scholarly journals A new class (penaeidin class 4) of antimicrobial peptides from the Atlantic white shrimp (Litopenaeus setiferus) exhibits target specificity and an independent proline-rich-domain function

2004 ◽  
Vol 381 (1) ◽  
pp. 79-86 ◽  
Author(s):  
Brandon J. CUTHBERTSON ◽  
Erika E. BÜLLESBACH ◽  
Julie FIEVET ◽  
Evelyne BACHÈRE ◽  
Paul S. GROSS
Keyword(s):  
Antimicrobial Peptide ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Target Range ◽  
Target Specificity ◽  
Litopenaeus Setiferus ◽  
New Class ◽  
Rich Domain ◽  
The Pacific ◽  
Antimicrobial Function

A highly pure, chemically defined representative of a new class of antimicrobial peptide from the Atlantic white shrimp (Litopenaeus setiferus), penaeidin class 4 [Pen4-1 (penaeidin class 4 isoform 1)], was produced synthetically. Chemical synthesis was achieved by native ligation from two separate domains yielding a bioactive peptide that reflected the characteristics of native penaeidin. Synthetic Pen4-1 proved to be an effective antimicrobial peptide, particularly against the broad-spectrum pathogen Fusarium oxysporum, exhibiting a complex effect on reproductive growth at inhibitory concentrations resulting in the suppression of spore formation. Pen4-1 exhibits unique features [not previously observed for penaeidins from the Pacific white shrimp (L. vannamei)], including target-species specificity against Gram-positive bacteria, indicating a potential partitioning of antimicrobial function among this family of peptides. The proline-rich domain of penaeidin class 4 alone was an active antimicrobial peptide, having the same target range as the full-length Pen4-1. These findings indicate that the proline-rich domain of penaeidin is sufficient to confer target specificity and that divergence in this domain between classes can result in a gain in antimicrobial function as observed for the proline-rich domain of Pen4-1.

Partial replacement of fish meal with peanut meal in practical diets for the Pacific white shrimp, Litopenaeus vannamei

2011 ◽  
Vol 43 (5) ◽  
pp. 745-755 ◽  
Author(s):  
Xiang-he Liu ◽  
Ji-dan Ye ◽  
Kun Wang ◽  
Jiang-hong Kong ◽  
Wei Yang ◽  
...  
Keyword(s):  
Litopenaeus Vannamei ◽  
Fish Meal ◽  
Peanut Meal ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Partial Replacement ◽  
The Pacific

Effects of culturing the Pacific white shrimp Penaeus vannamei in “biofloc” vs “synbiotic” systems on the growth and immune system

Aquaculture ◽  
2021 ◽  
pp. 736905
Author(s):  
Aya S. Hussain ◽  
Deyaaedin A. Mohammad ◽  
Wafaa S. Sallam ◽  
Nahla M. Shoukry ◽  
D. Allen Davis
Keyword(s):  
Immune System ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Penaeus Vannamei ◽  
The Pacific

Ultrastructure of the hepatopancreas of the Pacific white shrimp, Penaeus vannamei (Crustacea: Decapoda)

1988 ◽  
Vol 68 (2) ◽  
pp. 323-337 ◽  
Author(s):  
Thomas Caceci ◽  
Kay F. Neck ◽  
Donal D H. Lewis ◽  
Raymond F. Sis
Keyword(s):  
B Cells ◽  
Plasma Membranes ◽  
Ultrastructural Level ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Penaeus Vannamei ◽  
Intact Cells ◽  
The Pacific ◽  
Total Complement ◽  
Electron Microscopes

Fourteen specimens of the hepatopancreas of the Pacific white shrimp, Penaeus vannamei, were prepared for examination with the transmission and scanning electron microscopes and with the light microscope. The histology and ultrastructure of this organ is similar to that seen in other Decapoda. At the ultrastructural level, it was observed that B-cells rupture at approximately the level of gap junctions located on the lateral plasma membranes of the cells, and discharge the contents of their large vacuoles into the intercellular space. This efflux of enzymatic material may be the mechanism by which cells are released from the wall of the tubule at the proximal end: the rupture and collapse of a B-cell may be analagous to the removal of the keystone which supports an arch. Deprived of support, and lacking structural adaptations for cohesion (there are no desmosomes or interdigitations in the epithelium) and with the intercellular material digested, the remaining intact cells collapse into the lumen of the tubule. The lysis of individual cells of all types - R-, F-, and B-cells - may contribute to the tubules’ total complement of digestive enzymes.

scholarly journals Microbiome analysis of Pacific white shrimp gut and rearing water from Malaysia and Vietnam: implications for aquaculture research and management

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5826 ◽  
Author(s):  
Muhammad Zarul Hanifah Md Zoqratt ◽  
Wilhelm Wei Han Eng ◽  
Binh Thanh Thai ◽  
Christopher M. Austin ◽  
Han Ming Gan
Keyword(s):  
16S Rrna ◽  
Microbial Diversity ◽  
Pond Water ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Rrna Gene ◽  
Early Mortality Syndrome ◽  
The Pacific ◽  
High Sequence Conservation ◽  
Clustering Approach

Aquaculture production of the Pacific white shrimp is the largest in the world for crustacean species. Crucial to the sustainable global production of this important seafood species is a fundamental understanding of the shrimp gut microbiota and its relationship to the microbial ecology of shrimp pond. This is especially true, given the recently recognized role of beneficial microbes in promoting shrimp nutrient intake and in conferring resistance against pathogens. Unfortunately, aquaculture-related microbiome studies are scarce in Southeast Asia countries despite the severe impact of early mortality syndrome outbreaks on shrimp production in the region. In this study, we employed the 16S rRNA amplicon (V3–V4 region) sequencing and amplicon sequence variants (ASV) method to investigate the microbial diversity of shrimp guts and pond water samples collected from aquaculture farms located in Malaysia and Vietnam. Substantial differences in the pond microbiota were observed between countries with the presence and absence of several taxa extending to the family level. Microbial diversity of the shrimp gut was found to be generally lower than that of the pond environments with a few ubiquitous genera representing a majority of the shrimp gut microbial diversity such as Vibrio and Photobacterium, indicating host-specific selection of microbial species. Given the high sequence conservation of the 16S rRNA gene, we assessed its veracity at distinguishing Vibrio species based on nucleotide alignment against type strain reference sequences and demonstrated the utility of ASV approach in uncovering a wider diversity of Vibrio species compared to the conventional OTU clustering approach.

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