scholarly journals Identity of ligandin in rat testis and liver

1982 ◽  
Vol 203 (1) ◽  
pp. 193-199 ◽  
Author(s):  
K A Eidne ◽  
R E Kirsch
Keyword(s):  
Reduced Glutathione ◽  
Sodium Dodecyl ◽  
Standard Technique ◽  
Electrophoretic Analysis ◽  
Immunoaffinity Chromatography ◽  
Multiple Forms ◽  
Glutathione S Transferase ◽  
Closely Related Species ◽  
Exclusion Chromatography ◽  
Molecular Exclusion Chromatography

1. One of the main problems in the field of multifunctional proteins such as ligandin is the possibility that multiple forms and isoproteins may exist. Because liver ligandin [GSH (reduced glutathione) S-transferase B] consists of equal amounts of Ya (22 000 Da) and Yc (25 000 Da) subunits, and testis ligandin, prepared by the standard technique of anion-exchange and molecular-exclusion chromatography, contains more Yc subunit than Ya, it has been claimed that testis and liver ligandin are different entities. 2. We purified testis ligandin by immunoaffinity chromatography and have obtained a product identical with liver ligandin (Yc = Ya). This suggests that the differences previously described may be due to contamination of testis ligandin by a closely related species. In fact sodium dodecyl sulphate/polyacrylamide-gel-electrophoretic analysis of testis GSH S-transferases separated by CM-cellulose chromatography showed that GSH S-transferase AA, present in large amounts, migrated in the same region as Yc subunit. 3. Testis ligandin prepared by the standard technique was similar to that reported [Bhargava, Ohmi, Listowsky & Arias (1980) J. Biol. Chem. 255, 724-727] and contained more Yc subunit than Ya. CM-cellulose chromatography of this ‘pure’ preparation revealed significant amounts of GSH S-transferase AA migrating as Yc subunit, in addition to ligandin consisting of equal amounts of Ya and Yc subunits. 4. Our studies show that testis ligandin is identical with liver ligandin. Previously described differences are due to a contaminant identified as GSH S-transferase AA.

Acrosin: immunochemical demonstration of multiple forms generated from bovine and human proacrosin

1983 ◽  
Vol 61 (9) ◽  
pp. 989-995 ◽  
Author(s):  
John S. Elce ◽  
Elise J. McIntyre
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Rabbit Antiserum ◽  
Immunoaffinity Chromatography ◽  
Relative Mass ◽  
Multiple Forms ◽  
Western Blots ◽  
Specific Immunoglobulin ◽  
Single Form ◽  
Bovine Spermatozoa

A rabbit antiserum was prepared against purified bovine spermatozoal acrosin (EC 3.4.21.10), and the specific immunoglobulin G (IgG) was isolated by immunoaffinity chromatography. This IgG was shown to be monospecific for acrosin by rocket immunoelectrophoresis and by Western blotting of sodium dodecyl sulfate – polyacrylamide gels onto nitrocellulose sheets, followed by indirect immunodetection. In extracts of bovine spermatozoa prepared in the presence of 50 mM benzamidine, a single form of acrosin was detected, having a relative mass (Mr) of 48 000, which is presumed to be proacrosin. At least four further intermediate forms of acrosin were detectable in extracts prepared in the absence of benzamidine and in the various column eluates, having Mr values of 47 000, 44 000, 42 000, and 40 000, while the final product of the purification had a Mr of 37 500. The rabbit antibovine acrosin antiserum reacted also with human acrosin on Western blots. In this way, human proacrosin was found to have a Mr of 50 000 and to be convertible into intermediate forms of Mr 48 500, 44 500, 40 500, and 37 500, while the final product had a Mr of 35 500.

scholarly journals Neuronal and Oxidative Damage in the Catfish Brain Alleviated After Mucuna Seed Extract Treatment

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Mukherjee Mainak ◽  
Moniruzzaman Mahammed ◽  
Kumar Saheli ◽  
Das Debjit ◽  
Chakraborty Suman Bhusan
Keyword(s):  
Methanol Extract ◽  
Reduced Glutathione ◽  
Sodium Dodecyl ◽  
Seed Extract ◽  
Mucuna Pruriens ◽  
Dependent Manner ◽  
Enzymatic Antioxidants ◽  
Glutathione S Transferase ◽  
Brain Physiology ◽  
Significant Difference

The neurodegenerative activity of a synthetic detergent sodium dodecyl sulphate (SDS) on brain physiology in Indian native catfish Heteropneustes fossilis and the efficacy of methanol extract of Mucuna pruriens seeds for alleviating such effects were demonstrated. Fish (n=36, 2 replicates) were exposed to SDS (2.75 mg/l) for 0 (control), 15 and 30 days. After 30-days treatment, methanol extract of Mucuna seed was injected for continuous seven days and sampling was done on each alternate odd days (1, 3, 5 and 7 days). Levels of different enzymatic and non-enzymatic antioxidants, Na+-K+-ATPase, acetylcholine esterase; monoamine oxidase; nitric oxide were measured in H. fossilis brain tissue. 30- days treatment with SDS caused significant decrease in reduced glutathione, catalase, superoxide dismutase, glutathione S-transferase, while glutathione reductase, malondialdehyde level increased significantly (P less than 0.05). Administration of Mucuna seed extract (15.5 mg/kg body weight) was found to restore the neurological activity and reduce stress in a time-dependent manner as the biochemical and neurological parameters in fish after 7-day extract administration showed no significant difference (P>0.05) compared to those in control without SDS treatment, except for GST and GPx which were unable to return to the basal level.

The Kidney and Fibrinolysis

1970 ◽  
Vol 24 (01/02) ◽  
pp. 026-032 ◽  
Author(s):  
N. A Marsh
Keyword(s):  
Molecular Weight ◽  
Plasminogen Activator ◽  
Enzyme System ◽  
Normal Animal ◽  
Fibrinolytic Enzyme ◽  
The Other ◽  
Exclusion Chromatography ◽  
Normal Urine ◽  
Renal Origin ◽  
Molecular Exclusion Chromatography

SummaryMolecular exclusion chromatography was performed on samples of urine from normal and aminonucleoside nephrotic rats. Normal urine contained 2 peaks of urokinase activity, one having a molecular weight of 22,000 and the other around 200,000. Nephrotic urine contained three peaks of activity with MW’s 126,000, 60,000 and 30,000. Plasma activator determined from euglobulin precipitate had a MW. in excess of 200,000. The results indicate that in the normal animal, plasma plasminogen activator does not escape into the urine in substantial quantities but under the conditions of extreme proteinuria there may be some loss through the kidney. The alteration in urokinase output in nephrotic animals indicates a greatly disordered renal fibrinolytic enzyme system.The findings of this study largely support the hypothesis that plasma plasminogen activator of renal origin and urinary plasminogen activator (urokinase) are different molecular species.

scholarly journals Dietary Black Seed Effects on Growth Performance, Proximate Composition, Antioxidant and Histo-Biochemical Parameters of a Culturable Fish, Rohu (Labeo rohita)

Animals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 48
Author(s):  
Maria Latif ◽  
Mehwish Faheem ◽  
Asmatullah ◽  
Seyed Hossein Hoseinifar ◽  
Hien Van Doan
Keyword(s):  
Growth Performance ◽  
Proximate Composition ◽  
Biochemical Parameters ◽  
Reduced Glutathione ◽  
Labeo Rohita ◽  
Nigella Sativa ◽  
Growth Promoter ◽  
Feeding Trial ◽  
Glutathione S Transferase ◽  
Black Seed

This feeding trial was conducted to investigate the effects of dietary black seed (Nigella sativa) supplementation on the growth performance, muscles proximate composition, antioxidant and histo-biochemical parameters of rohu (Labeo rohita). Fingerlings (8.503 ± 0.009 g) were fed on 0.0%, 1% and 2.5% black seed supplemented diets for 28 days. Fish sampling was done on the 7th, 14th, 21st and 28th day of experiment. The results of the present study indicated that black seed supplementation significantly increased growth performance and muscles protein contents of rohu over un-supplemented ones. Lipid peroxidation levels significantly decreased in all the studied tissues (liver, gills, kidney and brain) of black seed fed rohu, whereas the antioxidant enzymes (catalase, glutathione-S-transferase, glutathione peroxidase and reduced glutathione) activities were increased in all the studied tissues of black seed supplemented rohu at each sampling day. The hepatic-nephric marker enzymes levels were decreased for black seed fed rohu. The present study showed that tested black seed levels are safe for rohu. Black seed is cheaply available in local markets of Pakistan; therefore, based on the results of the present study, it is suggested that black seed has potential to be used as natural growth promoter and antioxidant in the diet of rohu.

scholarly journals Jatropha Neopauciflora Pax Latex Exhibits Wound-Healing Effect in Normal and Diabetic Mice

2021 ◽  
Vol 26 ◽  
pp. 2515690X2098676
Author(s):  
Ana Bertha Hernandez-Hernandez ◽  
Francisco Javier Alarcon-Aguilar ◽  
Mario Garcia-Lorenzana ◽  
Marco Aurelio Rodriguez-Monroy ◽  
Maria Margarita Canales-Martinez
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Wound Healing Process ◽  
Diabetic Mice ◽  
Wound Area ◽  
Oral Infections ◽  
Patients With Diabetes ◽  
Exclusion Chromatography ◽  
Wound Healing Effect ◽  
Molecular Exclusion Chromatography

Jatropha neopauciflora is an endemic species of Mexico. Its latex is used to treat wounds, scarring, oral infections, and loose teeth. To date, there are no studies that validate at a morphological level a wound-healing use in diabetes. The present research aimed to evaluate the wound-healing capacity of the latex of J. neopauciflora in the skin of healthy and streptozotocin-induced diabetic mice. Also, a chemical analysis of the latex through molecular exclusion chromatography and HPLC were performed. Male mice ( Mus musculus) of 7-week-old CD1 strain were used. Groups of healthy and diabetic mice were formed. A longitudinal cut of 1 cm was performed on the depilated skin. All treatments were topically applied to the wound area twice a day for ten days. At the end of the experiments, the skin sections were obtained from the wound area and stained with Hematoxylin-Eosin. Then we counted the number of active fibroblasts in all the experimental groups. In normal mice, the latex accelerated the wound-healing process and decreased the number of active fibroblasts, similarly to Recoveron. In diabetic mice, the latex and Recoveron increased the number of active fibroblasts. In normal and diabetic mice, a thin and orderly epidermis was observed. Molecular exclusion chromatography exhibited 58 fractions, 14 of which were subjected to HPLC, to detect catechin, a flavonoid with antioxidant, antimicrobial, and anti-inflammatory properties. J. neopauciflora latex can be useful for wound treatment in patients with diabetes mellitus because it accelerates and promotes the wound-healing process.

scholarly journals Multiple Forms of Human Glutathione S-Transferase and Their Affinity for Bilirubin

1975 ◽  
Vol 60 (1) ◽  
pp. 153-161 ◽  
Author(s):  
Kazuaki KAMISAKA ◽  
William H. HABIG ◽  
Jeanne N. KETLEY ◽  
Irwin M. ARIAS ◽  
William B. JAKOBY
Keyword(s):  
Multiple Forms ◽  
Glutathione S Transferase

Application of a flow system with laser polarimetric automatic detection for molecular exclusion chromatography of carbohydrates

Sugar Tech ◽  
2006 ◽  
Vol 8 (4) ◽  
pp. 229-232 ◽  
Author(s):  
Silvio Naranjo ◽  
Teresa Ceperob ◽  
Victor Fajerb ◽  
Carlos W. Rodriguez ◽  
William Morab ◽  
...  
Keyword(s):  
Flow System ◽  
Automatic Detection ◽  
Exclusion Chromatography ◽  
Molecular Exclusion Chromatography

scholarly journals Effect of ellagic acid on growth and some antioxidant parameters in scaly carp (Cyprinus carpio)

2021 ◽  
Vol 38 (3) ◽  
pp. 337-343
Author(s):  
Tuncay Eksen ◽  
Serpil Mişe Yonar
Keyword(s):  
Ellagic Acid ◽  
Specific Growth ◽  
Reduced Glutathione ◽  
Live Weight ◽  
Control Group ◽  
Glutathione S Transferase ◽  
Relative Growth ◽  
Carp Cyprinus Carpio ◽  
Antioxidant Parameters ◽  
Liver And Kidney

In the present study, it was investigated the effects of various levels of dietary ellagic acid on growth performance and antioxidant status in scaly carp (Cyprinus carpio). Fish were fed with the control diet and three different experimental diets containing three graded levels of ellagic acid (50, 100 and 200 mg kg-1 diet) for 60 days. On 30th and 60th days of experiment, the growth performance [live weight gain, relative growth and specific growth rate] and oxidant/antioxidant parameters [malondialdehyde level, catalase and glutathione-S-transferase activities and reduced glutathione level] were analysed. There was no statistically significant difference in the live weight gain, relative growth and specific growth rates of the control and ellagic acid treated groups (p > 0.05). When compared to the control group, the liver and kidney malondialdehyde levels of ellagic acid treated groups were significantly decreased (p < 0.05). The liver and kidney catalase and glutathione-S-transferase activities and reduced glutathione levels of ellagic acid treated groups were significantly increased when compared to the control group (p < 0.05). It was concluded that ellagic acid can be used as an antioxidant in fish.

Ras GTPase-activating protein physically associates with mitogenically active phospholipids

1991 ◽  
Vol 11 (5) ◽  
pp. 2785-2793
Author(s):  
M H Tsai ◽  
M Roudebush ◽  
S Dobrowolski ◽  
C L Yu ◽  
J B Gibbs ◽  
...  
Keyword(s):  
Mixed Micelles ◽  
Arachidic Acid ◽  
Physical Interaction ◽  
Magnesium Ions ◽  
Gtpase Activating Protein ◽  
Elution Time ◽  
Ras Gtpase ◽  
Exclusion Chromatography ◽  
Tight Association ◽  
Molecular Exclusion Chromatography

The physical interaction between GTPase-activating protein (GAP) and lipids has been characterized by two separate analyses. First, bacterially synthesized GAP molecules were found to associate with detergent-mixed micelles containing arachidonic but not with those containing arachidic acid. This association was detected by a faster elution time during molecular exclusion chromatography. Second, GAP molecules within a crude cellular lysate were specifically retained by a column on which certain lipids had been immobilized. The lipids able to retain GAP on such columns were identical to those which were shown previously to be most active in blocking GAP activity. The association between lipids and GAP was dependent upon magnesium ions. Lipids unable to inhibit GAP activity were also unable to physically associate with GAP. The tight association of GAP with these lipids was predicted by and helps to rationalize their ability to inhibit GAP activity.

Preliminary Treatment of Urinary Proteins Improves Electrophoretic Analysis and Immunodetection

1992 ◽  
Vol 38 (6) ◽  
pp. 860-863 ◽  
Author(s):  
J M Verdier ◽  
B Dussol ◽  
P Dupuy ◽  
Y Berland ◽  
J C Dagorn
Keyword(s):  
Protein Pattern ◽  
Protein A ◽  
Sodium Dodecyl ◽  
Complex Mixture ◽  
Electrophoretic Analysis ◽  
Renal Physiology ◽  
Preliminary Treatment ◽  
Urinary Proteins ◽  
Simple Method ◽  
Electrophoretic Migration

Abstract Analysis of urinary protein composition is an important tool in studies on renal physiology and physiopathology. Urine is, however, a complex mixture containing, besides protein, a variety of compounds such as salts, peptides, oligosaccharides, and glycosaminoglycans. Some of these compounds interfere with the electrophoretic migration of protein in sodium dodecyl sulfate-polyacrylamide gels and prevent correct analysis of the protein pattern. We describe a simple method for extracting urinary proteins that considerably improves their electrophoretic migration and subsequent immunodetection. This treatment involves ammonium sulfate fractionations (for precipitating proteins), EDTA (for inhibiting protein aggregation), and HCl hydrolysis (for removing glycosylaminoglycans). Recovery during extraction was found to be almost quantitative for total protein and three representative proteins: albumin, alpha 1-glycoprotein acid, and beta 2-microglobulin.

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