scholarly journals An enzyme-linked immunoassay for the collagen cross-link pyridinoline

1982 ◽  
Vol 207 (3) ◽  
pp. 617-620 ◽  
Author(s):  
S P Robins
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Human Urine ◽  
Bovine Serum ◽  
Urine Samples ◽  
Cross Link ◽  
Human Urine Samples

An inhibition immunoassay method for the determination of pyridinoline was developed with the use of microtitre plates coated with a pyridinoline-gelatin conjugate and rabbit antisera directed against pyridinoline linked to bovine serum albumin. The sensitivity of the assay is about 2pmol of pyridinoline, and the presence of related pyridinium and lysine-derived compounds does not significantly interfere with the procedure. Its application to tissue and human urine samples is described.

Protein-protected red emittive copper nanoclusters as a fluorometric probe for highly sensitive biosensing of creatinine

2018 ◽  
Vol 10 (29) ◽  
pp. 3666-3674 ◽  
Author(s):  
Ramar Rajamanikandan ◽  
Malaichamy Ilanchelian
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Human Urine ◽  
Bovine Serum ◽  
Urine Samples ◽  
Copper Nanoclusters ◽  
Human Urine Samples ◽  
Fluorescent Nanoprobe ◽  
Highly Sensitive ◽  
Optical Recognition

We describe red emittive bovine serum albumin-modified copper nanoclusters (BSA-CuNCs) applied as a precise fluorescent nanoprobe for the optical recognition of creatinine in human urine samples.

RADIOIMMUNOASSAYS OF TETRAHYDROCORTISONE AND TETRAHYDROCORTISOL IN HUMAN URINE

1977 ◽  
Vol 86 (2) ◽  
pp. 369-379 ◽  
Author(s):  
H. Will ◽  
R. Aderjan ◽  
Th. Winkler ◽  
B. Penke ◽  
P. Vecsei
Keyword(s):  
New Zealand ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Human Urine ◽  
Bovine Serum ◽  
Secretion Rate ◽  
Cortisol Secretion ◽  
Cross Reactions ◽  
New Zealand Rabbits

ABSTRACT A simple radioimmunoassay for the determination of tetrahydrocortisone (THE)1), and tetrahydrocortisol (THF) as well as their 21-glucosiduronate conjugates is described. White New Zealand rabbits were immunized with THF- and THE-20-oxime-bovine serum-albumin conjugates. The resulting antisera proved to be very specific. Both allo-THE and allo-THF gave minimal cross-reactions; however, THE- and THF-21-glucosiduronate bind with the corresponding antibodies at a level of 100 %. The following types of estimations were introduced: a) Estimation of the sum of unconjugated THF and THF-21-glucosiduronate in highly diluted unprocessed human urine. b) A similar estimation of THE and THF-21-glucosiduronate as in a). c) Estimation of THF after β-glucuronidase treatment and dilution without further procedures. d) A similar estimation for THE as in c). THF and THE values correlate significantly with known parameters that characterize the cortisol production, such as cortisol secretion rate and the urinary corticoid (C21-α-ketolic-steroid) levels.

scholarly journals Electrochemical characterization and determination of carbamazepine as pharmaceutical standard and tablet content on gold electrode

2014 ◽  
Vol 68 (2) ◽  
pp. 207-212 ◽  
Author(s):  
Nemanja Trisovic ◽  
Bojan Bozic ◽  
Slobodan Petrovic ◽  
Svetlana Tadic ◽  
Milka Avramov-Ivic
Keyword(s):  
Cyclic Voltammetry ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Phosphate Buffer ◽  
Bovine Serum ◽  
Gold Electrode ◽  
Anticonvulsant Drug ◽  
Voltammetric Techniques ◽  
Anodic Behaviour

The anodic behaviour of carbamazepine (CBZ), an anticonvulsant drug, has been studied on gold electrode in 0.1 mol dm-3 phosphate buffer of pH 7.0 by using cyclic voltammetry. It has been found that the value of the oxidative current of pure CBZ at +0.90 V is a linear function of the concentration in a range from 1.0?10-7 to 1.0?10?4 mol dm?3. The detection of CBZ in the concentration of 1.0?10-8 mol dm-3 is among the lowest that have been reported for this drug using voltammetric techniques. CBZ as a content of tablet Galepsine? has been quantitatively determined. It has also been demonstrated that the modification of gold electrode with bovine serum albumin (BSA) results in a decrease of the oxidative peak current due to the binding of the drug to BSA.

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