scholarly journals Hydrolysable ATP is a requirement for the correct interaction of molecular chaperonins cpn60 and cpn10

2002 ◽  
Vol 364 (3) ◽  
pp. 849-855 ◽  
Author(s):  
Chris WALTERS ◽  
Neil ERRINGTON ◽  
Arther J. ROWE ◽  
Stephen E. HARDING
Keyword(s):  
Light Scattering ◽  
Molecular Chaperone ◽  
Analytical Ultracentrifugation ◽  
Sedimentation Velocity ◽  
Dilute Solution ◽  
Binding Ability ◽  
Absorption Spectrophotometry ◽  
Molecular Hydrodynamic ◽  
Atp Analogues ◽  
Bead Modelling

Over recent years the binding ability of the molecular chaperone cpn60 (GroEL14) and its co-chaperone cpn10 (GroES7) has been reported to occur under an assortment of specific conditions from the use of non-hydrolysable ATP analogues (namely adenosine 5′-[γ-thio]triphosphate) to requiring hydrolysable ATP for any interaction to occur. We have investigated this further using the molecular hydrodynamic methods (hydrodynamic bead modelling, sedimentation-velocity analytical ultracentrifugation and dynamic light-scattering), allowing the process to be followed under physiologically relevant dilute solution conditions, combined with absorption spectrophotometry to determine GroES7—GroEL14 interaction through the rate inhibition of the cpn60's ATPase activity by GroES7. The results found here indicate that the presence of hydrolysable ATP is required to facilitate correct GroES7 interaction with GroEL14 in solution.

scholarly journals Self-association of proteoglycan subunits from pig laryngeal cartilage

1978 ◽  
Vol 171 (1) ◽  
pp. 109-114 ◽  
Author(s):  
J K Sheehan ◽  
I A Nieduszynski ◽  
C F Phelps
Keyword(s):  
Light Scattering ◽  
Ionic Strength ◽  
Guanidine Hydrochloride ◽  
Sedimentation Velocity ◽  
Dilute Solution ◽  
Thiol Groups ◽  
Dimer Model ◽  
Laryngeal Cartilage ◽  
Self Association ◽  
Corroborative Evidence

Proteoglycans from pig laryngeal cartilage prepared by dissociative extraction in guanidine hydrochloride were studied in dilute solution by light-scattering and ultracentrifugation. In buffered 150mM-NaCl, pH7.4, the proteoglycan particle weights were about 5×10(6) daltons, but at 100mM-, 200mM- and 300mM-NaCl particle weights of 2.5×10(6)–3.0×10(6) daltons were observed. These results, together with corroborative evidence from sedimentation-velocity experiments, were interpreted in terms of proteoglycans self-associating at physiological ionic strength. The data were examined by using a proteoglycan monomer-dimer model. Proteoglycan preparations that had thiol groups partially carboxymethylated gave particle weights of 3.2×10(6)–3.5×10(6) daltons in 150mM-NaCl, which suggested that carboxymethylation inhibited multimerization and hence that the protein core is implicated in the binding site. Further studies showed that the multimers were stable to 60 degrees C, unlike the hyaluronate-proteoglycan complex.

scholarly journals Terpene polyacrylate TPA5 shows favorable molecular hydrodynamic properties as a potential bioinspired archaeological wood consolidant

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Michelle Cutajar ◽  
Fabrizio Andriulo ◽  
Megan R. Thomsett ◽  
Jonathan C. Moore ◽  
Benoit Couturaud ◽  
...  
Keyword(s):  
Molar Mass ◽  
Analytical Ultracentrifugation ◽  
Gel Filtration ◽  
Axial Ratio ◽  
Sedimentation Equilibrium ◽  
Archaeological Wood ◽  
Molecular Hydrodynamic ◽  
Pine Trees ◽  
Minimal Concentration ◽  
Hydrogen Bonding Potential

AbstractThere is currently a pressing need for the development of novel bioinspired consolidants for waterlogged, archaeological wood. Bioinspired materials possess many advantages, such as biocompatibility and sustainability, which makes them ideal to use in this capacity. Based on this, a polyhydroxylated monomer was synthesised from α-pinene, a sustainable terpene feedstock derived from pine trees, and used to prepare a low molar mass polymer TPA5 through free radical polymerisation. This polymer was extensively characterised by NMR spectroscopy (chemical composition) and molecular hydrodynamics, primarily using analytical ultracentrifugation reinforced by gel filtration chromatography and viscometry, in order to investigate whether it would be suitable for wood consolidation purposes. Sedimentation equilibrium indicated a weight average molar mass Mw of (4.3 ± 0.2) kDa, with minimal concentration dependence. Further analysis with MULTISIG revealed a broad distribution of molar masses and this heterogeneity was further confirmed by sedimentation velocity. Conformation analyses with the Perrin P and viscosity increment ν universal hydrodynamic parameters indicated that the polymer had an elongated shape, with both factors giving consistent results and a consensus axial ratio of ~ 4.5. These collective properties—hydrogen bonding potential enhanced by an elongated shape, together with a small injectable molar mass—suggest this polymer is worthy of further consideration as a potential consolidant.

Sedimentation velocity analytical ultracentrifugation and SEDFIT/c(s): Limits of quantitation for a monoclonal antibody system

2007 ◽  
Vol 361 (1) ◽  
pp. 24-30 ◽  
Author(s):  
John P. Gabrielson ◽  
Theodore W. Randolph ◽  
Brent S. Kendrick ◽  
Michael R. Stoner
Keyword(s):  
Monoclonal Antibody ◽  
Analytical Ultracentrifugation ◽  
Sedimentation Velocity

Aggregation behaviour of stereoregular poly(methyl methacrylates) in dilute solution: light scattering and proton n.m.r. study

Polymer ◽  
1983 ◽  
Vol 24 (6) ◽  
pp. 700-706 ◽  
Author(s):  
L. Mrkvičková ◽  
J. Stêjskal ◽  
J. Spěváček ◽  
J. Horská ◽  
O. Quadrat
Keyword(s):  
Light Scattering ◽  
Dilute Solution ◽  
Aggregation Behaviour ◽  
Methyl Methacrylates
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