scholarly journals AcpA, a member of the GPR1/FUN34/YaaH membrane protein family, is essential for acetate permease activity in the hyphal fungus Aspergillus nidulans

2008 ◽  
Vol 412 (3) ◽  
pp. 485-493 ◽  
Author(s):  
Xavier Robellet ◽  
Michel Flipphi ◽  
Sylvine Pégot ◽  
Andrew P. MacCabe ◽  
Christian Vélot
Keyword(s):  
Membrane Protein ◽  
Aspergillus Nidulans ◽  
Functional Characterization ◽  
Protein Family ◽  
Genome Data ◽  
Low Concentrations ◽  
Genes Encoding ◽  
Acetate Utilization ◽  
Utilization Pathway

In a previous study, alcS, a gene of the Aspergillus nidulans alc cluster, was shown to encode a protein that belongs to the GPR1/FUN34/YaaH membrane protein family. BLAST screening of the A. nidulans genome data identified additional genes encoding hypothetical proteins that could belong to this family. In this study we report the functional characterization of one of them, AN5226. Its expression is induced by ethanol and ethyl acetate (two inducers of the alc genes) and is mediated by the specific transcriptional activator of genes of the acetate-utilization pathway FacB. Growth of a null mutant (ΔAN5226) is notably affected when acetate is used as sole carbon source at low concentration and in a high pH medium, i.e. when protonated acetate, the form that can enter the cell by passive diffusion, is present in low amounts. Consistently, expression of AN5226 is also induced by acetate, but only when the latter is present at low concentrations. 14C-labelled acetate uptake experiments using germinating conidia demonstrate an essential role for AN5226 in mediated acetate transport. To our knowledge this report is the first to provide evidence for the identification of an acetate transporter in filamentous fungi. We have designated AN5226 as acpA (for acetate permease A).

scholarly journals Structural and functional characterization of a highly secreted α- l -arabinofuranosidase (GH62) from Aspergillus nidulans grown on sugarcane bagasse

2017 ◽  
Vol 1865 (12) ◽  
pp. 1758-1769 ◽  
Author(s):  
Fabiano Jares Contesini ◽  
Marcelo Vizoná Liberato ◽  
Marcelo Ventura Rubio ◽  
Felipe Calzado ◽  
Mariane Paludetti Zubieta ◽  
...  
Keyword(s):  
Aspergillus Nidulans ◽  
Sugarcane Bagasse ◽  
Functional Characterization

FUNCTIONAL CHARACTERIZATION OF AN LCCL–LECTIN DOMAIN CONTAINING PROTEIN FAMILY IN PLASMODIUM BERGHEI

2004 ◽  
Vol 90 (5) ◽  
pp. 1062-1071 ◽  
Author(s):  
Holly E. Trueman ◽  
J. Dale Raine ◽  
Laurence Florens ◽  
Johannes T. Dessens ◽  
Jacqui Mendoza ◽  
...  
Keyword(s):  
Plasmodium Berghei ◽  
Functional Characterization ◽  
Protein Family ◽  
Lectin Domain

Cloning and functional characterization of a superfamily of microbial inwardly rectifying potassium channels

2006 ◽  
Vol 26 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Si Sun ◽  
Jo Han Gan ◽  
Jennifer J. Paynter ◽  
Stephen J. Tucker
Keyword(s):  
Functional Properties ◽  
Functional Characterization ◽  
Unicellular Eukaryote ◽  
Channel Blockers ◽  
E Coli ◽  
Inwardly Rectifying Potassium Channels ◽  
Genes Encoding ◽  
Functionally Diverse ◽  
Inwardly Rectifying

Our understanding of the mammalian inwardly rectifying family of K+ channels (Kir family) has recently been advanced by X-ray crystal structures of two homologous prokaryotic orthologs (KirBac1.1 and KirBac3.1). However, the functional properties of these KirBac channels are still poorly understood. To address this problem, we cloned and characterized genes encoding KirBac orthologs from a wide variety of different prokaryotes and a simple unicellular eukaryote. The functional properties of these KirBacs were then examined by growth complementation in a K+ uptake-deficient strain of Escherichia coli (TK2420). Whereas some KirBac genes exhibited robust growth complementation, others either did not complement or showed temperature-dependent complementation including KirBac1.1 and KirBac3.1. In some cases, KirBac expression was also toxic to the growth of E. coli. The KirBac family exhibited a range of sensitivity to the K+ channel blockers Ba2+ and Cs+ as well as differences in their ability to grow on very low-K+ media, thus demonstrating major differences in their permeation properties. These results reveal the existence of a functionally diverse superfamily of microbial KirBac genes and present an excellent resource for the structural and functional analysis of this class of K+ channels. Furthermore, the complementation assay used in this study provides a simple and robust method for the functional characterization of a range of prokaryotic K+ channels that are difficult to study by traditional methods.

scholarly journals Two Members of a Network of Putative Na+/H+ Antiporters Are Involved in Salt and pH Tolerance of the Freshwater Cyanobacterium Synechococcus elongatus

2008 ◽  
Vol 190 (19) ◽  
pp. 6318-6329 ◽  
Author(s):  
Maria Billini ◽  
Kostas Stamatakis ◽  
Vicky Sophianopoulou
Keyword(s):  
Functional Characterization ◽  
Synechococcus Elongatus ◽  
Amino Acid Sequences ◽  
High Salt ◽  
Alkaline Ph ◽  
Ph Tolerance ◽  
Low Salt ◽  
Genes Encoding ◽  
Pcc 7942

ABSTRACT Synechococcus elongatus strain PCC 7942 is an alkaliphilic cyanobacterium that tolerates a relatively high salt concentration as a freshwater microorganism. Its genome sequence revealed seven genes, nha1 to nha7 (syn_pcc79420811, syn_pcc79421264, syn_pcc7942359, syn_pcc79420546, syn_pcc79420307, syn_pcc79422394, and syn_pcc79422186), and the deduced amino acid sequences encoded by these genes are similar to those of Na+/H+ antiporters. The present work focused on molecular and functional characterization of these nha genes encoding Na+/H+ antiporters. Our results show that of the nha genes expressed in Escherichia coli, only nha3 complemented the deficient Na+/H+ antiporter activity of the Na+-sensitive TO114 recipient strain. Moreover, two of the cyanobacterial strains with separate disruptions in the nha genes (Δnha1, Δnha2, Δnha3, Δnha4, Δnha5, and Δnha7) had a phenotype different from that of the wild type. In particular, ΔnhA3 cells showed a high-salt- and alkaline-pH-sensitive phenotype, while Δnha2 cells showed low salt and alkaline pH sensitivity. Finally, the transcriptional profile of the nha1 to nha7 genes, monitored using the real-time PCR technique, revealed that the nha6 gene is upregulated and the nha1 gene is downregulated under certain environmental conditions.

Cloning and characterization of two genes encoding rainbow trout homologues of the IFITM protein family

2006 ◽  
Vol 110 (3-4) ◽  
pp. 357-362 ◽  
Author(s):  
Mark C. Johnson ◽  
Amaia Sangrador-Vegas ◽  
Terry J. Smith ◽  
Michael T. Cairns
Keyword(s):  
Rainbow Trout ◽  
Protein Family ◽  
Genes Encoding

Functional characterization of the three genes encoding 1-deoxy-D-xylulose 5-phosphate synthase in maize

2011 ◽  
Vol 62 (6) ◽  
pp. 2023-2038 ◽  
Author(s):  
E. Cordoba ◽  
H. Porta ◽  
A. Arroyo ◽  
C. San Roman ◽  
L. Medina ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Genes Encoding ◽  
Phosphate Synthase
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