The expression of the tumour suppressor HBP1 is down-regulated by growth factors via the PI3K/PKB/FOXO pathway

2014 ◽  
Vol 460 (1) ◽  
pp. 25-36 ◽  
Author(s):  
Alexandra Coomans de Brachène ◽  
Emeline Bollaert ◽  
Astrid Eijkelenboom ◽  
Audrey de Rocca Serra ◽  
Kristan E. van der Vos ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Growth Factors ◽  
Binding Site ◽  
Tumour Suppressor

We identified HBP1 as a gene that is down-regulated by growth factors through the PI3K/PKB/FOXO pathway. A FOXO-binding site was characterized in the HBP1 promoter. HBP1 knockdown was enough to enhance cell proliferation downstream of growth factors.

Beta cell proliferation and growth factors

1999 ◽  
Vol 77 (1) ◽  
pp. 62-66 ◽  
Author(s):  
Jens Høiriis Nielsen ◽  
C. Svensson ◽  
Elisabeth Douglas Galsgaard ◽  
Annette Møldrup ◽  
Nils Billestrup
Keyword(s):  
Cell Proliferation ◽  
Growth Factors ◽  
Beta Cell ◽  
Beta Cell Proliferation

Role of Growth Factors in the Control of Leukemic Cell Proliferation

1994 ◽  
Vol 13 (sup1) ◽  
pp. 35-37 ◽  
Author(s):  
Christian Chabannon ◽  
Patrice Mannoni
Keyword(s):  
Cell Proliferation ◽  
Growth Factors ◽  
Leukemic Cell

Steroid-induced cell proliferation in vivo is associated with increased c-myc proto-oncogene transcript abundance

Development ◽  
1988 ◽  
Vol 104 (1) ◽  
pp. 87-95
Author(s):  
S.A. Rempel ◽  
R.N. Johnston
Keyword(s):  
Cell Proliferation ◽  
Growth Factors ◽  
Steroid Hormones ◽  
Steroid Hormone ◽  
Normal Liver ◽  
Transcript Abundance ◽  
Chicken Oviduct ◽  
Massive Cell ◽  
In Cells

Enhanced c-myc transcript abundance has been observed in a variety of human malignancies, in normal liver tissue induced to proliferate in vivo by partial hepatectomy and in cells in culture induced to proliferate with the addition of protein hormones and growth factors. Little is known, however, about the expression of cellular proto-oncogenes in cells induced to proliferate in vivo by steroid hormones. Experiments reported here indicate that when cells of the immature chicken oviduct are induced to undergo rapid in vivo proliferation by application of the estrogen hormone 17 beta-estradiol, the onset of this proliferation is associated with a rapid, large, and transient increase in c-myc transcript abundance. When estrogen is administered to chickens in which the oviduct has already differentiated, neither massive cell proliferation nor large increases in c-myc transcript abundance are induced. We conclude that the abundance of c-myc transcripts in vivo correlates well with the degree of cell proliferation induced by steroid hormone.

435 REDUCTION OF CELL CYCLE LENGTH AND INCREASE IN S-PHASE BY GROWTH FACTORS IN PIG FETAL FIBROBLASTS

2010 ◽  
Vol 22 (1) ◽  
pp. 374
Author(s):  
S. Waghmare ◽  
B. Mir
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Growth Factors ◽  
Growth Factor ◽  
Cycle Length ◽  
S Phase ◽  
Proliferation Rate ◽  
Fetal Fibroblasts ◽  
Cell Cycle Length ◽  
Number Of Cells

Gene targeting in primary somatic cells is inefficient compared with embryonic stem cells. This is because of a slow rate of cell proliferation, fewer cells in S-phase at a given time point under normal culture conditions, and low rate of homologous recombination. Homologous recombination occurs mainly in late S-phase and increase in gene targeting efficiency has been reported in S-phase synchronized cells in bovine and rhesus macaque fetal fibroblasts. In this study we tested several growth factors: platelet-derived growth factor (PDGF), tumor necrosis factor a (TNFα), epidermal growth factor (EGF), fibroblast growth factor (FGF), transforming growth factor β1 (TGFβ1), insulin-like growth factor 1 (ILGF-1) and insulin-like growth factor II (ILGF-II) individually and in various combinations to see the effect on cell proliferation rate. Each experimental set consisted of 3 replicates. TGFβ1-, ILGF1-, ILGFII-, and FGF-treated cells grew very slowly compared with untreated cells. However, a combination of 3 growth factors: PDGF (15 ng mL-1), EGF (50 ng mL-1) and TNFa (100 pg mL-1), herein referred to as the cocktail, accelerated cell proliferation rate and reduced cell cycle length on average from 24.5 ± 0.2 to 20.4 ± 0.5 h with no significant change in number of cells in S-phase. Further, cells grown in the presence of the cocktail showed changes in morphology. The cells became spindle-shaped and occupied less surface area per cell compared with untreated cells. Importantly, cocktail-treated cells maintained a normal karyotype without any chromosomal abnormality. Thymidine has been used successfully to block various cell types in S-phase but it failed to synchronize these cells in S-phase in the concentration range of 2 to 10 mM for 24 to 48 h. However, serum starvation (0.2% fetal bovine serum) for 48 h blocked the cell proliferation rate effectively and synchronized cells in G0 phase (80-82% cells). After releasing from the block, cells were grown in the absence or presence of cocktail and cell cycle analysis was done at different time points by flow cytometry. Each time point was repeated 3 times. We observed the maximum number of cells in S-phase at 22 to 23 h (61.33% ± 7.77 in cocktail-treated cells v. 41.7% ± 3.28 in untreated cells). In summary, the cocktail-treated cells showed changes in cell morphology, higher proliferation rate, reduction in cell cycle length by 16.7%, and maximum percentage of cells in S-phase following serum starvation but maintained normal karyotypes. This high proliferation rate, reduction in cell cycle length, and maximum number of cells in S-phase should be very helpful in increasing the efficiency of gene-targeting in pig fetal fibroblasts.

scholarly journals miR-192-5p suppresses the progression of lung cancer bone metastasis by targeting TRIM44

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Peng Zou ◽  
Menghai Zhu ◽  
Chong Lian ◽  
Jiaqiang Wang ◽  
Zhiquan Chen ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Proliferation ◽  
Bone Metastasis ◽  
Binding Site ◽  
Cancer Cells ◽  
Significant Role ◽  
Research Group ◽  
Luciferase Activity ◽  
Migration And Invasion

AbstractLung cancer is the leading cause of cancer-related deaths worldwide, with 50–70% of patients suffering from bone metastasis. Accumulating evidence has demonstrated that miRNAs are involved in cell proliferation, migration, and invasion in malignancy, such as lung cancer bone metastasis. In the present study, we demonstrated that reduced miR-192-5p and increased TRIM44 levels were associated with the proliferation, migration and invasion of lung cancer. Furthermore, the potential functions of miR-192-5p were explored in A549 and NCI-H1299 cells. We found that miR-192-5p upregulation suppressed tumour behaviours in lung cancer cells. To further investigate whether miR-192-5p is associated with TRIM44, we used TargetScan software to predict the binding site between miR-192-5p and TRIM44. Luciferase activity assays were performed to verify this prediction. In addition, the significant role of miR-192-5p in negatively regulating TRIM44 expression was manifested by our research group. our results suggest that miR-192-5p inhibited the proliferation, migration and invasion of lung cancer through TRIM44.

scholarly journals Wnt signaling promotes proliferation and stemness regulation of spermatogonial stem/progenitor cells

Reproduction ◽  
2009 ◽  
Vol 138 (1) ◽  
pp. 151-162 ◽  
Author(s):  
Nady Golestaneh ◽  
Elspeth Beauchamp ◽  
Shannon Fallen ◽  
Maria Kokkinaki ◽  
Aykut Üren ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Migration ◽  
Growth Factors ◽  
Progenitor Cells ◽  
Cell Fate ◽  
Morphological Changes ◽  
Embryonic Stem ◽  
Large Family ◽  
Self Renewal ◽  
Fate Determination

Spermatogonial stem cells (SSCs) self-renew throughout life to produce progenitor cells that are able to differentiate into spermatozoa. However, the mechanisms underlying the cell fate determination between self-renewal and differentiation have not yet been delineated. Culture conditions and growth factors essential for self-renewal and proliferation of mouse SSCs have been investigated, but no information is available related to growth factors that affect fate determination of human spermatogonia. Wnts form a large family of secreted glycoproteins, the members of which are involved in cell proliferation, differentiation, organogenesis, and cell migration. Here, we show that Wnts and their receptors Fzs are expressed in mouse spermatogonia and in the C18-4 SSC line. We demonstrate that WNT3A induces cell proliferation, morphological changes, and cell migration in C18-4 cells. Furthermore, we show that β-catenin is activated during testis development in 21-day-old mice. In addition, our study demonstrates that WNT3A sustained adult human embryonic stem (ES)-like cells derived from human germ cells in an undifferentiated stage, expressing essential human ES cell transcription factors. These results demonstrate for the first time that Wnt/β-catenin pathways, especially WNT3A, may play an important role in the regulation of mouse and human spermatogonia.

Growth factors involved in aqueous humour-induced lens cell proliferation

2009 ◽  
Vol 27 (1) ◽  
pp. 50-62 ◽  
Author(s):  
Laxmi Iyengar ◽  
Bramilla Patkunanathan ◽  
John W. Mcavoy ◽  
Frank J. Lovicu
Keyword(s):  
Cell Proliferation ◽  
Growth Factors ◽  
Aqueous Humour ◽  
Lens Cell
Sign in / Sign up

Export Citation Format

Share Document