scholarly journals Effect of the α-agonist noradrenaline on total and 45Ca2+ movements in mitochondria of rat liver cells

1981 ◽  
Vol 200 (1) ◽  
pp. 177-180 ◽  
Author(s):  
Brigitte Berthon ◽  
Josiane Poggioli ◽  
Thierry Capiod ◽  
Michel Claret
Keyword(s):  
Rat Liver ◽  
Liver Cells ◽  
Isolated Cells ◽  
Experimental Conditions ◽  
Rat Liver Cells ◽  
Rat Livers ◽  
In Cells

Ca2+ movements triggered by noradrenaline were determined in isolated cells and mitochondria from rat livers. It has been shown that these depend on experimental conditions. In cells incubated in 1.8mm-Ca2+, results suggest that noradrenaline mobilizes Ca2+ from reticulum before releasing Ca2+ from mitochondria.

scholarly journals Actin and Vimentin proteins with N-terminal deletion detected in tumor-bearing rat livers induced by intraportal-vein injection of Ha-ras-transfected rat liver cells

2008 ◽  
Vol 124 (11) ◽  
pp. 2512-2519 ◽  
Author(s):  
Yasushi Nakamura ◽  
Akari Kominami ◽  
Yoshiyuki Tsujimoto ◽  
Yuko Nakayama ◽  
Tsukasa Kitahashi ◽  
...  
Keyword(s):  
Rat Liver ◽  
Liver Cells ◽  
Terminal Deletion ◽  
Tumor Bearing ◽  
Rat Liver Cells ◽  
Rat Livers

scholarly journals Polyribosomes in isolated liver cells. Preparative procedures, effects of incubation and correlation with protein synthesis

1980 ◽  
Vol 186 (1) ◽  
pp. 35-45 ◽  
Author(s):  
A J Dickson ◽  
C I Pogson
Keyword(s):  
Protein Synthesis ◽  
Rat Liver ◽  
Isolated Hepatocytes ◽  
Liver Cells ◽  
Liver Protein ◽  
Isolated Cells ◽  
Isolated Liver Cells ◽  
Rat Liver Cells ◽  
Isolated Liver

Methods have been derived which permit the isolation of undergraded polyribosomes from isolated rat liver cells. Under the conditions used the polyribosome profile of hepatocytes immediately after isolation was essentially identical with that from intact liver. However, during incubation of cells in complex physiological media there was a progressive dissociation of polyribosomes. The addition of a variety of factors that produce reaggregation of polyribosomes in rat liver in vivo did not prevent dissociation during cell incubations. Although large polyribosomes were lost most rapidly, the albumin-synthesizing capacity of isolated cells was not selectively lost when compared with total protein synthesis. The significance of these results for the use of isolated hepatocytes in the study of liver protein synthesis is discussed.

scholarly journals The effects L-leucine on the synthesis of urea, glutamate and glutamine by isolated rat liver cells

1975 ◽  
Vol 146 (2) ◽  
pp. 457-464 ◽  
Author(s):  
J M Mourão ◽  
J D McGivan ◽  
J B Chappell
Keyword(s):  
Amino Acids ◽  
Nitrogen Source ◽  
Glutamate Dehydrogenase ◽  
Rat Liver ◽  
Liver Cells ◽  
Carbamoyl Phosphate ◽  
Isolated Cells ◽  
Isolated Rat Liver ◽  
Rat Liver Cells ◽  
Isolated Rat

With either alanine or a mixture of 15 different amino acids as nitrogen source, the addition of L-leucine inhibited the synthesis of urea by isolated rat liver cells. With alanine present leucine promoted the production of glutamate and glutamine. Comparison of effects of leucine on soluble glutamate dehydrogenase, mitochondria and isolated cells supports the postulate that leucine exerts its effect through activation of glutamate dehydrogenase. It is suggested that this latter enzyme may not be as important for the production of NH3 for carbamoyl phosphate synthesis as has been considered hitherto.

Activation of Na+- and Ca2+-dependent Mg2+ extrusion by α1- and β-adrenergic agonists in rat liver cells

2000 ◽  
Vol 279 (5) ◽  
pp. G943-G950 ◽  
Author(s):  
Theresa E. Fagan ◽  
Andrea Romani
Keyword(s):  
Adrenergic Receptor ◽  
Liver Cells ◽  
Adrenergic Agonists ◽  
Experimental Conditions ◽  
Rat Liver Cells ◽  
Rat Livers ◽  
Cellular Ca ◽  
Adrenergic Receptor Agonist ◽  
Dependent Mechanism ◽  
Stimulation Of

The administration of selective α1 (phenylephrine)-, β (isoproterenol)-, or mixed (epinephrine) adrenergic agonists induces a marked Mg2+extrusion from perfused rat livers. In the absence of extracellular Ca2+, phenylephrine does not induce a detectable Mg2+ extrusion, isoproterenol-induced Mg2+mobilization is unaffected, and epinephrine induces a net Mg2+ extrusion that is lower than in the presence of extracellular Ca2+ and quantitatively similar to that elicited by isoproterenol. In the absence of extracellular Na+, no Mg2+ is extruded from the liver irrespective of the agonist used. Similar results are observed in perfused livers stimulated by glucagon or 8-chloroadenosine 3′,5′-cyclic monophosphate. In the absence of extracellular Na+ or Ca2+, adrenergic-induced glucose extrusion from the liver is also markedly decreased. Together, these results indicate that liver cells extrude Mg2+ primarily via a Na+-dependent mechanism. This extrusion pathway can be activated by the increase in cellular cAMP that follows the stimulation by glucagon or a specific β-adrenergic receptor agonist or, alternatively, by the changes in cellular Ca2+ induced by the stimulation of the α1-adrenoceptor. In addition, the stimulation of the α1-adrenoceptor appears to activate an auxiliary Ca2+-dependent Mg2+extrusion pathway. Finally, our data suggest that experimental conditions that affect Mg2+ mobilization also interfere with glucose extrusion from liver cells.

Effects of Hypophysectomy on the Fine Structure of Rat Liver Cells

1967 ◽  
Vol 25 ◽  
pp. 156-157
Author(s):  
Robert R. Cardell
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Rat Liver ◽  
Liver Cell ◽  
Anterior Pituitary ◽  
Liver Cells ◽  
Biochemical Studies ◽  
Liver Functions ◽  
Rat Liver Cells ◽  
And Control

Hypophysectomy of the rat renders this animal deficient in the hormones of the anterior pituitary gland, thus causing many primary and secondary hormonal effects on basic liver functions. Biochemical studies of these alterations in the rat liver cell are quite extensive; however, relatively few morphological observations on such cells have been recorded. Because the available biochemical information was derived mostly from disrupted and fractionated liver cells, it seemed desirable to examine the problem with the techniques of electron microscopy in order to see what changes are apparent in the intact liver cell after hypophysectomy. Accordingly, liver cells from rats which had been hypophysectomized 5-120 days before sacrifice were studied. Sham-operated rats served as controls and both hypophysectomized and control rats were fasted 15 hours before sacrifice.

Heterogeneity of carboxylesterases in rat liver cells

1992 ◽  
Vol 44 (4) ◽  
pp. 827-829 ◽  
Author(s):  
Rolf Gaustad ◽  
Trond Berg ◽  
Frode Fonnum
Keyword(s):  
Rat Liver ◽  
Liver Cells ◽  
Rat Liver Cells
Sign in / Sign up

Export Citation Format

Share Document