scholarly journals Foetomaternal relationships of serum bile acid pattern estimated by high-pressure liquid chromatography

1982 ◽  
Vol 204 (1) ◽  
pp. 141-145 ◽  
Author(s):  
S Itoh ◽  
S Onishi ◽  
K Isobe ◽  
M Manabe ◽  
K Inukai
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Bile Acid ◽  
Bile Acids ◽  
High Pressure Liquid Chromatography ◽  
Placental Transfer ◽  
Umbilical Vein ◽  
Maternal Blood ◽  
Serum Bile Acid ◽  
Umbilical Blood

The bile acid patterns in the maternal and umbilical vein and artery serum samples were analysed by a two-step chromatographic method involving group separation by piperidinohydroxypropyl-Sephadex LH-20 and high-pressure liquid chromatography using immobilized 3 alpha-hydroxy steroid dehydrogenase. Glycochenodeoxycholate predominates in the maternal blood and taurochenodeoxycholate in the umbilical blood. In cases where a free bile acid was detected in the maternal blood, the same bile acid was also demonstrated in the corresponding cord blood. The concentrations of taurocholate and taurochenodeoxycholate were found to be significantly higher in the umbilical artery than in the corresponding umbilical vein. Our data suggest that there is a bidirectional placental transfer of free bile acids and that there is a transfer of taurine-conjugated primary bile acids from the foetus to the mother.

scholarly journals Assay of free and glycine- and taurine-conjugated bile acids in serum by high-pressure liquid chromatography by using post-column reaction after group separation

1982 ◽  
Vol 204 (1) ◽  
pp. 135-139 ◽  
Author(s):  
S Onishi ◽  
S Itoh ◽  
Y Ishida
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Bile Acid ◽  
Bile Acids ◽  
High Pressure Liquid Chromatography ◽  
Exchange Chromatography ◽  
Group Separation ◽  
Serum Samples ◽  
Hydroxy Steroid ◽  
Steroid Dehydrogenase

An accurate and sensitive method that involves the group separations of serum bile acids (i.e. free and glycine- and taurine-conjugated bile acid fractions) by ion-exchange chromatography on piperidinohydroxypropyl-Sephadex LH-20 is described. Each group was then analysed by high-pressure liquid chromatography by using the post-column reaction technique with immobilized 3 alpha-hydroxy steroid dehydrogenase. The bile acid patterns in the umbilical venous serum samples were analysed by this method. Taurochenodeoxycholate predominated in the umbilical cord.

Bile Acid Synthesis in the Developing Sheep Liver

1973 ◽  
Vol 45 (3) ◽  
pp. 403-406
Author(s):  
R. A. Smallwood ◽  
P. Jablonski ◽  
J. McK. Watts
Keyword(s):  
Bile Acid ◽  
Bile Acids ◽  
Placental Transfer ◽  
Umbilical Vein ◽  
Acid Synthesis ◽  
Taurocholic Acid ◽  
Secondary Bile Acid ◽  
Radioactive Label ◽  
Sheep Liver ◽  
Conjugated Bile Acids

1. [14C]Cholesterol was administered intravenously via the umbilical vein to foetal sheep in the latter half of gestation, and the incorporation of radioactive label into foetal bile acids was assessed. 2. After 4 days, 0·5–2% of the radioactive label was found in foetal bile. Seventy to eighty per cent of the radioactive label in foetal bile was present as [14C]taurocholic acid and [14C]taurochenodeoxycholic acid. The remainder was [14C]cholesterol. No radioactive label was found in taurodeoxycholic acid, or in any of the glycine-conjugated bile acids. 3. It is concluded that the foetal sheep liver in the second half of gestation synthesizes taurocholic acid and taurochenodeoxycholic acid. However, the secondary bile acid taurodeoxycholic acid and the glycine-conjugated bile acids, present in foetal bile, have been acquired by placental transfer from the mother.

Sign in / Sign up

Export Citation Format

Share Document