Native oligomerization determines the mode of action and biological activities of human cathelicidin LL-37

2013 ◽  
Vol 457 (2) ◽  
pp. 263-275 ◽  
Author(s):  
Daniela Xhindoli ◽  
Sabrina Pacor ◽  
Filomena Guida ◽  
Nikolinka Antcheva ◽  
Alessandro Tossi
Keyword(s):  
Self Assembly ◽  
Mode Of Action ◽  
Biological Activities ◽  
Biological Membranes ◽  
Innate Immune ◽  
Cross Linking ◽  
Covalent Dimerization

Covalent dimerization and photochemical cross-linking were used to study self-assembly in the multifunctional innate immune peptide LL-37, to determine how this evolved trait affects its interaction with biological membranes and how it influences its biological activities.

scholarly journals Diketopiperazine Gels: New Horizons from the Self-Assembly of Cyclic Dipeptides

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3376
Author(s):  
Marco Scarel ◽  
Silvia Marchesan
Keyword(s):  
Drug Discovery ◽  
Enzymatic Hydrolysis ◽  
Self Assembly ◽  
Biological Activities ◽  
Functional Materials ◽  
The Self ◽  
Cyclic Dipeptides ◽  
New Horizons ◽  
Concise Review

Cyclodipeptides (CDPs) or 2,5-diketopiperazines (DKPs) can exert a variety of biological activities and display pronounced resistance against enzymatic hydrolysis as well as a propensity towards self-assembly into gels, relative to the linear-dipeptide counterparts. They have attracted great interest in a variety of fields spanning from functional materials to drug discovery. This concise review will analyze the latest advancements in their synthesis, self-assembly into gels, and their more innovative applications.

scholarly journals Toward Physicochemical and Rheological Characterization of Different Injectable Hyaluronic Acid Dermal Fillers Cross-Linked with Polyethylene Glycol Diglycidyl Ether

Polymers ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 948
Author(s):  
Nicola Zerbinati ◽  
Sabrina Sommatis ◽  
Cristina Maccario ◽  
Maria Chiara Capillo ◽  
Giulia Grimaldi ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Polyethylene Glycol ◽  
Biological Activities ◽  
Diglycidyl Ether ◽  
Dermal Filler ◽  
Cross Linking ◽  
Matrix Structure ◽  
Dermal Fillers ◽  
Rheological Characterization

(1) Background: Injectable hyaluronic acid (HA) dermal fillers are used to restore volume, hydration and skin tone in aesthetic medicine. HA fillers differ from each other due to their cross-linking technologies, with the aim to increase mechanical and biological activities. One of the most recent and promising cross-linkers is polyethylene glycol diglycidyl ether (PEGDE), used by the company Matex Lab S.p.A., (Brindisi, Italy) to create the HA dermal filler PEGDE family. Over the last few years, several studies have been performed to investigate the biocompatibility and biodegradability of these formulations, but little information is available regarding their matrix structure, rheological and physicochemical properties related to their cross-linking technologies, the HA content or the degree of cross-linking. (2) Methods: Seven different injectable HA hydrogels were subjected to optical microscopic examination, cohesivity evaluation and rheological characterization in order to investigate their behavior. (3) Results: The analyzed cross-linked dermal fillers showed a fibrous “spiderweb-like” matrix structure, with each medical device presenting different and peculiar rheological features. Except for HA non cross-linked hydrogel 18 mg/mL, all showed an elastic and cohesive profile. (4) Conclusions: The comparative analysis with other literature works makes a preliminary characterization of these injectable medical devices possible.

A conformational tweak for enhanced cellular internalization, photobleaching resistance and prolonged imaging efficacy

2020 ◽  
Vol 56 (94) ◽  
pp. 14861-14864
Author(s):  
Niranjan Meher ◽  
Anil Parsram Bidkar ◽  
Debasish Barman ◽  
Siddhartha Sankar Ghosh ◽  
Parameswar Krishnan Iyer
Keyword(s):  
Self Assembly ◽  
Biological Activities ◽  
Cellular Internalization ◽  
Molecular Systems ◽  
Simple Strategy ◽  
Fine Tune

A simple strategy of conformational manipulation has been unveiled to fine-tune the photophysical and supramolecular self-assembly properties of small molecular systems, which subsequently regulates their biological activities.

Multilayer composite beads constructed via layer-by-layer self-assembly for lysozyme controlled release

RSC Advances ◽  
2014 ◽  
Vol 4 (46) ◽  
pp. 24369-24376 ◽  
Author(s):  
Jiemin Zhao ◽  
Xiaoping Wang ◽  
Yanshen Kuang ◽  
Yufeng Zhang ◽  
Xiaowen Shi ◽  
...  
Keyword(s):  
Controlled Release ◽  
Self Assembly ◽  
Cross Linking ◽  
Layer By Layer ◽  
Multilayer Composite ◽  
Composite Beads ◽  
Assembly Technique ◽  
Positively Charged

Alginate (ALG)–lysozyme (LZ) beads were fabricated by a cross-linking process. Negatively charged ALG and positively charged LZ were alternately deposited on the positively charged ALG–LZ beads via a layer-by-layer (LBL) self-assembly technique.

scholarly journals Preparation and Cross-Linking of All-Acrylamide Diblock Copolymer Nano-Objects via Polymerization-Induced Self-Assembly in Aqueous Solution

2017 ◽  
Vol 50 (4) ◽  
pp. 1482-1493 ◽  
Author(s):  
Sarah J. Byard ◽  
Mark Williams ◽  
Beulah E. McKenzie ◽  
Adam Blanazs ◽  
Steven P. Armes
Keyword(s):  
Aqueous Solution ◽  
Diblock Copolymer ◽  
Self Assembly ◽  
Cross Linking

scholarly journals Chemically Cross-Linked Graphene Oxide as a Selective Layer on Electrospun Polyvinyl Alcohol Nanofiber Membrane for Nanofiltration Application

Nanomaterials ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 2867
Author(s):  
Myoung Jun Park ◽  
Grace M. Nisola ◽  
Dong Han Seo ◽  
Chen Wang ◽  
Sherub Phuntsho ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Polyvinyl Alcohol ◽  
Self Assembly ◽  
Water Flux ◽  
Composite Membranes ◽  
Cross Linking ◽  
Support Surface ◽  
Eosin Y ◽  
Selective Layer ◽  
Assembly Technique

Graphene oxide (GO) nanosheets were utilized as a selective layer on a highly porous polyvinyl alcohol (PVA) nanofiber support via a pressure-assisted self-assembly technique to synthesize composite nanofiltration membranes. The GO layer was rendered stable by cross-linking the nanosheets (GO-to-GO) and by linking them onto the support surface (GO-to-PVA) using glutaraldehyde (GA). The amounts of GO and GA deposited on the PVA substrate were varied to determine the optimum nanofiltration membrane both in terms of water flux and salt rejection performances. The successful GA cross-linking of GO interlayers and GO-PVA via acetalization was confirmed by FTIR and XPS analyses, which corroborated with other characterization results from contact angle and zeta potential measurements. Morphologies of the most effective membrane (CGOPVA-50) featured a defect-free GA cross-linked GO layer with a thickness of ~67 nm. The best solute rejections of the CGOPVA-50 membrane were 91.01% for Na2SO4 (20 mM), 98.12% for Eosin Y (10 mg/L), 76.92% for Methylene blue (10 mg/L), and 49.62% for NaCl (20 mM). These findings may provide one of the promising approaches in synthesizing mechanically stable GO-based thin-film composite membranes that are effective for solute separation via nanofiltration.

scholarly journals On demand MyD88 oligomerization is controlled by IRAK4 during Myddosome signaling

2020 ◽  
Author(s):  
Rafael Deliz-Aguirre ◽  
Fakun Cao ◽  
Fenja H. U. Gerpott ◽  
Nichanok Auevechanichkul ◽  
Mariam Chupanova ◽  
...  
Keyword(s):  
Self Assembly ◽  
Intracellular Signaling ◽  
Innate Immune ◽  
Live Cells ◽  
Signaling Proteins ◽  
Protein Oligomerization ◽  
Downstream Signaling ◽  
Oligomeric Complex ◽  
Mechanistic Basis ◽  
Cell Signaling Pathways

AbstractA recurring feature of innate immune receptor signaling is the self-assembly of signaling proteins into oligomeric complexes. The Myddosome is an oligomeric complex that is required to transmit inflammatory signals from TLR/IL1Rs and consists of MyD88 and IRAK family kinases. However, the molecular basis for how Myddosome proteins self-assemble and regulate intracellular signaling remains poorly understood. Here, we developed a novel assay to analyze the spatiotemporal dynamics of IL1R and Myddosome signaling in live cells. We found that MyD88 oligomerization is inducible and initially reversible. Moreover, the formation of larger, stable oligomers consisting of more than 4 MyD88s triggers the sequential recruitment of IRAK4 and IRAK1. Notably, genetic knockout of IRAK4 enhanced MyD88 oligomerization, indicating that IRAK4 controls MyD88 oligomer size and growth. MyD88 oligomer size thus functions as a physical threshold to trigger downstream signaling. These results provide a mechanistic basis for how protein oligomerization might function in cell signaling pathways.

Self-assembly of g-C3N4-based 3D aerogel induced by N-modified carbon dots for enhanced photocatalytic hydrogen production

2021 ◽  
Author(s):  
Pu Xiao-Long ◽  
yang xuechun ◽  
Shan-Shan Liang ◽  
Wenzhong Wang ◽  
Jing-Tai Zhao ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Self Assembly ◽  
Carbon Dots ◽  
Carbon Nitride ◽  
Graphitic Carbon ◽  
Graphitic Carbon Nitride ◽  
Cross Linking ◽  
Photocatalytic Hydrogen Production

The N-modified carbon dots/graphitic carbon nitride (NCDs/g-C3N4) aerogel was successfully prepared by simple electrostatic self-assembly of NCDs and g-C3N4 nanosheets without any harmful solvents or cross-linking agents. The prepared aerogel...

Cross-linking polymerization-induced self-assembly to produce branched core cross-linked star block polymer micelles

2020 ◽  
Vol 11 (26) ◽  
pp. 4335-4343 ◽  
Author(s):  
Jongmin Park ◽  
Nam Young Ahn ◽  
Myungeun Seo
Keyword(s):  
Self Assembly ◽  
Cross Linking ◽  
Polymer Micelles ◽  
Block Polymer ◽  
Cross Linker

Copolymerizing a cross-linker in the PISA process spontaneously produces branched core cross-linked block polymer micelles.

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