scholarly journals Effect of 4-hydroxypyrazole on tryptophan and formate metabolism in isolated rat liver cells

1982 ◽  
Vol 204 (1) ◽  
pp. 307-312 ◽  
Author(s):  
J S Cook ◽  
C I Pogson
Keyword(s):  
Liver Cells ◽  
Isolated Rat Liver ◽  
Formate Oxidation ◽  
Isolated Liver Cells ◽  
Rat Liver Cells ◽  
Isolated Liver ◽  
Isolated Rat ◽  
Formate Metabolism ◽  
In Cells

1. 4-Hydroxypyrazole inhibits flux through tryptophan 2.3-dioxygenase in cells. The inhibition is apparently non-competitive with Ki = 0.15 mM. 2. Hydroxypyrazole inhibits the oxidation of formate to CO2 in liver cells. 3. Glycollate, which generates H2O2, stimulates formate oxidation. This process is inhibited by 4-hydroxypyrazole. 4. Methionine stimulates formate oxidation in cells and this stimulation is insensitive to 4-hydroxypyrazole. 5. It is concluded that, in freshly isolated liver cells, formate oxidation proceeds by a pathway involving catalase. In vivo, or when methionine is added to cell incubations, the pathway of oxidation involves tetrahydrofolate, and is insensitive to catalase inhibitors. 6. Methionine at physiological concentrations inhibits the activity of tryptophan 2,3-dioxygenase in isolated liver cells.

scholarly journals Polyribosomes in isolated liver cells. Preparative procedures, effects of incubation and correlation with protein synthesis

1980 ◽  
Vol 186 (1) ◽  
pp. 35-45 ◽  
Author(s):  
A J Dickson ◽  
C I Pogson
Keyword(s):  
Protein Synthesis ◽  
Rat Liver ◽  
Isolated Hepatocytes ◽  
Liver Cells ◽  
Liver Protein ◽  
Isolated Cells ◽  
Isolated Liver Cells ◽  
Rat Liver Cells ◽  
Isolated Liver

Methods have been derived which permit the isolation of undergraded polyribosomes from isolated rat liver cells. Under the conditions used the polyribosome profile of hepatocytes immediately after isolation was essentially identical with that from intact liver. However, during incubation of cells in complex physiological media there was a progressive dissociation of polyribosomes. The addition of a variety of factors that produce reaggregation of polyribosomes in rat liver in vivo did not prevent dissociation during cell incubations. Although large polyribosomes were lost most rapidly, the albumin-synthesizing capacity of isolated cells was not selectively lost when compared with total protein synthesis. The significance of these results for the use of isolated hepatocytes in the study of liver protein synthesis is discussed.

Antiketogenic effect of glucose per se in vivo in man and in vitro in isolated rat liver cells

Metabolism ◽  
1986 ◽  
Vol 35 (7) ◽  
pp. 608-613 ◽  
Author(s):  
J.P. Riou ◽  
M. Beylot ◽  
M. Laville ◽  
L. De Parscau ◽  
J. Delinger ◽  
...  
Keyword(s):  
Rat Liver ◽  
Liver Cells ◽  
Isolated Rat Liver ◽  
Rat Liver Cells ◽  
Per Se ◽  
Effect Of Glucose ◽  
Isolated Rat

Vasopressin promotes the metabolism of near-physiological concentration of glutamine in isolated rat liver cells

1984 ◽  
Vol 4 (2) ◽  
pp. 171-176 ◽  
Author(s):  
Guy Martin ◽  
Gabriel Baverel
Keyword(s):  
Rat Liver ◽  
Liver Cells ◽  
Hepatic Gluconeogenesis ◽  
Physiological Concentration ◽  
Isolated Rat Liver ◽  
Rat Liver Cells ◽  
Isolated Rat

In isolated rat liver cells, vasopressin, like glucagon, promotes the metabolism of glutamine used at nearphysiological concentration (1 mM). These findings indicate that, in vivo, both hormones might participate in the control of hepatic gluconeogenesis and ureogenesis from glutamine.

Gene expression of syndecans and betaglycan in isolated rat liver cells

1996 ◽  
Vol 285 (1) ◽  
pp. 11-16 ◽  
Author(s):  
O. H. Weiner ◽  
M. Zoremba ◽  
A. M. Gressner
Keyword(s):  
Gene Expression ◽  
Rat Liver ◽  
Liver Cells ◽  
Isolated Rat Liver ◽  
Rat Liver Cells ◽  
Isolated Rat
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