Bombyx mori prothoracicostatic peptide receptor is allosterically activated via a Gαi/o-protein-biased signalling cascade by Drosophila sex peptide

2015 ◽  
Vol 466 (2) ◽  
pp. 391-400 ◽  
Author(s):  
Xiaobai He ◽  
Jiashu Zang ◽  
Huipeng Yang ◽  
Haishan Huang ◽  
Ying Shi ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Receptor Internalization ◽  
Peptide Receptor ◽  
Sex Peptide ◽  
Signalling Cascade

We here demonstrated that Bombyx mori prothoracicostatic peptide was allosterically activated by Drosophila sex peptide leading to Gαi-independent inhibition of cAMP, while its activation resulted in Ca2+ mobilization and kurtz-dependent receptor internalization in response to the endogenous agonist prothoracicostatic peptide.

scholarly journals Bombyx prothoracicostatic peptides activate the sex peptide receptor to regulate ecdysteroid biosynthesis

2010 ◽  
Vol 107 (5) ◽  
pp. 2060-2065 ◽  
Author(s):  
Naoki Yamanaka ◽  
Yue-Jin Hua ◽  
Ladislav Roller ◽  
Ivana Spalovská-Valachová ◽  
Akira Mizoguchi ◽  
...  
Keyword(s):  
Expression System ◽  
Neurosecretory Cells ◽  
Neuroendocrine System ◽  
Prothoracic Gland ◽  
Heterologous Expression System ◽  
Peptide Receptor ◽  
Sex Peptide ◽  
Ecdysteroid Biosynthesis ◽  
High Level ◽  
G Protein Coupled

Insect molting and metamorphosis are induced by steroid hormones named ecdysteroids, whose production is regulated by various neuropeptides. We cloned the gene and analyzed the expression of the prothoracicostatic peptide, a unique neuropeptide shown to suppress the production of ecdysteroids in the prothoracic gland of the silkworm, Bombyx mori. We also characterized a Bombyx G protein-coupled receptor, which has previously been identified as an ortholog of the Drosophila sex peptide receptor, as a functional prothoracicostatic peptide receptor. This receptor responded specifically to the prothoracicostatic peptides when examined using a heterologous expression system. The receptor was highly expressed in the prothoracic gland on the day before each larval and pupal ecdysis, when prothoracicostatic peptides are synthesized at a high level in the epiproctodeal glands. These results suggest that the sex peptide receptor functions as a prothoracicostatic peptide receptor in Bombyx and that the peripheral neurosecretory cells as well as the central neuroendocrine system play stage-specific roles in regulating ecdysteroidogenesis.

Influence of the silencing sex-peptide receptor on Bactrocera dorsalis adults and offspring by feeding with ds-spr

2015 ◽  
Vol 18 (3) ◽  
pp. 477-481 ◽  
Author(s):  
Wenping Zheng ◽  
Yaru Liu ◽  
Weiwei Zheng ◽  
Yunli Xiao ◽  
Hongyu Zhang
Keyword(s):  
Bactrocera Dorsalis ◽  
Peptide Receptor ◽  
Sex Peptide

scholarly journals MIPs are ancestral ligands for the sex peptide receptor

2010 ◽  
Vol 107 (14) ◽  
pp. 6520-6525 ◽  
Author(s):  
Y.-J. Kim ◽  
K. Bartalska ◽  
N. Audsley ◽  
N. Yamanaka ◽  
N. Yapici ◽  
...  
Keyword(s):  
Peptide Receptor ◽  
Sex Peptide

scholarly journals Changes in Female Drosophila Sleep following Mating Are Mediated by SPSN-SAG Neurons

2016 ◽  
Vol 31 (6) ◽  
pp. 551-567 ◽  
Author(s):  
David S Garbe ◽  
Abigail S Vigderman ◽  
Emilia Moscato ◽  
Abigail E. Dove ◽  
Christopher G. Vecsey ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Egg Production ◽  
Behavioral Responses ◽  
Mating Status ◽  
Peptide Receptor ◽  
Dietary Preference ◽  
Sex Peptide ◽  
Central Brain ◽  
Sleep Sex ◽  
Sensorimotor Processes

Female Drosophila melanogaster, like many other organisms, exhibit different behavioral repertoires after mating with a male. These postmating responses (PMRs) include increased egg production and laying, increased rejection behavior (avoiding further male advances), decreased longevity, altered gustation and decreased sleep. Sex Peptide (SP), a protein transferred from the male during copulation, is largely responsible for many of these behavioral responses, and acts through a specific circuit to induce rejection behavior and alter dietary preference. However, less is known about the mechanisms and neurons that influence sleep in mated females. In this study, we investigated postmating changes in female sleep across strains and ages and on different media, and report that these changes are robust and relatively consistent under a variety of conditions. We find that female sleep is reduced by male-derived SP acting through the canonical sex peptide receptor (SPR) within the same neurons responsible for altering other PMRs. This circuit includes the SPSN-SAG neurons, whose silencing by DREADD induces postmating behaviors including sleep. Our data are consistent with the idea that mating status is communicated to the central brain through a common circuit that diverges in higher brain centers to modify a collection of postmating sensorimotor processes.

scholarly journals A neuropeptide signaling system that rapidly enforces paternity in the Aedes aegypti mosquito

2017 ◽  
Author(s):  
Laura B. Duvall ◽  
Nipun S. Basrur ◽  
Henrik Molina ◽  
Conor J. McMeniman ◽  
Leslie B. Vosshall
Keyword(s):  
Aedes Aegypti ◽  
Egg Production ◽  
Seminal Fluid ◽  
Ejaculatory Duct ◽  
Accessory Gland ◽  
Biologically Active ◽  
Wild Type ◽  
Peptide Receptor ◽  
Sex Peptide ◽  
Asian Tiger

AbstractFemale Dengue and Zika vector mosquitoes (Aedes aegypti) generally mate once, with sperm from this male fertilizing all eggs produced in her lifetime. Here we implicate HP-I, an Aedes- and male-specific neuropeptide transferred to females, and its cognate receptor in the female, NPYLR1, in rapid enforcement of paternity. HP-I mutant males were ineffective in enforcing paternity when a second male was given access to the female within 1 hour. NPYLR1 mutant females produced mixed paternity offspring at high frequency. Synthetic HP-I injected into wild-type virgins reduced successful matings, but had no effect on NPYLR1 mutant females. Asian tiger mosquito (Ae. albopictus) HP-I potently activated Ae. aegypti NPYLR1. Invasive Ae. albopictus males are known to copulate with and sterilize Ae. aegypti females, and cross-species transfer of HP-I may contribute to this phenomenon. This neuropeptide system promotes rapid paternity enforcement within Ae. aegypti, but may promote local extinction in areas where they compete with Ae. albopictus.One Sentence SummaryAedes-specific peptide rapidly enforces paternityTextAe. aegypti females typically mate only once with one male in their lifetime, a behavior known as “monandry” (1). This single mating event provisions the female with sufficient sperm to fertilize the >500 eggs she will produce during her ∼4-6 week lifespan in the laboratory (2). Successful mating is capable of inducing lifetime refractoriness to subsequent insemination by other males, enforcing the paternity of the first male (3-5). In other species, males use diverse strategies to assure the paternity of their offspring, for instance physical barriers such as mating plugs found in mice (6) and Anopheline mosquitoes (7), and anti-aphrodisiac pheromones used by Drosophila melanogaster males to tag female flies as non-virgin (8). Another widely used strategy in insects is the transfer of biologically active male seminal proteins, produced by the male accessory gland and secreted into the ejaculatory duct along with sperm during insemination, to affect the sexual receptivity of the female (3, 9-13). Perhaps the best-characterized male seminal fluid protein in insects is the Drosophila fly sex peptide (11), which acts on the sex peptide receptor in the female to suppress receptivity and trigger egg production (12). Drosophila sex peptide receptor mutant females will readily remate with multiple males, and wild-type females that mate with sex peptide mutant males remain sexually receptive.

Myoinhibiting peptides are the ancestral ligands of the promiscuous Drosophila sex peptide receptor

2010 ◽  
Vol 67 (20) ◽  
pp. 3511-3522 ◽  
Author(s):  
Jeroen Poels ◽  
Tom Van Loy ◽  
Hans Peter Vandersmissen ◽  
Boris Van Hiel ◽  
Sofie Van Soest ◽  
...  
Keyword(s):  
Peptide Receptor ◽  
Sex Peptide
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