scholarly journals Analysis and separation of natural and synthetic mixtures of uroporphyrins by high-pressure liquid chromatography

1982 ◽  
Vol 203 (2) ◽  
pp. 515-517 ◽  
Author(s):  
Anthony H. Jackson ◽  
K. R. Nagaraja Rao ◽  
Sydney G. Smith
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
High Pressure Liquid Chromatography ◽  
Methyl Esters ◽  
New Method ◽  
Preparative Isolation ◽  
Naturally Occurring ◽  
Natural And Synthetic

A new method for the quantitative analysis of mixtures of the methyl esters of uroporphyrins I and III was developed; this can be applied both to the analysis of naturally occurring uroporphyrins and also to their semi-preparative isolation.

Quantitative Analysis of Photochemical Reactions Utilizing High Pressure Liquid Chromatography: Linear Polyenes Related to Vitamin A

1979 ◽  
Vol 2 (8) ◽  
pp. 1205-1218 ◽  
Author(s):  
Walter H. Waddell ◽  
Patricia M. Dawson ◽  
Daniel L. Hopkins ◽  
Karen L. Rach ◽  
Motokazu Uemura ◽  
...  
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Vitamin A ◽  
High Pressure Liquid Chromatography ◽  
Photochemical Reactions ◽  
Linear Polyenes

Distribution of Aflatoxins in Some Samples of Peanuts

1976 ◽  
Vol 59 (4) ◽  
pp. 941-944
Author(s):  
Vincent P Diprossimo
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Analysis Data ◽  
Sample Analysis ◽  
Naturally Occurring

Abstract Naturally occurring C aflatoxins were found at approximately 20 times the level of B aflatoxins in one lot of roasted, blanched peanuts. Official methods, as well as high-pressure liquid chromatography, were used to confirm this finding. Additional routine sample analysis data of raw and roasted peanuts revealed that this finding is not so unusual as formerly thought. It was found that 9.8% of the raw peanut samples contained higher levels of G than B aflatoxin; 4.9% containing 2–8 times more G than B aflatoxins. In a smaller sampling of roasted peanuts, 28% of the contaminated peanuts contained more G than B aflatoxins.

Sensitive and selective assay for adenosine using high-pressure liquid chromatography with fluorometry

1983 ◽  
Vol 245 (5) ◽  
pp. H887-H890 ◽  
Author(s):  
M. K. Jacobson ◽  
L. M. Hemingway ◽  
T. A. Farrell ◽  
C. E. Jones
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Direct Detection ◽  
Peak Height ◽  
Uv Absorption ◽  
New Method ◽  
Fluorometric Method ◽  
Primary Advantage ◽  
Fluorescence Peak

A new method for quantitation of adenosine was tested in canine myocardial extracts. The method involves incubation of the extract with chloroacetaldehyde to form the fluorescing adenosine derivative 1, N6-ethenoadenosine. The ethenoadenosine is separated by high-pressure liquid chromatography (HPLC) and quantitated by fluorometry. Experiments demonstrated that 1) the method is selective for adenosine, 2) fluorescence peak height is linearly related to the quantity of ethenoadenosine, and 3) adenosine in the extract is quantitatively converted to ethenoadenosine by the incubation procedure. Also, within the range of adenosine concentrations seen in five extracts, estimates of myocardial adenosine content with the fluorometric method were nearly identical to those using the more routine technique of HPLC with direct detection by ultraviolet (UV) absorption. A primary advantage of the fluorometric method is its greater sensitivity. As little as 0.50 pmol on the column could be quantitated by fluorescence, compared with approximately 20 pmol with UV absorption. Because of the greater sensitivity, the fluorometric method should be more easily applied to samples with smaller adenosine concentrations.

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