Zebrafish: gaining popularity in lipid research

2010 ◽  
Vol 429 (2) ◽  
pp. 235-242 ◽  
Author(s):  
Maarit Hölttä-Vuori ◽  
Veijo T. V. Salo ◽  
Lena Nyberg ◽  
Christian Brackmann ◽  
Annika Enejder ◽  
...  
Keyword(s):  
Genetic Manipulation ◽  
Imaging Techniques ◽  
Low Cost ◽  
Rapid Development ◽  
Model Organism ◽  
Lipoprotein Metabolism ◽  
Lipid Absorption ◽  
Whole Body ◽  
Lipid Research ◽  
Biological Phenomena

Zebrafish are an increasingly popular vertebrate model organism in which to study biological phenomena. It has been widely used, especially in developmental biology and neurobiology, and many aspects of its development and physiology are similar to those of mammals. The popularity of zebrafish relies on its relatively low cost, rapid development and ease of genetic manipulation. Moreover, the optical transparency of the developing fish together with novel imaging techniques enable the direct visualization of complex phenomena at the level of the entire organism. This potential is now also being increasingly appreciated by the lipid research community. In the present review we summarize basic information on the lipid composition and distribution in zebrafish tissues, including lipoprotein metabolism, intestinal lipid absorption, the yolk lipids and their mobilization, as well as lipids in the nervous system. We also discuss studies in which zebrafish have been employed for the visualization of whole-body lipid distribution and trafficking. Finally, recent advances in using zebrafish as a model for lipid-related diseases, including atherosclerosis, obesity, diabetes and hepatic steatosis are highlighted. As the insights into zebrafish lipid metabolism increase, it is likely that zebrafish as a model organism will become an increasingly powerful tool in lipid research.

scholarly journals Mask R-CNN Based C. Elegans Detection with a DIY Microscope

Biosensors ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 257
Author(s):  
Sebastian Fudickar ◽  
Eike Jannik Nustede ◽  
Eike Dreyer ◽  
Julia Bornhorst
Keyword(s):  
Cell Biology ◽  
High Throughput Screening ◽  
Low Cost ◽  
Image Acquisition ◽  
Rapid Development ◽  
Model Organism ◽  
Large Data ◽  
Data Set ◽  
C Elegans ◽  
Do It Yourself

Caenorhabditis elegans (C. elegans) is an important model organism for studying molecular genetics, developmental biology, neuroscience, and cell biology. Advantages of the model organism include its rapid development and aging, easy cultivation, and genetic tractability. C. elegans has been proven to be a well-suited model to study toxicity with identified toxic compounds closely matching those observed in mammals. For phenotypic screening, especially the worm number and the locomotion are of central importance. Traditional methods such as human counting or analyzing high-resolution microscope images are time-consuming and rather low throughput. The article explores the feasibility of low-cost, low-resolution do-it-yourself microscopes for image acquisition and automated evaluation by deep learning methods to reduce cost and allow high-throughput screening strategies. An image acquisition system is proposed within these constraints and used to create a large data-set of whole Petri dishes containing C. elegans. By utilizing the object detection framework Mask R-CNN, the nematodes are located, classified, and their contours predicted. The system has a precision of 0.96 and a recall of 0.956, resulting in an F1-Score of 0.958. Considering only correctly located C. elegans with an [email protected] IoU, the system achieved an average precision of 0.902 and a corresponding F1 Score of 0.906.

scholarly journals Developing Technologies for Biological Experiments in Deep Space

Proceedings ◽  
2020 ◽  
Vol 60 (1) ◽  
pp. 28 ◽  
Author(s):  
Elizabeth M. Hawkins ◽  
Ada Kanapskyte ◽  
Sergio R. Santa Maria
Keyword(s):  
Low Cost ◽  
Model Organism ◽  
Low Earth Orbit ◽  
Space Environment ◽  
Model Organisms ◽  
Biological Study ◽  
The Moon ◽  
Deep Space ◽  
Biological Phenomena ◽  
Space Environments

In light of an upcoming series of missions beyond low Earth orbit (LEO) through NASA’s Artemis program and the potential establishment of bases on the Moon and Mars, the effects of the deep space environment on biology need to be examined and protective countermeasures need to be developed. Even though many biological experiments have been performed in space since the 1960s, most of them have occurred in LEO and for only short periods of time. These LEO missions have studied many biological phenomena in a variety of model organisms, as well as utilized a broad range of technologies. Given the constraints of the deep space environment, however, future deep space biological missions will be limited to microbial organisms using miniaturized technologies. Small satellites like CubeSats are capable of querying relevant space environments using novel instruments and biosensors. CubeSats also provide a low-cost alternative to more complex and larger missions, and require minimal crew support, if any. Several have been deployed in LEO, but the next iteration of biological CubeSats will go farther. BioSentinel will be the first interplanetary CubeSat and the first biological study NASA has sent beyond Earth’s magnetosphere in 50 years. BioSentinel is an autonomous free-flyer platform able to support biology and to investigate the effects of radiation on a model organism in interplanetary deep space. The BioSensor payload contained within the free-flyer is also an adaptable instrument that can perform biologically relevant measurements with different microorganisms and in multiple space environments, including the ISS, lunar gateway, and on the surface of the Moon. Nanosatellites like BioSentinel can be used to study the effects of both reduced gravity and space radiation and can house different organisms or biosensors to answer specific scientific questions. Utilizing these biosensors will allow us to better understand the effects of the space environment on biology so humanity may return safely to deep space and venture farther than ever before.

Immunszintigraphie mit 111ln-DTPA-markierten monoklonalen Antikörpern: Vergleich zwischen ECT und planarer Szintigraphie

1987 ◽  
Vol 26 (06) ◽  
pp. 258-262
Author(s):  
J. Happi ◽  
R. P. Baum ◽  
J. Frohn ◽  
B. Weimer ◽  
A. Halbsguth ◽  
...  
Keyword(s):  
Imaging Techniques ◽  
Diagnostic Procedures ◽  
Undifferentiated Carcinoma ◽  
Whole Body ◽  
Acquisition Time ◽  
Planar Imaging ◽  
Primary Tumors ◽  
Intraindividual Comparison ◽  
Single Detector ◽  
Increased Sensitivity

The present study was done in order to examine if the use of111ln-DTPA- labeled MAb fragments in place of 131l-labeled MAb fragments increases the sensitivity of tomographic immunoscintigraphy to reach the level of that of planar imaging techniques. In 11 patients with various primary tumors, local recurrences or metastases [colorectal carcinoma (n = 7), ovarian carcinoma (n = 2), papillary thyroid carcinoma (n = 1), undifferentiated carcinoma of the lung (n = 1)], immunoscintigraphy (IS) was carried out using 111ln-DTPA- labeled F(ab’)2 fragments of various MAbs (anti-CEA, OC 125, anti-hTG) and planar and tomographic imaging were compared intraindividually. By conventional diagnostic procedures, the presence of a tumor mass was confirmed (transmission computer tomography, ultrasound) or verified (131l whole-body scintigraphy, histology) in all cases. Immunoscintigraphy was positive in 9 out of 11 cases by ECT and in 10 out of 11 cases by planar imaging. When using 111 In-labeled MAb fragments, intraindividual comparison of ECT and planar imaging resulted in a similar sensitivity. The increased sensitivity of ECT using this tracer in contrast to 131l-labeled MAb fragments may be attributed to the fact that the physical properties of111 In are much more suitable for the gamma cameras most commonly used (single detector, 3/8” crystal); using 111 In-labeled MAb fragments, count rates sufficient for ECT can be obtained within a reasonable acquisition time. This allows to combine IS with the advantages of ECT regarding tumour localization and prevention of artefacts due to superposition of background.

scholarly journals Research on college gymnastics teaching model based on multimedia image and image texture feature analysis

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Ying Wu ◽  
Jikun Liu
Keyword(s):  
Human Body ◽  
Rapid Development ◽  
Texture Feature ◽  
Texture Features ◽  
Dynamics Simulation ◽  
Image Texture ◽  
Whole Body ◽  
Database Modeling ◽  
Rigid Model ◽  
The Subject

AbstractWith the rapid development of gymnastics technology, novel movements are also emerging. Due to the emergence of various complicated new movements, higher requirements are put forward for college gymnastics teaching. Therefore, it is necessary to combine the multimedia simulation technology to construct the human body rigid model and combine the image texture features to display the simulation image in texture form. In the study, GeBOD morphological database modeling was used to provide the data needed for the modeling of the whole-body human body of the joint and used for dynamics simulation. Simultaneously, in order to analyze and summarize the technical essentials of the innovative action, this experiment compared and analyzed the hem stage of the cross-headstand movement of the subject and the hem stage of the 180° movement. Research shows that the method proposed in this paper has certain practical effects.

scholarly journals A Multi-Loop Vehicle-Counting Method under Gray Mode and RGB Mode

2021 ◽  
Vol 11 (15) ◽  
pp. 6831
Author(s):  
Yue Chen ◽  
Jian Lu
Keyword(s):  
Intelligent Transportation Systems ◽  
High Efficiency ◽  
Road Traffic ◽  
Low Cost ◽  
Rapid Development ◽  
Intelligent Transportation ◽  
Transportation Systems ◽  
Counting Method ◽  
Moving Vehicle ◽  
Available Information

With the rapid development of road traffic, real-time vehicle counting is very important in the construction of intelligent transportation systems (ITSs). Compared with traditional technologies, the video-based method for vehicle counting shows great importance and huge advantages in its low cost, high efficiency, and flexibility. However, many methods find difficulty in balancing the accuracy and complexity of the algorithm. For example, compared with traditional and simple methods, deep learning methods may achieve higher precision, but they also greatly increase the complexity of the algorithm. In addition to that, most of the methods only work under one mode of color, which is a waste of available information. Considering the above, a multi-loop vehicle-counting method under gray mode and RGB mode was proposed in this paper. Under gray and RGB modes, the moving vehicle can be detected more completely; with the help of multiple loops, vehicle counting could better deal with different influencing factors, such as driving behavior, traffic environment, shooting angle, etc. The experimental results show that the proposed method is able to count vehicles with more than 98.5% accuracy while dealing with different road scenes.

Time course of insulin resistance associated with feeding dogs a high-fat diet

1997 ◽  
Vol 272 (1) ◽  
pp. E147-E154 ◽  
Author(s):  
A. P. Rocchini ◽  
P. Marker ◽  
T. Cervenka
Keyword(s):  
Insulin Resistance ◽  
Blood Pressure ◽  
Glucose Uptake ◽  
Dose Response ◽  
High Fat Diet ◽  
Time Course ◽  
Rapid Development ◽  
Diet Group ◽  
Whole Body ◽  
High Fat

The current study evaluated both the time course of insulin resistance associated with feeding dogs a high-fat diet and the relationship between the development of insulin resistance and the increase in blood pressure that also occurs. Twelve adult mongrel dogs were chronically instrumented and randomly assigned to either a control diet group (n = 4) or a high-fat diet group (n = 8). Insulin resistance was assessed by a weekly, single-dose (2 mU.kg-1.min-1) euglycemic-hyperinsulinemic clamp on all dogs. Feeding dogs a high-fat diet was associated with a 3.7 +/- 0.5 kg increase in body weight, a 20 +/- 4 mmHg increase in mean blood pressure, a reduction in insulin-mediated glucose uptake [(in mumol-kg-1.min-1) decreasing from 72 +/- 6 before to 49 +/- 7 at 1 wk, 29 +/- 3 at 3 wk, and 30 +/- 2 at 6 wk of the high-fat diet, P < 0.01]. and a reduced insulin-mediated increase in cardiac output. In eight dogs (4 high fat and 4 control), the dose-response relationship of insulin-induced glucose uptake also was studied. The whole body glucose uptake dose-response curve was shifted to the right, and the rate of maximal whole body glucose uptake was significantly decreased (P < 0.001). Finally, we observed a direct relationship between the high-fat diet-induced weekly increase in mean arterial pressure and the degree to which insulin resistance developed. In summary, the current study documents that feeding dogs a high-fat diet causes the rapid development of insulin resistance that is the result of both a reduced sensitivity and a reduced responsiveness to insulin.

scholarly journals Lactose-Inducible System for Metabolic Engineering of Clostridium ljungdahlii

2014 ◽  
Vol 80 (8) ◽  
pp. 2410-2416 ◽  
Author(s):  
Areen Banerjee ◽  
Ching Leang ◽  
Toshiyuki Ueki ◽  
Kelly P. Nevin ◽  
Derek R. Lovley
Keyword(s):  
Ethanol Production ◽  
Genetic Manipulation ◽  
Electron Flow ◽  
Model Organism ◽  
Inducible Expression ◽  
Carbon Flow ◽  
Wild Type ◽  
Content Type ◽  
Microbial Electrosynthesis ◽  
Clostridium Ljungdahlii

ABSTRACTThe development of tools for genetic manipulation ofClostridium ljungdahliihas increased its attractiveness as a chassis for autotrophic production of organic commodities and biofuels from syngas and microbial electrosynthesis and established it as a model organism for the study of the basic physiology of acetogenesis. In an attempt to expand the genetic toolbox forC. ljungdahlii, the possibility of adapting a lactose-inducible system for gene expression, previously reported forClostridium perfringens, was investigated. The plasmid pAH2, originally developed forC. perfringenswith agusAreporter gene, functioned as an effective lactose-inducible system inC. ljungdahlii. Lactose induction ofC. ljungdahliicontaining pB1, in which the gene for the aldehyde/alcohol dehydrogenase AdhE1 was downstream of the lactose-inducible promoter, increased expression ofadhE130-fold over the wild-type level, increasing ethanol production 1.5-fold, with a corresponding decrease in acetate production. Lactose-inducible expression ofadhE1in a strain in whichadhE1and theadhE1homologadhE2had been deleted from the chromosome restored ethanol production to levels comparable to those in the wild-type strain. Inducing expression ofadhE2similarly failed to restore ethanol production, suggesting thatadhE1is the homolog responsible for ethanol production. Lactose-inducible expression of the four heterologous genes necessary to convert acetyl coenzyme A (acetyl-CoA) to acetone diverted ca. 60% of carbon flow to acetone production during growth on fructose, and 25% of carbon flow went to acetone when carbon monoxide was the electron donor. These studies demonstrate that the lactose-inducible system described here will be useful for redirecting carbon and electron flow for the biosynthesis of products more valuable than acetate. Furthermore, this tool should aid in optimizing microbial electrosynthesis and for basic studies on the physiology of acetogenesis.

The Design and Implementation of ECC High-Speed Encryption Engine Based on FPGA

2012 ◽  
Vol 459 ◽  
pp. 544-548 ◽  
Author(s):  
Wei Liang ◽  
Jian Bo Xu ◽  
Wei Hong Huang ◽  
Li Peng
Keyword(s):  
Data Transmission ◽  
High Speed ◽  
Lower Cost ◽  
Low Cost ◽  
Rapid Development ◽  
Security Technology ◽  
Multiplication Algorithm ◽  
Secure Data Transmission ◽  
And Performance ◽  
Computation Speed

Network security technology ensures secure data transmission in network. Meanwhile, it brings extra overhead of security system in terms of cost and performance, which seriously affects the rapid development of existing high-speed encryption systems. The existing encryption technology cannot meet the demand of high security, low cost and high real-time. For solving above problems, an ECC encryption engine architecture based on scalable public key cipher and a high-speed configurable multiplication algorithm are designed. The algorithm was tested on FPGA platform and the experiment results show that the system has better computation speed and lower cost overhead. By comparing with other systems, our system has benefits in terms of hardware overhead and encryption time ratio

An application of high-temperature superconductors YBCO to magnetic separation

2017 ◽  
Vol 31 (25) ◽  
pp. 1745001 ◽  
Author(s):  
Qiudong Guo ◽  
Peng Zhang ◽  
Lin Bo ◽  
Guibin Zeng ◽  
Dengqian Li ◽  
...  
Keyword(s):  
Magnetic Field ◽  
High Temperature ◽  
Magnetic Separation ◽  
High Magnetic Field ◽  
Low Cost ◽  
Rapid Development ◽  
High Temperature Superconductors ◽  
New Approach ◽  
Industrial Areas ◽  
Ybco Tape

With the rapid development of manufacturing technology of high temperature superconductive YB[Formula: see text]Cu3O[Formula: see text] YBCO materials and decreasing in cost of production, YBCO is marching into industrial areas with its good performances as source of high-magnetic field and rather low cost in reaching superconductivity. Based on analysis of the performance of high temperature superconductors YBCO and development of technology in superconductive magnetic separation both home and abroad, we propose a new approach of taking YBCO tape to make a solenoid as the source of a high magnetic field of magnetic separatior of ores. The paper also looks into the future of the YBCO high temperature superconductive magnetic separation from the perspective of technology and cost, as well as its applications in other industries.

Recovery Value Metals from Mixed Spent Li-Ion Batteries

2014 ◽  
Vol 540 ◽  
pp. 267-271
Author(s):  
Xin Liu ◽  
Lin Yan Li ◽  
Fan Yun Zeng ◽  
Xue Jun Wang ◽  
Sheng Ming Xu
Keyword(s):  
Solvent Extraction ◽  
Raw Materials ◽  
Low Cost ◽  
Rapid Development ◽  
Li Ion Batteries ◽  
Active Materials ◽  
Solid Ratio ◽  
Li Ion ◽  
Leaching Condition ◽  
Addition Amount

With the rapid development and wide application of Li-ion batteries, cathode materials containing value metals Co, Ni and Mn are blended by several kind of metal oxide presently for pursuing high safe stability and low cost. The composition of spent Li-ion batteries has become complicated and optimum leaching condition varied. In this paper, leaching process for the mixture of pure LiCoO2and Li (Ni1/3Co1/3Mn1/3)O2was studied. With an increase in component of LiCoO2in mixed materials, the optimum leaching condition varied as: temperature from 60°C to 90°C, H2O2addition amount from 0.54 to 0.75ml/g and liquid-solid ratio from 10 to 20. According to this result, a real mixed spent batteries materials was recovered by being leached in 2M H2SO4at temperature of 90°C, liquid-solid ratio 20 and 0.6ml/g H2O2added. The leaching efficiencies of Co, Ni, Mn, Li were 96.88%, 93.71%, 92.12%, 99.43% respectively. Cu, Al and Fe in solution were removed by precipitation and solvent extraction. Finally, Ni, Co, Mn were extracted by D2EHPA for separating with Na+and other impurities, which is used as a raw materials for preparation of cathode active materials in batteries.

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