scholarly journals The subcellular localization of the β-galactoside-binding protein of rat lung

1982 ◽  
Vol 204 (1) ◽  
pp. 97-102 ◽  
Author(s):  
G L Sanford ◽  
L D Davis ◽  
J T Powell
Keyword(s):  
Subcellular Localization ◽  
Immunoglobulin G ◽  
Specific Binding ◽  
Binding Protein ◽  
Rat Lung ◽  
Lectin Activity ◽  
Intracellular Location ◽  
Immature Rat ◽  
Adult Rat ◽  
Rat Lungs

The subcellular localization of the beta-galactoside-binding protein, or lectin, from rat lung was investigated by the specific binding of anti-lectin immunoglobulin G to subcellular fractions. We used both adult and immature (12-day-old) rats; the immature rat lungs have an 8-10-fold greater concentration than adult rat lungs [Powell & Whitney (1980) Biochem. J. 188, 1-8]. In both groups of animals we observed greater specific binding of anti-lectin immunoglobulin G to intracellular membrane (mitochondrial and microsomal fractions) than to plasma membranes. Pre-incubation of membrane fractions with lactose resulted in a marked diminution of anti-lectin immunoglobulin G binding. In the adult rat lung most (approx. 80%) of the lectin activity was membrane-associated. In the immature rat lung only approx. 30% of the lectin activity was membrane associated and most of the beta-galactoside-binding protein appeared to be a soluble cytoplasmic component. The rat lung beta-galactoside-binding protein appeared to have a broad but predominantly intracellular location, being associated with membranes through one of its galactoside-binding sites.

scholarly journals Rat lung lectin synthesis, degradation and activation. Developmental regulation and modulation by dexamethasone

1987 ◽  
Vol 245 (3) ◽  
pp. 683-690 ◽  
Author(s):  
L B Clerch ◽  
P L Whitney ◽  
D Massaro
Keyword(s):  
Binding Protein ◽  
Developmental Regulation ◽  
Fold Increase ◽  
Rat Lung ◽  
Absolute Rate ◽  
Lectin Activity ◽  
Developmentally Regulated ◽  
Rat Pups ◽  
The Absolute

Soluble lectins are widely distributed cell-agglutinating proteins. Their activity is developmentally regulated in several tissues, including the lung, but virtually nothing is known about the mechanisms of the developmental regulation or the turnover of these proteins. We studied mechanisms that might be responsible for the developmentally regulated changes in the activity of a lectin (beta-galactoside-binding protein) found in the lung, and determined if its activity or turnover could be modulated by treatment of rat pups with a glucocorticosteroid hormone (dexamethasone). Our studies on the activity and turnover of the lectin indicated that the peak of lectin activity (units/mg of protein) that occurred at age 12 days appeared to be brought about by two means: an increase in the activity of the lectin molecule itself (units/micrograms of lectin) that occurred at age 8 days, and 1.5-fold increase in the absolute rate of lectin synthesis at age 11 days. The decline in lectin activity was associated with a decrease in its rate of synthesis, return to the baseline extent of activation, and an increased rate of degradation. Treatment of rat pups with dexamethasone diminished the peak of lectin activity (units/mg of protein) by about 25%. This effect of dexamethasone was due, at least in part, to the complete prevention of activation of the lectin molecule (units/micrograms of lectin) and a premature increase in the rate of lectin degradation. Perhaps the normal fall in lectin activity after age 11 days is caused by mechanisms induced by the increase in serum corticosteroid that occurs at that age.

Detection and characterization of a heat and acid stable progesterone binding protein in the rat lung

1984 ◽  
Vol 104 (4_Supplb) ◽  
pp. S91-S92
Author(s):  
G. DAXENBICHLER ◽  
E. H. MOSER
Keyword(s):  
Binding Protein ◽  
Rat Lung ◽  
Acid Stable

Intestinal Transport and Processing of Immunoglobulin G in the Neonatal and Adult Rat

Neonatology ◽  
1995 ◽  
Vol 67 (4) ◽  
pp. 254-263 ◽  
Author(s):  
N. Benlounes ◽  
R. Chedid ◽  
F. Thuillier ◽  
J.F. Desjeux ◽  
F. Rousselet ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Intestinal Transport ◽  
Adult Rat

Immunolocalization of 67 kDa elastin-binding protein in perinatal rat lungs

1992 ◽  
Vol 268 (2) ◽  
pp. 277-281 ◽  
Author(s):  
Kojiro Wasano ◽  
Yasuhiro Hirakawa ◽  
Keiichiro Nakamura
Keyword(s):  
Binding Protein ◽  
Rat Lungs ◽  
Elastin Binding Protein
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