scholarly journals Identification and characterization of GSTT3, a third murine Theta class glutathione transferase

2002 ◽  
Vol 366 (1) ◽  
pp. 323-332 ◽  
Author(s):  
Marjorie COGGAN ◽  
Jack U. FLANAGAN ◽  
Michael W. PARKER ◽  
Vanicha VICHAI ◽  
William R. PEARSON ◽  
...  
Keyword(s):  
Glutathione Transferase ◽  
Expressed Sequence Tag ◽  
Molecular Model ◽  
Binding Pocket ◽  
Gene Encoding ◽  
Hydrophobic Substrate ◽  
Intron Structure ◽  
Identification And Characterization ◽  
Expressed Sequence

A novel Theta class glutathione transferase (GST) isoenzyme from mouse termed mGSTT3 has been identified by analysis of the expressed sequence tag database. The gene encoding mGSTT3 is clustered with the mGSTT1 and mGSTT2 genes on chromosome 10 and has an exon/intron structure that is similar to that of the other Theta class genes. mGSTT3 is expressed strongly in the liver and to a decreasing extent in the kidney and testis. Recombinant mGSTT3-3 expressed in Escherichia coli had a substrate-specificity profile that differed significantly from that of GSTT1-1 and GSTT2-2 isoenzymes. A molecular model of mGSTT3 suggested that, in comparison with GSTT2, a decrease in volume of the hydrophobic substrate-binding site and the loss of the sulphate-binding pocket prevents its use of the GSTT2 substrate 1-menaphthyl sulphate.

scholarly journals Expressed Sequence Tag Analysis for Identification and Characterization of Sex-Related Genes in the Giant Tiger Shrimp Penaeus monodon

BMB Reports ◽  
2007 ◽  
Vol 40 (4) ◽  
pp. 501-510 ◽  
Author(s):  
Rachanimuk Preechaphol ◽  
Rungnapa Leelatanawit ◽  
Kanchana Sittikankeaw ◽  
Sirawut Klinbunga ◽  
Bavornlak Khamnamtong ◽  
...  
Keyword(s):  
Expressed Sequence Tag ◽  
Penaeus Monodon ◽  
Tiger Shrimp ◽  
Expressed Sequence Tag Analysis ◽  
Identification And Characterization ◽  
Expressed Sequence

scholarly journals Identification and Characterization of the Most Abundant Cellulases in Stylet Secretions from Globodera rostochiensis

2009 ◽  
Vol 99 (2) ◽  
pp. 194-202 ◽  
Author(s):  
Sajid Rehman ◽  
Patrick Butterbach ◽  
Herman Popeijus ◽  
Hein Overmars ◽  
Eric L. Davis ◽  
...  
Keyword(s):  
Expressed Sequence Tag ◽  
Specific Activity ◽  
Glycosyl Hydrolase ◽  
Globodera Rostochiensis ◽  
Cyst Nematodes ◽  
C Terminus ◽  
Different Types ◽  
Identification And Characterization ◽  
Expressed Sequence

Plant-parasitic cyst nematodes secrete cell wall modifying proteins during their invasion of host plants. In this study, we used a monoclonal antibody to immunopurify and to sequence the N terminus of the most abundant cellulases in stylet secretions of preparasitic juveniles of Globodera rostochiensis. The N-terminal amino acid sequence perfectly matched the sequence of an expressed sequence tag of two nearly identical genes, named Gr-eng3 and Gr-eng4, which show relatively low similarity with the previously identified Gr-eng1 and Gr-eng2 (i.e., 62% similarity and 42% identity). The recombinantly produced proteins from Gr-eng3 and Gr-eng4 demonstrated specific activity on carboxymethylcellulose, indicating that these genes encode active cellulases. To date, the cellulases in cyst nematodes are comprised of three possible domain structure variants with different types of ancillary domains at the C terminus of the glycosyl hydrolase family 5 (GHF5) domain. We used Bayesian inference to show that the phylogeny of the GHF5 domain based on currently available data suggest that the extant nematode cellulases arose through reshuffling of the GHF5 domain with different types of ancillary domains as relatively independent units. Knocking-down Gr-eng3 and Gr-eng4 using RNA interference resulted in a reduction of nematode infectivity by 57%. Our observations show that the reduced infectivity of the nematodes can be attributed to poor penetration of the host's root system at the onset of parasitism.

Characterization of expressed sequence tag-derived simple sequence repeat markers for 17 Linum species

Botany ◽  
2010 ◽  
Vol 88 (5) ◽  
pp. 537-543 ◽  
Author(s):  
Yong-Bi Fu ◽  
Gregory W. Peterson
Keyword(s):  
Simple Sequence Repeat ◽  
Expressed Sequence Tag ◽  
Sequence Repeat ◽  
Simple Sequence Repeat Markers ◽  
Linum Usitatissimum L ◽  
Evolutionary Studies ◽  
Expressed Sequence ◽  
Simple Sequence ◽  
Cultivated Flax

One major challenge in genetic and evolutionary studies of wild flax species is the lack of informative molecular markers. A set of 100 informative expressed sequence tag-derived simple sequence repeat (EST-SSR) primer pairs developed in cultivated flax ( Linum usitatissimum L.) were characterized on 35 Linum accessions representing 17 Linum species for their transferability to other Linum species. Ninety-nine primer pairs displayed scorable polymorphisms across 35 Linum samples and generated 627 bands likely from 121 loci. About 50% of the detected bands occurred only in three or fewer samples. A total of 393 bands, likely from 116 loci, were detected by 97 primer pairs in Linum bienne Mill. samples, but only up to 60 bands, likely from up to 39 loci, were revealed by 6 to 37 primer pairs in the samples of the other 15 Linum species. The L. bienne samples displayed 23.7% more EST-SSR variation than the L. usitatissimum samples. These characterized EST-SSR markers should be useful for future genetic diversity and evolutionary studies of Linum species, particularly for the progenitor of cultivated flax.

scholarly journals Identification and characterization of YLR328W, theSaccharomyces cerevisiaestructural gene encoding NMN adenylyltransferase. Expression and characterization of the recombinant enzyme

FEBS Letters ◽  
1999 ◽  
Vol 455 (1-2) ◽  
pp. 13-17 ◽  
Author(s):  
Monica Emanuelli ◽  
Francesco Carnevali ◽  
Maria Lorenzi ◽  
Nadia Raffaelli ◽  
Adolfo Amici ◽  
...  
Keyword(s):  
Recombinant Enzyme ◽  
Gene Encoding ◽  
Identification And Characterization

scholarly journals Dissecting the sugarcane expressed sequence tag (SUCEST) database: unraveling flower-specific genes

2001 ◽  
Vol 24 (1-4) ◽  
pp. 77-84 ◽  
Author(s):  
R.C. Figueiredo ◽  
M.S. Brito ◽  
L.H.M. Figueiredo ◽  
A.C. Quiapin ◽  
P.M. Vitorelli ◽  
...  
Keyword(s):  
Expressed Sequence Tag ◽  
Tissue Expression ◽  
Reproductive Organs ◽  
Cdna Libraries ◽  
Computer Assisted ◽  
Transcript Accumulation ◽  
Gene Encoding ◽  
Assisted Analysis ◽  
Expressed Sequence ◽  
Different Tissues

There are almost 260,000 independent clones sequenced from the 5’ end in the Sugarcane Expressed Sequence Tag (SUCEST) database, which have been obtained from 37 cDNA libraries prepared from different tissues. This large number of expressed sequence tags (ESTs) provides an opportunity, unprecedented in plants, to perform ‘digital differential screening’ on selected cDNA libraries. In general, the frequency of a particular EST correlates with transcript accumulation in the tissues from which the cDNA libraries were constructed, so it is possible to compare the whole transcriptome from different tissues using computer-assisted analysis of an EST database. In our research we analyzed sugarcane ESTs according to tissue expression and identified more than 1,000 putative flower-specific genes. The fact that using this technique we were able to identify sugarcane homologues of several genes previously described as pollen-specific justifies this method of assessing tissue specificity. In addition, ESTs similar to genes specific to reproductive organs were detected e.g. a sugarcane gene encoding a meiotic protein essential for assembly of the synaptonemal complex and normal synapsis. This approach also allowed the identification of many flower-specific anonymous sequences that are good candidates for being novel genes involved in plant reproduction. This paper describes the analysis of the gene expression levels of 24 EST clusters during flower development using a ‘digital northern blot’ constructed from direct EST counts made on the non-normalized sugarcane cDNA libraries.

scholarly journals Identification and Characterization of MicroRNAs inMacaca fascicularisby EST Analysis

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Hao Yang ◽  
Haiyang Zhang ◽  
Lin Zhu ◽  
Chenyu Zhang ◽  
Donghai Li
Keyword(s):  
Macaca Fascicularis ◽  
Expressed Sequence Tag ◽  
Est Analysis ◽  
Small Noncoding Rnas ◽  
Novel Mirna ◽  
Medical Experiments ◽  
Alternative Approach ◽  
Model Animal ◽  
Identification And Characterization

MicroRNAs (miRNAs) are small noncoding RNAs which repress gene expression at the posttranscriptional level. In this study, an expressed sequence tag (EST)-based combined method was applied for the detection of miRNAs inMacaca fasciculariswhich is used as a model animal extensively in medical experiments, particularly those involved with neuroscience and disease. Initially, previously known miRNA sequences from metazoans were used to blast with the EST databases ofMacaca fascicularis, and then a range of filtering criteria was conducted to remove some pseudo ones. At last a total of 8 novel conserved miRNAs were identified; their functions were further predicted and analyzed. Together, our study provides insight into miRNAs and their functions inMacaca fascicularis, indicating that the EST analysis is an efficient and affordable alternative approach for identifying novel miRNA candidates.

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