scholarly journals Carbonic anhydrase C in white-skeletal-muscle tissue

1982 ◽  
Vol 205 (3) ◽  
pp. 559-566 ◽  
Author(s):  
W Siffert ◽  
G Gros

We investigated the activity of carbonic anhydrase in blood-free perfused white skeletal muscles of the rabbit. Carbonic anhydrase activities were measured in supernatants and in Triton extracts of the particulate fractions of white-skeletal-muscle homogenate by using a rapid-reaction stopped-flow apparatus equipped with a pH electrode. An average carbonic anhydrase concentration of about 0.5 microM was determined for white skeletal muscle. This concentration is about 1% of that inside the erythrocyte. Some 85% of the muscle enzyme was found in the homogenate supernatant, and only 15% appeared to be associated with membranes and organelles. White-skeletal-muscle carbonic anhydrase was characterized in terms of its Michaelis constant and catalytic-centre activity (turnover number) for CO2 and its inhibition constant towards ethoxzolamide. These properties were identical with those of the rabbit erythrocyte carbonic anhydrase C, suggesting that a type-C enzyme is present in white skeletal muscle. Affinity chromatography of muscle supernatant and of lysed erythrocytes showed that, whereas rabbit erythrocytes contain about equal amounts of carbonic anhydrase isoenzymes B and C, the B isoenzyme is practically absent from white skeletal muscle. Similarly, ethoxzolamide-inhibition curves suggested that white skeletal muscle contains no carbonic anhydrase A. It is concluded that white skeletal muscle contains essentially one carbonic anhydrase isoenzyme, the C form, most of which is probably of cytosolic origin.

1975 ◽  
Vol 53 (10) ◽  
pp. 1122-1127 ◽  
Author(s):  
J. A. C. Harrow ◽  
J. N. Singh ◽  
G. Jasmin ◽  
N. S. Dhalla

Cyclic AMP content, adenylate cyclase (EC 4.6.1.1) activity and phosphodiesterase I (EC 3.1.4.1) activity of the hind leg skeletal muscle and cardiac muscle in 60- and 150-day-old normal and myopathic (UM-X7.1) hamsters were examined. In 60-day-old myopathic animals, cardiac cyclic AMP levels were higher and phosphodiesterase I activity was lower, without any changes in the basal adenylate cyclase activity, whereas in 150-day-old myopathic hamsters, cardiac cyclic AMP and basal adenylate cyclase activity were lower, without any changes in the homogenate phosphodiesterase I activity. On the other hand, basal adenylate cyclase and phosphodiesterase I activities in the skeletal muscle homogenate from 60- and 150-day-old myopathic animals were not different from the normal values but the skeletal muscle cyclic AMP levels were significantly less in 60-day-old myopathic hamsters only. The plasma cyclic AMP levels in 60-day-old myopathic hamsters, unlike 150-day-old myopathic animals, were higher than the normal. Although these results reveal differences in myopathic cardiac and skeletal muscles, it is concluded that changes in adenylate cyclase – cyclic AMP system in myopathy are dependent upon the degree of disease.


Author(s):  
S.A. Moiseev

The question of physiological function variability is of great theoretical interest, since it is a part of the theory of human voluntary movement control. The skeletal muscle control system should probably have a mechanism to reduce or limit the range of its possible variations. Presumably, the organization of the motor system elements according to the principle of muscular synergy is of such a nature. The objective of the work is to study variations and signs of the coordinated bioelectric activity of skeletal muscles in one of the resulting archery phases. Materials and Methods. The study enrolled 5 highly qualified sportsmen (Master of Sport, International Master of Sport). Archers shot 10 series of 3 shots, target distance 18 m, indoors. Simultaneous recording of electrical activity of 12 skeletal muscles of the upper limb girdle and a 3D video sequence was made. The authors analyzed indicators of distribution, descriptive and variation statistics for grouped data. Multiple regression analysis was used to identify signs of consistent muscle activity. Results. Variability magnitudes, characterized by statistical parameters, established for the turn-off-peak characteristics of various muscles, did not have an explicit dependence. Muscles with relatively high scattering parameters in terms of the EMG average amplitude could have a small variation in the average number of EMG turns. The radial flexor of the left hand wrist was a part of muscular synergy in 90 % of cases, the anterior part of the left limb deltoid muscle – in 80 % of cases, the lower and upper beams of the right and left cowl muscle – in 70 % of cases. Other muscles under consideration were their part in less than 60 % of cases. Conclusion. The system of skeletal muscles that are actively involved in the resulting phases of precision movement can be controlled according to the mechanism of functional synergy formation, which probably helps to reduce the range of possible variations in the parameters of muscle electroactivity. Keywords: variability, archery, electromyography, coordination structure, muscle synergy. Вопрос вариативности физиологических функций представляет интерес в теоретическом плане, поскольку является частью теории управления произвольными движениями человека. Система управления скелетными мышцами, вероятно, должна иметь механизм, позволяющий сократить или ограничить диапазон возможных ее вариаций. Таковым, предположительно, является организация элементов моторной системы по принципу мышечных синергий. Цель работы – изучение вариаций и признаков согласованной биоэлектрической активности скелетных мышц в одной из результирующих фаз выстрела из лука. Материалы и методы. В исследованиях приняли участие 5 высококвалифицированных спортсменов (МС, МСМК). Лучники выполняли 10 серий по 3 выстрела с дистанции 18 м в крытом помещении. Производилась синхронная регистрация электрической активности 12 скелетных мышц верхнего плечевого пояса и 3D-видеоряда. Анализировались показатели распределения, описательной и вариационной статистики для сгруппированных данных. Для выявления признаков согласованной активности мышц применялся множественный регрессионный анализ. Результаты. Величины вариативности, характеризуемые статистическими параметрами, установленные для турн-аплитудных характеристик различных мышц, не имели явной зависимости. Мышцы, имеющие относительно высокие параметры разброса значений по показателю средней амплитуды ЭМГ, могли иметь небольшую вариативность среднего числа турнов ЭМГ. Лучевой сгибатель кисти левой руки являлся частью мышечной синергии в 90 % случаев, передняя часть дельтовидной мышцы левой конечности – в 80 %, нижние и верхние пучки трапециевидной мышцы правой и левой сторон – в 70 %. Другие исследуемые мышцы являлись их частью в менее чем 60 % случаев. Выводы. Управление системой скелетных мышц, принимающих активное участие в реализации одной из результирующих фаз точностного движения, может осуществляться по механизму образования функциональных синергий, что, вероятно, способствует снижению диапазона возможных вариаций параметров электроактивности мышц. Ключевые слова: вариативность, стрельба из лука, электромиография, координационная структура, мышечные синергии.


2021 ◽  
Vol 12 ◽  
pp. 204173142098133
Author(s):  
Juan M. Fernández-Costa ◽  
Xiomara Fernández-Garibay ◽  
Ferran Velasco-Mallorquí ◽  
Javier Ramón-Azcón

Muscular dystrophies are a group of highly disabling disorders that share degenerative muscle weakness and wasting as common symptoms. To date, there is not an effective cure for these diseases. In the last years, bioengineered tissues have emerged as powerful tools for preclinical studies. In this review, we summarize the recent technological advances in skeletal muscle tissue engineering. We identify several ground-breaking techniques to fabricate in vitro bioartificial muscles. Accumulating evidence shows that scaffold-based tissue engineering provides topographical cues that enhance the viability and maturation of skeletal muscle. Functional bioartificial muscles have been developed using human myoblasts. These tissues accurately responded to electrical and biological stimulation. Moreover, advanced drug screening tools can be fabricated integrating these tissues in electrical stimulation platforms. However, more work introducing patient-derived cells and integrating these tissues in microdevices is needed to promote the clinical translation of bioengineered skeletal muscle as preclinical tools for muscular dystrophies.


1986 ◽  
Vol 250 (5) ◽  
pp. E570-E575
Author(s):  
G. K. Grimditch ◽  
R. J. Barnard ◽  
S. A. Kaplan ◽  
E. Sternlicht

We examined the hypothesis that the exercise training-induced increase in skeletal muscle insulin sensitivity is mediated by adaptations in insulin binding to sarcolemmal (SL) insulin receptors. Insulin binding studies were performed on rat skeletal muscle SL isolated from control and trained rats. No significant differences were noted between groups in body weight or fat. An intravenous glucose tolerance test showed an increase in whole-body insulin sensitivity with training, and specific D-glucose transport studies on isolated SL vesicles indicated that this was due in part to adaptations in skeletal muscle. Enzyme marker analyses revealed no differences in yield, purity, or contamination of SL membranes between the two groups. Scatchard analyses indicated no significant differences in the number of insulin binding sites per milligram SL protein on the high-affinity (15.0 +/- 4.1 vs. 18.1 +/- 6.4 X 10(9)) or on the low-affinity portions (925 +/- 80 vs. 884 +/- 106 X 10(9)) of the curves. The association constants of the high-affinity (0.764 +/- 0.154 vs. 0.685 +/- 0.264 X 10(9) M-1) and of the low affinity sites (0.0096 +/- 0.0012 vs. 0.0102 +/- 0.0012 X 10(9) M-1) also were similar. These results do not support the hypothesis that the increased sensitivity to insulin after exercise training is due to changes in SL insulin receptor binding.


1978 ◽  
Vol 253 (8) ◽  
pp. 2540-2545 ◽  
Author(s):  
N.J. Hutson ◽  
B.S. Khatra ◽  
T.R. Soderling

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