The crystal structure of an inverting glycoside hydrolase family 9 exo-β-D-glucosaminidase and the design of glycosynthase

2016 ◽  
Vol 473 (4) ◽  
pp. 463-472 ◽  
Author(s):  
Yuji Honda ◽  
Sachiko Arai ◽  
Kentaro Suzuki ◽  
Motomitsu Kitaoka ◽  
Shinya Fushinobu

The crystal structure of an inverting exo-β-D-glucosaminidase from glycoside hydrolase family 9 was determined. This is the first description of the structure of an exo-type enzyme from this family. A glycosynthase was produced from this enzyme through saturation mutagenesis.

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Vol 15 (6) ◽  
pp. e0231513 ◽  
Author(s):  
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