scholarly journals Characteristics of the desensitization and resensitization of the cyclic AMP-independent glycogenolytic response in rat liver cells

1982 ◽  
Vol 208 (2) ◽  
pp. 317-322 ◽  
Author(s):  
S Keppens ◽  
H De Wulf
Keyword(s):  
Cyclic Amp ◽  
Rat Liver ◽  
Isolated Hepatocytes ◽  
Liver Cells ◽  
Adrenergic Agonists ◽  
Tentative Model ◽  
Rat Liver Cells ◽  
Specific Antagonist ◽  
Dose Dependent

Vasopressin and alpha-adrenergic agonists were shown previously [Bréant, Keppens & De Wulf (1981) Biochem. J. 200, 509-514] to induce a heterologous, dose-dependent and receptor-mediated desensitization of the cyclic AMP-independent glycogenolytic response in isolated hepatocytes. The desensitized state of the hepatocytes can be preserved as long as the agonist is bound to its receptor. Conversely, washing the cells with a hormone-free buffer or displacement of the agonist from its receptor by a specific antagonist restores the responsiveness. The desensitization and its reversibility (i.e. resensitization) are obtained within minutes. The desensitization can be clearly elicited at temperatures as low as 5 degrees C, whereas the glycogenolytic response and the enhancement of the 45Ca flux are only obtained above 15 degrees C; the resensitization requires even higher temperatures. A tentative model is proposed to account for the observed effects.

MEDIATION BY CALCICALMODULIN AND CYCLIC AMP OF TUMOR PROMOTER-INDUCED DNA SYNTHESIS IN CALCIUM-DEPRIVED RAT LIVER CELLS

1982 ◽  
pp. 417-431 ◽  
Author(s):  
Alton L. Boynton ◽  
Leonard P. Kleine ◽  
Jon P. Durkin ◽  
James F. Whitfield ◽  
Alan Jones
Keyword(s):  
Cyclic Amp ◽  
Dna Synthesis ◽  
Rat Liver ◽  
Liver Cells ◽  
Tumor Promoter ◽  
Rat Liver Cells

scholarly journals Polyribosomes in isolated liver cells. Preparative procedures, effects of incubation and correlation with protein synthesis

1980 ◽  
Vol 186 (1) ◽  
pp. 35-45 ◽  
Author(s):  
A J Dickson ◽  
C I Pogson
Keyword(s):  
Protein Synthesis ◽  
Rat Liver ◽  
Isolated Hepatocytes ◽  
Liver Cells ◽  
Liver Protein ◽  
Isolated Cells ◽  
Isolated Liver Cells ◽  
Rat Liver Cells ◽  
Isolated Liver

Methods have been derived which permit the isolation of undergraded polyribosomes from isolated rat liver cells. Under the conditions used the polyribosome profile of hepatocytes immediately after isolation was essentially identical with that from intact liver. However, during incubation of cells in complex physiological media there was a progressive dissociation of polyribosomes. The addition of a variety of factors that produce reaggregation of polyribosomes in rat liver in vivo did not prevent dissociation during cell incubations. Although large polyribosomes were lost most rapidly, the albumin-synthesizing capacity of isolated cells was not selectively lost when compared with total protein synthesis. The significance of these results for the use of isolated hepatocytes in the study of liver protein synthesis is discussed.

REGULATION OF PROLIFERATION OF NORMAL AND NEOPLASTIC RAT LIVER CELLS BY CALCIUM AND CYCLIC AMP

1980 ◽  
Vol 349 (1) ◽  
pp. 294-311 ◽  
Author(s):  
S. H. H. Swierenga ◽  
J. F. Whitfield ◽  
A. L. Boynton ◽  
J. P. MacManus ◽  
R. H. Rixon ◽  
...  
Keyword(s):  
Cyclic Amp ◽  
Rat Liver ◽  
Liver Cells ◽  
Regulation Of Proliferation ◽  
Rat Liver Cells

Activation of protein kinase C sensitizes the cyclic AMP signalling system of T51B rat liver cells

1989 ◽  
Vol 1 (6) ◽  
pp. 617-625 ◽  
Author(s):  
Lise H. Aasheim ◽  
Leonard P. Kleine ◽  
Douglas J. Franks
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Cyclic Amp ◽  
Rat Liver ◽  
Liver Cells ◽  
Signalling System ◽  
Kinase C ◽  
Rat Liver Cells
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