scholarly journals Neural 16S acetylcholinesterase is solubilized by heparin

1983 ◽  
Vol 215 (1) ◽  
pp. 201-204 ◽  
Author(s):  
J C Torres ◽  
M I Behrens ◽  
N C Inestrosa
Keyword(s):  
Sciatic Nerve ◽  
Esterase Activity ◽  
Ache Activity ◽  
Chondroitin Sulphate ◽  
Sedimentation Analysis ◽  
Rat Sciatic Nerve ◽  
Sulphated Glycosaminoglycans ◽  
Sulphated Glycosaminoglycan ◽  
Solubilizing Effect

The effect of heparin, a sulphated glycosaminoglycan, on the solubilization of rat sciatic-nerve acetylcholinesterase (acetylcholine acetylhydrolase; AChE; EC 3.1.1.7) was studied. It was found that heparin solubilized esterase activity from ligated nerves. Sedimentation analysis revealed this activity to be mainly the 16S form. Chondroitin sulphate did not solubilize AChE activity, and protamine eliminated the solubilizing effect. Our results suggest the involvement of sulphated glycosaminoglycans in the intra-axonal localization and transport of 16S AChE.

A histochemical study of sulphated glycosaminoglycans associated with the somites of the chick embryo

Development ◽  
1973 ◽  
Vol 29 (1) ◽  
pp. 197-208
Author(s):  
M. J. O'Hare
Keyword(s):  
Chick Embryo ◽  
Basement Membrane ◽  
Alcian Blue ◽  
Chondroitin Sulphate ◽  
Matrix Material ◽  
Paraxial Mesoderm ◽  
Membrane Material ◽  
Sulphated Glycosaminoglycans ◽  
Sulphated Glycosaminoglycan ◽  
Chondroitin Sulphate A

A histochemical analysis has been made of sulphated glycosaminoglycans (mucopolysaccharides) associated with chick embryo somites before and after the onset of overt cartilage differentiation. The sulphated glycosaminoglycans were distinguished and resolved into different types by the use of alcian blue at low pH and alcian blue ‘critical electrolyte concentration’ staining combined with hyaluronidase digestion. The newly formed somites are bounded on their dorsal, ventral, and medial surfaces by basement membrane material as they are delimited from the unsegmented paraxial mesoderm Such epithelial basement membrane material, which was first detected in association with the epiblast/mesoderm boundary in the stage-4 embryo, was found to contain a major chondroitin sulphate A/C fraction and a minor chondroitin sulphate B fraction. The notochord sheath contained similar sulphated glycosaminoglycans. Sulphated glycosaminoglycans were first detected between cells of the somite ‘core’ at stage 14 and were subsequently seen to accumulate around the cells of the developing sclerotome and later around cells of the dermatome; the myotome was devoid of such material at these stages (stage 14–20). These pre-cartilaginous sulphated glycosaminoglycans were also of the chondroitin sulphate A/C plus chondroitin sulphate B types. In contrast, the matrix material of newly forming vertebral cartilage, which was first seen in the anterior region of stage 21 embryos, was distinguished by its lack of a hyaluronidase-resistant sulphated glycosaminoglycan component, and therefore presumably contained only chondroitin sulphates A/C. Much later in development (after stage 33) small amounts of sulphated glycosaminoglycan with the staining properties of keratan sulphate were found in the perichordal and subperichondrial regions of the vertebral cartilage.

Dynamic Change of Prohibitin2 Expression in Rat Sciatic Nerve After Crush

2013 ◽  
Vol 33 (5) ◽  
pp. 689-698 ◽  
Author(s):  
Long Long ◽  
Yan Huang ◽  
Hao Wu ◽  
Wenkang Luan ◽  
Qi Zhang ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Dynamic Change ◽  
Rat Sciatic Nerve

Triptolide improves nerve regeneration and functional recovery following crush injury to rat sciatic nerve

2014 ◽  
Vol 561 ◽  
pp. 198-202 ◽  
Author(s):  
Yong-Guang Zhang ◽  
Qing-Song Sheng ◽  
Hong-Kun Wang ◽  
Li Lv ◽  
Jun Zhang ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Nerve Regeneration ◽  
Functional Recovery ◽  
Crush Injury ◽  
Rat Sciatic Nerve

Activity-dependent variations in conduction velocity of C fibers of rat sciatic nerve

1994 ◽  
Vol 19 (4) ◽  
pp. 427-431 ◽  
Author(s):  
Hyung-Cheul Shin ◽  
Yun-Lyul Lee ◽  
Hyeok-Yil Kwon ◽  
Hyoung Jin Park ◽  
Stephen A. Raymond
Keyword(s):  
Sciatic Nerve ◽  
Conduction Velocity ◽  
Rat Sciatic Nerve ◽  
C Fibers ◽  
Activity Dependent

Voltage-clamp analysis of nodes of Ranvier in regenerated rat sciatic nerve

1987 ◽  
Vol 409 (2) ◽  
pp. 227-235 ◽  
Author(s):  
T. Brismar ◽  
C. Hildebrand ◽  
S. Berglund
Keyword(s):  
Sciatic Nerve ◽  
Voltage Clamp ◽  
Nodes Of Ranvier ◽  
Rat Sciatic Nerve ◽  
Voltage Clamp Analysis
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