A LOV-domain-mediated blue-light-activated adenylate (adenylyl) cyclase from the cyanobacterium Microcoleus chthonoplastes PCC 7420

2013 ◽  
Vol 455 (3) ◽  
pp. 359-365 ◽  
Author(s):  
Sarah Raffelberg ◽  
Linzhu Wang ◽  
Shiqiang Gao ◽  
Aba Losi ◽  
Wolfgang Gärtner ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Adenylyl Cyclase ◽  
Blue Light ◽  
Lov Domain ◽  
E Coli ◽  
Microcoleus Chthonoplastes

We describe the first adenylate cyclase with a LOV domain. The protein, which we name mPAC, originates from a cyanobacterium but is photoactivated in vertebrate cells and could be purified and functionally characterized after expression in E. coli.

scholarly journals Blue light is a universal tactic signal forEscherichia coli

2017 ◽  
Author(s):  
Tatyana Perlova ◽  
Martin Gruebele ◽  
Yann R. Chemla
Keyword(s):  
Blue Light ◽  
Light Exposure ◽  
Biological Significance ◽  
Proton Motive Force ◽  
Motive Force ◽  
Swimming Velocity ◽  
Bacterial Strains ◽  
E Coli ◽  
Before And After ◽  
Swimming Bacteria

AbstractBlue light has been shown to elicit a tumbling response inE. coli, a non-phototrophic bacterium. The exact mechanism of this phototactic response is still unknown, and its biological significance remains unclear. Here, we quantify phototaxis inE. coliby analyzing single-cell trajectories in populations of free-swimming bacteria before and after light exposure. Bacterial strains expressing only one type of chemoreceptor reveal that all fiveE. colireceptors - Aer, Tar, Tsr, Tap and Trg - are capable of mediating a response to light. In particular, light exposure elicits a running response in Tap-only strain, the opposite of the tumbling response observed for all other strains. Light therefore emerges as a universal stimulus for allE. colichemoreceptors. We also show that blue light exposure causes a reversible decrease in swimming velocity, a proxy for proton motive force. We hypothesize that rather than sensing light directly, chemoreceptors sense light-induced perturbations in proton motive force.ImportanceOur findings provide new insights on the mechanism ofE. coliphototaxis, showing that all five chemoreceptor types respond to light and that their interactions play an important role in cell behavior. Our results also open up new avenues for examining and manipulatingE. colitaxis. Since light is a universal stimulus, it may provide a way to quantify interactions between different types of receptors. Since light is easier to control spatially and temporally than chemicals, it may be used to study swimming behavior in complex environments. Since phototaxis can cause migration ofE. colibacteria in light gradients, light may be used to control bacterial density for studying density-dependent processes in bacteria.

scholarly journals Optoregulated Drug Release from an Engineered Living Hydrogel

2018 ◽  
Author(s):  
Shrikrishnan Sankaran ◽  
Judith Becker ◽  
Christoph Wittmann ◽  
Aránzazu del Campo
Keyword(s):  
Drug Release ◽  
Blue Light ◽  
Light Irradiation ◽  
E Coli ◽  
Hydrogel Matrix ◽  
Prolonged Drug Release

A living hydrogel has been developed with metabolically and optogenetically engineered E. coli encapsulated within an agarose-based hydrogel matrix to produce and release deoxyviolacein in response to blue light irradiation. Localized, tunable and prolonged drug release have been demonstrated.<br>

A blue-light-activated adenylyl cyclase mediates photoavoidance in Euglena gracilis

Nature ◽  
2002 ◽  
Vol 415 (6875) ◽  
pp. 1047-1051 ◽  
Author(s):  
Mineo Iseki ◽  
Shigeru Matsunaga ◽  
Akio Murakami ◽  
Kaoru Ohno ◽  
Kiyoshi Shiga ◽  
...  
Keyword(s):  
Adenylyl Cyclase ◽  
Blue Light ◽  
Euglena Gracilis

scholarly journals The Influence of the Degradation of Tetracycline by Free Radicals from Riboflavin-5′-Phosphate Photolysis on Microbial Viability

2019 ◽  
Vol 7 (11) ◽  
pp. 500 ◽  
Author(s):  
Shiuh-Tsuen Huang ◽  
Shwu-Yuan Lee ◽  
Song-Hua Wang ◽  
Chun-Yi Wu ◽  
Jeu-Ming P. Yuann ◽  
...  
Keyword(s):  
Blue Light ◽  
Treatment Method ◽  
Light Irradiation ◽  
Flavin Mononucleotide ◽  
Molecular Formula ◽  
Bacterial Survival ◽  
Exact Mass ◽  
E Coli ◽  
Environmentally Sound ◽  
Antibiotic Degradation

Tetracycline (TC) is a broad-spectrum antibiotic compound. Wastewater with TC may have an adverse effect on ecosystems. Riboflavin-5′-phosphate (FMN or flavin mononucleotide) is a non-toxic product of the phosphorylation of vitamin B2 and is required for the proper functioning of the humans. FMN is sensitized to ultraviolet (UV) and blue light radiation, as evidenced by the generation of reactive oxygen species (ROS). This study inspects feasible applications of blue light on FMN so as to develop a valid way of degrading TC by FMN photolysis. We used the increased rate of bacterial survival as a practical indicator of antibiotic degradation. TC in the presence of FMN solution decomposed completely after 20 W/m2 of blue light irradiation (TCF treatment), and the degradation of TC (D-TCF) occurred after the photolytic process. After TCF treatment, colony-forming units (CFUs) of Escherichia coli (E. coli) were determined for the D-TCF solution. The CFU of E. coli preservation was 93.2% of the D-TCF solution (50 μg/mL of TC in the presence of 114 μg/mL of FMN solution treated with 20 W/m2 of blue light irradiation at 25 °C for 1 h) cultivation. The mass spectrum of D-TCF showed diagnostic ion signals at m/z 431.0 and 414.0 Da. The molecular formula of D-TCF was C21H22N2O8, and the exact mass was 430.44 g/mol. TC degradation by FMN photolysis can significantly decrease the antimicrobial ability of TC. The results expressed here regarding the influence of FMN photolysis on TC degradation offer an environmentally sound wastewater treatment method.

scholarly journals Establishment of Optogenetic Modulation of cAMP for Analyzing Growth, Biofilm Formation, and Virulence Pathways of Bacteria Using a Light-Gated Cyclase

2020 ◽  
Vol 10 (16) ◽  
pp. 5535
Author(s):  
Manish Singh Kaushik ◽  
Swaroop Ranjan Pati ◽  
Shivanika Soni ◽  
Ayushi Mishra ◽  
Kumari Sushmita ◽  
...  
Keyword(s):  
Blue Light ◽  
Biofilm Formation ◽  
Research Work ◽  
Adenosine Monophosphate ◽  
Camp Signaling ◽  
Light Illumination ◽  
Dependent Manner ◽  
Regulatory Pathways ◽  
Bacterial Physiology ◽  
E Coli

In bacteria, cyclic adenosine monophosphate (cAMP) signaling plays an essential regulatory role whose modulation via optogenetic tools would provide researchers an immense opportunity to control biological processes simply by illumination. The cAMP signaling in bacteria is a complex network of regulatory pathways, which utilizes distinct proteomic resources under different nutrient environments. We established an optogenetic modulation of cAMP and studied important cellular process of growth, biofilm formation, and virulence in the model bacterium E. coli using a light-gated adenylate cyclase (LgAC) from Beggiatoa sp. Blue light-induced activation of LgAC elevated the cAMP level in a blue light-dependent manner in E. coli. Quantitative proteomics revealed a decrease in the level of certain proteins governing growth (PTS, Adk, AckA, GlnA, and EFP), biofilm formation (IhfA, flagellin, YajQ, YeaG, and HlfC), and virulence (ClpP, YebC, KatE, BtuE, and Zur) in E. coli cells expressing LgAC upon blue light illumination. This optogenetic modulation of cAMP would be useful for deciphering cAMP-associated host–pathogen signaling of bacterial systems. Proteome knowledge established by this research work would also be useful for the scientific community while adapting LgAC-based optogenetic modulation for studying other relevant cAMP-driven bacterial physiology (e.g., energy metabolism). The systematic utilization of the established method and more extensively designed experiments regarding bacterial growth, biofilm, survival, and virulence might provide a road map for the identification of new targets for developing novel antibacterial drugs.

scholarly journals Blue Light Is a Universal Signal forEscherichia coliChemoreceptors

2019 ◽  
Vol 201 (11) ◽  
Author(s):  
Tatyana Perlova ◽  
Martin Gruebele ◽  
Yann R. Chemla
Keyword(s):  
Blue Light ◽  
Light Exposure ◽  
Proton Motive Force ◽  
Motive Force ◽  
Swimming Velocity ◽  
Bacterial Strains ◽  
Content Type ◽  
E Coli ◽  
Before And After ◽  
Swimming Bacteria

ABSTRACTBlue light has been shown to elicit a tumbling response inEscherichia coli, a nonphototrophic bacterium. The exact mechanism of this phototactic response is still unknown. Here, we quantify phototaxis inE. coliby analyzing single-cell trajectories in populations of free-swimming bacteria before and after light exposure. Bacterial strains expressing only one type of chemoreceptor reveal that all fiveE. colireceptors (Aer, Tar, Tsr, Tap, and Trg) are capable of mediating responses to light. In particular, light exposure elicits a running response in the Tap-only strain, the opposite of the tumbling responses observed for all other strains. Therefore, light emerges as a universal stimulus for allE. colichemoreceptors. We also show that blue light exposure causes a reversible decrease in swimming velocity, a proxy for proton motive force. This result is consistent with a previously proposed hypothesis that, rather than sensing light directly, chemoreceptors sense light-induced perturbations in proton motive force, although other factors are also likely to contribute.IMPORTANCEOur findings provide new insights into the mechanism ofE. coliphototaxis, showing that all five chemoreceptor types respond to light and their interactions play an important role in cell behavior. Our results also open up new avenues for examining and manipulatingE. colitaxis. Since light is a universal stimulus, it may provide a way to quantify interactions among different types of receptors. Because light is easier to control spatially and temporally than chemicals, it may be used to study swimming behavior in complex environments. Since phototaxis can cause migration ofE. colibacteria in light gradients, light may be used to control bacterial density for studying density-dependent processes in bacteria.

scholarly journals Regulation of adenylate cyclase in E. coli.

1982 ◽  
Vol 1 (3) ◽  
pp. 333-337 ◽  
Author(s):  
E. Gstrein-Reider ◽  
M. Schweiger
Keyword(s):  
Adenylate Cyclase ◽  
E Coli

Engineered Arabidopsis Blue Light Receptor LOV Domain Variants with Improved Quantum Yield, Brightness, and Thermostability

2019 ◽  
Vol 67 (43) ◽  
pp. 12037-12043 ◽  
Author(s):  
Sanghwan Ko ◽  
Bora Hwang ◽  
Jung-Hyun Na ◽  
Jisun Lee ◽  
Sang Taek Jung
Keyword(s):  
Quantum Yield ◽  
Blue Light ◽  
Lov Domain ◽  
Blue Light Receptor ◽  
Light Receptor
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