scholarly journals Inducible nitric oxide synthase (NOS2) expressed in septic patients is nitrated on selected tyrosine residues: implications for enzymic activity

2002 ◽  
Vol 366 (2) ◽  
pp. 399-404 ◽  
Author(s):  
Sophie LANONE ◽  
Philippe MANIVET ◽  
Jacques CALLEBERT ◽  
Jean-Marie LAUNAY ◽  
Didier PAYEN ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Protein Modification ◽  
Enzymic Activity ◽  
Tyrosine Residues ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

Tyrosine nitration is a post-translational protein modification with potentially significant biological implications. In the present study we demonstrate, for the first time, that tyrosine residues of human inducible nitric oxide synthase (NOS2) can be nitrated by peroxynitrite in vitro, leading to a decreased activity. Moreover, we show that NOS2 expressed in a skeletal muscle from septic patients is nitrated on selective tyrosine residues belonging to a canonic sequence. This phenomenon could be an endogenous mechanism of in vivo modulation of NOS2 enzymic activity.

scholarly journals Induction of inducible nitric oxide synthase (iNOS) in vitro and in vivo brain microgllia

1996 ◽  
Vol 108 (supplement) ◽  
pp. 115-120
Author(s):  
Yoshihisa KITAMURA ◽  
Hideaki TAKAHASHI ◽  
Yasuji MATSUOKA ◽  
Yasuyuki NOMURA ◽  
Takashi TANIGUCHI
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

scholarly journals virB-Mediated Survival of Brucella abortus in Mice and Macrophages Is Independent of a Functional Inducible Nitric Oxide Synthase or NADPH Oxidase in Macrophages

2002 ◽  
Vol 70 (9) ◽  
pp. 4826-4832 ◽  
Author(s):  
Yao-Hui Sun ◽  
Andreas B. den Hartigh ◽  
Renato de Lima Santos ◽  
L. Garry Adams ◽  
Renée M. Tsolis
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Nadph Oxidase ◽  
Inducible Nitric Oxide Synthase ◽  
Brucella Abortus ◽  
In Vivo Models ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

ABSTRACT The Brucella abortus virB locus is required for establishing chronic infection in the mouse. Using in vitro and in vivo models, we investigated whether virB is involved in evasion of the bactericidal activity of NADPH oxidase and the inducible nitric oxide synthase (iNOS) in macrophages. Elimination of NADPH oxidase or iNOS activity in macrophages in vitro increased recovery of wild-type B. abortus but not recovery of a virB mutant. In mice lacking either NADPH oxidase or iNOS, however, B. abortus infected and persisted to the same extent as it did in congenic C57BL/6 mice up until 60 days postinfection, suggesting that these host defense mechanisms are not critical for limiting bacterial growth in the mouse. A virB mutant did not exhibit increased survival in either of the knockout mouse strains, indicating that this locus does not contribute to evasion of nitrosative or oxidative killing mechanisms in vivo.

Regulation and expression of inducible nitric oxide synthase in vitro and in vivo

1994 ◽  
Vol 109 (1-2) ◽  
pp. 107
Author(s):  
U. Malo-Ranta ◽  
J. Luoma ◽  
M. Laukkanen ◽  
T. Nikkari ◽  
S. Yla¨-Herttuala
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

scholarly journals Tissue expression of inducible nitric oxide synthase is closely associated with resistance to Leishmania major.

1994 ◽  
Vol 180 (3) ◽  
pp. 783-793 ◽  
Author(s):  
S Stenger ◽  
H Thüring ◽  
M Röllinghoff ◽  
C Bogdan
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Leishmania Major ◽  
Tissue Expression ◽  
Ifn Gamma ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

Previous studies with inhibitors of inducible nitric oxide synthase (iNOS) suggested that high-output production of nitric oxide (NO) is an important antimicrobial effector pathway in vitro and in vivo. Here, we investigated the tissue expression of iNOS in mice after infection with Leishmania major. Immunohistochemical staining with an iNOS-specific antiserum revealed that in the cutaneous lesion and draining lymph nodes (LN) of clinically resistant mice (C57BL/6), iNOS protein is found earlier during infection and in significantly higher amounts than in the nonhealing BALB/c strain. Similar differences were seen on the mRNA level as quantitated by competitive polymerase chain reaction. Anti-CD4 treatment of BALB/c mice not only induced resistance to disease, but also restored the expression of iNOS in the tissue. In situ, few or no parasites were found in those regions of the skin lesion and the draining LN which were highly positive for iNOS. By double labeling experiments, macrophages were identified as iNOS expressing cells in vivo. In the lesions of BALB/c mice, cells staining positively for transforming growth factor beta (TGF-beta), a potent inhibitor of iNOS in vitro, were strikingly more prominent than in C57BL/6, whereas no such difference was found for interleukin 4 or interferon gamma (IFN-gamma). In vitro, production of NO was approximately threefold higher in C57BL/6 than in BALB/c macrophages after stimulation with IFN-gamma. We conclude that the pronounced expression of iNOS in resistant mice is an important mechanism for the elimination of Leishmania in vivo. The relative lack of iNOS in susceptible mice might be a consequence of macrophage deactivation by TGF-beta and reduced responsiveness to IFN-gamma.

Inhibition of inducible nitric oxide synthase in vitro and in vivo by a water-soluble extract of Wendita calysina leaves

2007 ◽  
Vol 375 (6) ◽  
pp. 349-358 ◽  
Author(s):  
Stefania Marzocco ◽  
Anna Lisa Piccinelli ◽  
Luca Rastrelli ◽  
Emanuela Mazzon ◽  
Salvatore Cuzzocrea ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Water Soluble ◽  
Soluble Extract ◽  
Water Soluble Extract ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

O34. Potent and highly selective inhibition of the inducible nitric oxide synthase in vitro and in vivo by the novel imidazopyridine BYK191023

Nitric Oxide ◽  
2006 ◽  
Vol 14 (4) ◽  
pp. 11
Author(s):  
Andreas Strub ◽  
Christian Hesslinger ◽  
Martin D. Lehner ◽  
Wolf-Rüdiger Ulrich ◽  
Manfrid Eltze ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Selective Inhibition ◽  
The Novel ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide
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