmol/(min∙mg protein) in mouse C2C12 muscle cells, and 500 pmol/(min∙mg protein) in L6 cells. Hexose uptake was inhibited ~90% by cytochalasin B in human, rat, and mouse muscle cell cultures. Insulin stimulated 2-deoxyglucose uptake in all three cultures. The hormone also stimulated transport of 3-O-methylglucose. The sensitivity to insulin was higher in human and C2C12 mouse myotubes (half-maximal stimulation observed at 3.5 × 10−9 M) than in rat L6 myotubes (half-maximal stimulation observed at 2.5 × 10−8 M). However, insulin (10−6 M) stimulated hexose uptake to a larger extent (2.37-fold) in L6 than in either human (1.58-fold) or mouse (1.39-fold) myotubes. It is concluded that human muscle cells grown in culture display carrier-mediated glucose uptake, with qualitatively similar characteristics to those of other muscle cells, and that insulin stimulates hexose uptake in human cells. These cultures will be instrumental in the study of human insulin resistance and in investigations on the mechanism of action of antidiabetic drugs.Key words: glucose uptake, insulin action, primary muscle cultures, L6 cells, C2 cells.

Appearance of a class of cell-surface fucosyl-glycopeptides in differentiated muscle cells in culture

1980 ◽  
Vol 78 (2) ◽  
pp. 258-267 ◽  
Author(s):  
Mirella Marino ◽  
Giulio Cossu ◽  
Giovanni Neri ◽  
Mario Molinaro
Keyword(s):  
Cell Surface ◽  
Muscle Cells ◽  
Cells In Culture

Association of the synaptic form of acetylcholinesterase with extracellular matrix in cultured mouse muscle cells

Cell ◽  
1982 ◽  
Vol 29 (1) ◽  
pp. 71-79 ◽  
Author(s):  
Nibaldo C. Inestrosa ◽  
Laura Silberstein ◽  
Zach W. Hall
Keyword(s):  
Extracellular Matrix ◽  
Muscle Cells ◽  
Mouse Muscle
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