scholarly journals Superoxide dismutase, glutathione peroxidase and catalase in developing rat brain

1982 ◽  
Vol 204 (2) ◽  
pp. 535-540 ◽  
Author(s):  
I Mavelli ◽  
A Rigo ◽  
R Federico ◽  
M R Ciriolo ◽  
G Rotilio
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Rat Brain ◽  
Rat Hepatocytes ◽  
Mitochondrial Fraction ◽  
Cytoplasmic Fraction ◽  
Mouse Ascites ◽  
Enzyme Pattern ◽  
Relative Deficiency ◽  
The Brain

The specific activities of Cu, Zn- and Mn-superoxide dismutases, of glutathione peroxidase and of catalase, the enzymes considered to be specifically involved in the defence of the cell against the partially reduced forms of oxygen, were determined as the function of postnatal age in the early (up to 60 days) period of rat brain development. The enzymes were assayed in the cytoplasmic fraction, in the crude mitochondrial fraction including peroxisomes, and in the mitochondria. The results show that the temporal changes of these enzymes cannot be correlated with each other, thus indicating that they do not concertedly parallel the increasing activity of aerobic brain metabolism during development. Specifically the cytoplasmic fraction shows a gradual increase of the Cu, Zn-superoxide dismutase activity with age, whereas the glutathione peroxidase activity is constant from birth. Furthermore the increase of the mitochondrial Mn-superoxide dismutase as a function of postnatal age is more remarkable than that of the cytoplasmic Cu, Zn-enzyme. Higher activities of catalase in adult animals are detectable only in the subcellular fraction containing peroxisomes, because of the modest catalase activity of the brain. These results indicate independent regulation of the expression of these enzyme activities in the process of brain differentiation and point to a relative deficiency of enzymic protection of the brain differentiation and point to a relative deficiency of enzymic protection of the brain against potentially toxic oxygen derivatives. This situation is similar to the pattern already described in the rat heart and in rat and mouse ascites-tumour cells, at variance with the much more efficient enzyme pattern present in rat hepatocytes.

Superoxide dismutase fromAscaris suum

Parasitology ◽  
1988 ◽  
Vol 97 (2) ◽  
pp. 345-353 ◽  
Author(s):  
M. Sanchez-Moreno ◽  
M. Monteoliva ◽  
A. Fatou ◽  
M. A. García-Ruiz
Keyword(s):  
Hydrogen Peroxide ◽  
Superoxide Dismutase ◽  
Mitochondrial Fraction ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Superoxide Dismutases ◽  
Cytoplasmic Fraction ◽  
Sephadex Column ◽  
Ammonium Sulphate Precipitation ◽  
Electrophoretic Patterns

SummaryThree superoxide dismutases (SOD) (EC 1.15.1.1) were detected in homogenates ofAscaris suum. Each of the three forms of SOD was purified by a sequence of multiple differential centrifugations, ammonium sulphate precipitation, ion-exchange chromatography and G-75 Sephadex column chromatography. The three forms of SOD were present in different cellular locations; one in the cytoplasmic fraction, sensitive to cyanide and hydrogen peroxide, and two in the mitochondrial fraction, one of which was cyanide sensitive. The SOD forms presented clear differences in their electrophoretic patterns. The sexual organs of females showed the highest SOD activities of all the tissues examined. The finding of such high levels of superoxide dismutase inA. suumreflects the importance of this enzyme in the metabolism of this helminth parasite.

Oophorectomy hinders antioxidant adaptation promoted by swimming in Wistar rats

2013 ◽  
Vol 38 (2) ◽  
pp. 148-153 ◽  
Author(s):  
Ulisvaldo Brunno de Oliveira Macedo ◽  
Rand Randall Martins ◽  
Francisco Paulo Freire Neto ◽  
Yonara Monique da Costa Oliveira ◽  
Aldo da Cunha Medeiros ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Wistar Rats ◽  
Antioxidant Defense ◽  
Hormone Replacement ◽  
Antioxidant Defense System ◽  
Control Group ◽  
Defense System ◽  
Antioxidant Parameters ◽  
The Brain

Oxidative stress is associated with postmenopause and is also responsible for various metabolic alterations. The redox imbalance observed during ovarian decline can be induced experimentally by bilateral ovariectomy in rats. In addition to hormone replacement, regular moderate physical exercise is indicated to prevent several common postmenopausal diseases. This study aimed to assess the effect of daily swimming on the antioxidant defense system of oophorectomized Wistar rats. Control and oophorectomized groups were submitted to 1 h of daily swimming for 90 days. Levels of lipid peroxidation and glutathione content and the activities of superoxide dismutase enzyme and glutathione peroxidase in erythrocytes, liver, and brain were assessed every 30 days. The control group exhibited lower lipoperoxidation that was associated with a significant increase in superoxide dismutase enzyme activity, glutathione peroxidase activity, and glutathione content in erythrocytes and liver; however, swimming did not cause changes in antioxidant parameters in the brain over time. The oophorectomized group showed no antioxidant adaptation to daily swimming and had greater oxidative damage in the liver and blood. Our results suggest that ovariectomy hinders antioxidant adaptation in Wistar rats submitted to daily swimming.

NGF restores decrease in catalase activity and increases superoxide dismutase and glutathione peroxidase activity in the brain of aged rats

1992 ◽  
Vol 12 (3) ◽  
pp. 177-181 ◽  
Author(s):  
Giuseppe Nisticò ◽  
Maria Rosa Ciriolo ◽  
Kayahan Fiskin ◽  
Michelangelo Iannone ◽  
Angelo de Martino ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Catalase Activity ◽  
Aged Rats ◽  
Glutathione Peroxidase Activity ◽  
The Brain
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